Proteintech

HSP90AB1 Polyclonal Antibody

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HSP90AB1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using HSP90AB1 antibody 11405-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

HSP90AB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 11405-1-AP (HSP90AB1 antibody) at dilution of 1:500 (under 40x lens) heat mediated antigen retrieved with Sodium Citrate buffer (pH 6).

HSP90AB1 Antibody in Western Blot (WB) — Rat heart tissue were subjected to SDS PAGE followed by western blot with 11405-1-AP (HSP90AB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

HSP90AB1 Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 0.2ug HSP90AB1 antibody (11405-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

HSP90AB1 Antibody in Immunoprecipitation (IP) — IP result of anti-HSP90AB1 (IP:11405-1-AP, 4ug; Detection:11405-1-AP 1:700) with NIH/3T3 cells lysate 1200ug.

Product Details

11405-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000-1:10,000	View 4 publications 4 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:250-1:1,000	-
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Deer, Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HSP90AB1 Fusion Protein Ag1947 (330-710 aa encoded by BC014485) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.13 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: RALLFIPRR APFDLFENKK KKNNIKLYVR RVFIMDSCDE LIPEYLNFIR GVVDSEDLPL NISREMLQQS KILKVIRKNI VKKCLELFSE LAEDKENYKK FYEAFSKNLK LGIHEDSTNR RRLSELLRYH TSQSGDEMTS LSEYVSRMKE TQKSIYYITG ESKEQVANSA FVERVRKRGF EVVYMTEPID EYCVQQLKEF DGKSLVSVTK EGLELPEDEE EKKKMEESKA KFENLCKLMK EILDKKVEKV TISNRLVSSP CCIVTSTYGW TANMERIMKA QALRDNSTMG YMMAKKHLEI NPDHPIVETL RQKAEADKND KAVKDLVVLL FETALLSSGF SLEDPQTHSN RIYRMIKLGL GIDEDEVAAE EPNAAVPDEI PP (330-710 aa encoded by BC014485)

Target Information

Heat shock proteins (HSP) are expressed in response to various biological stresses, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, aryl hydrocarbon (Ah) receptor, and some kinases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Quantitative proteomic analysis of deer antler stem cells as a model of mammalian organ regeneration.

Journal of proteomics

Dong Z,Coates D,Liu Q,Sun H,Li C

Wed Mar 20 00:00:00 EDT 2019

Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer.

Oncotarget

Zhang L,Yu Z,Wang Y,Wang X,Zhang L,Wang C,Yue Q,Wang X,Deng S,Huo X,Tian X,Huang S,Zhang B,Ma X

Tue Nov 22 00:00:00 EST 2016

Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer.

Oncotarget

Zhang L,Yu Z,Wang Y,Wang X,Zhang L,Wang C,Yue Q,Wang X,Deng S,Huo X,Tian X,Huang S,Zhang B,Ma X

Tue Nov 22 00:00:00 EST 2016

Phosphoproteome Characterization of Human Colorectal Cancer SW620 Cell-Derived Exosomes and New Phosphosite Discovery for C-HPP.

Journal of proteome research

Guo J,Cui Y,Yan Z,Luo Y,Zhang W,Deng S,Tang S,Zhang G,He QY,Wang T

Fri Nov 04 00:00:00 EDT 2016

Celastrol, an inhibitor of heat shock protein 90β potently suppresses the expression of matrix metalloproteinases, inducible nitric oxide synthase and cyclooxygenase-2 in primary human osteoarthritic chondrocytes.

European journal of pharmacology

Ding QH,Cheng Y,Chen WP,Zhong HM,Wang XH

Wed May 15 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: Heat shock 84 kDa; heat shock 90kD protein 1, beta; heat shock 90kDa protein 1, beta; Heat shock protein; heat shock protein 1, beta; heat shock protein 90 kDa; heat shock protein 90kDa alpha (cytosolic), class B member 1; heat shock protein 90kDa alpha family class B member 1; Heat shock protein HSP 90-beta; heat shock protein, 84 kDa 1; HSP; HSP 84; HSP 90; HSP84; HSP90-beta; retinal degeneration slow protein; RP1-302G2.1; TSTA; Tumor-specific transplantation 84 kDa antigen

Gene Aliases: 90kDa; AL022974; C81438; D6S182; HSP84; Hsp84-1; Hsp90; HSP90-BETA; HSP90AB1; HSP90B; HSPC2; HSPCB

UniProt ID: (Human) P08238, (Mouse) P11499, (Rat) P34058

Entrez Gene ID: (Human) 3326, (Mouse) 15516, (Rat) 301252

Molecular Function: Hsp90 family chaperone

Function(s)

placenta development protein folding response to unfolded protein response to salt stress virion attachment to host cell regulation of protein ubiquitination positive regulation of protein binding negative regulation of proteasomal ubiquitin-dependent protein catabolic process positive regulation of phosphoprotein phosphatase activity positive regulation of protein import into nucleus, translocation Fc-gamma receptor signaling pathway involved in phagocytosis negative regulation of neuron apoptotic process positive regulation of nitric oxide biosynthetic process positive regulation of cell size protein stabilization regulation of interferon-gamma-mediated signaling pathway regulation of type I interferon-mediated signaling pathway cellular response to interleukin-4 cellular response to organic cyclic compound positive regulation of protein serine/threonine kinase activity fibril organization cell-cell adhesion regulation of cellular response to heat response to stress

Process(es)

UTP binding CTP binding double-stranded RNA binding protein binding ATP binding GTP binding sulfonylurea receptor binding protein kinase regulator activity kinase binding protein kinase binding MHC class II protein complex binding nitric-oxide synthase regulator activity TPR domain binding dATP binding histone deacetylase binding ion channel binding poly(A) RNA binding unfolded protein binding cadherin binding involved in cell-cell adhesion nucleotide binding

HSP90AB1 Polyclonal Antibody

Product Details

11405-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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