Invitrogen

HSPA9 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (32) HSPA9 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

HSPA9 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of HSPA9 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with HSPA9 Polyclonal Antibody (Product # PA5-78009) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing staining in cytoplasm and nucleus. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

HSPA9 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — HSPA9 Polyclonal Antibody detects Grp75 protein at mitochondria in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. HSPA9 Polyclonal Antibody (Product # PA5-78009) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

HSPA9 Antibody in Western Blot (WB) — Western blot was performed using Anti-HSPA9 Polyclonal Antibody, (Product # PA5-78009) and a 75 kDa band corresponding to HSPA9 was observed in the cell lines and tissues tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), A-431 (Lane 2), K-562 (Lane 3), COS-7 (Lane 4), Hep G2 (Lane 5), MCF7 (Lane 6), Mouse brain (Lane 7), Mouse liver (Lane 8) and Rat liver (Lane 9) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:3000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

HSPA9 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of GRP75 was performed in HepG2 whole cell extracts using 5 µg of HSPA9 Polyclonal Antibody (Product # PA5-78009). Samples were transferred to a membrane and probed with HSPA9 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.

HSPA9 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with HSPA9 siRNA and decrease in signal intensity was observed in western blot application using HSPA9 Polyclonal Antibody (Product # PA5-78009).

Product Details

PA5-78009

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Immunoprecipitation (IP)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Grp75. The exact sequence is proprietary.

Conjugate

Unconjugated

Form

Liquid

Concentration

0.42 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2735928

Product Specific Information

Positive Control: 293T, A431, HeLa, HepG2, SCC-15, IPEC-J2

Predicted Reactivity: Zebrafish (87%), Xenopus laevis (94%), Chicken (95%), Bovine (99%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The HSP70 family is composed of four highly conserved proteins: HSP70, HSC70, GRP75 and GRP78. These proteins serve a variety of roles. GRP75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP75 and GRP78 are unresponsive to heat stress and are induced by glucose deprivation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: 75 kDa glucose-regulated protein; C3H-specific antigen; catecholamine-regulated protein 40; epididymis secretory sperm binding protein Li 124m; GRP 75; GRP-75; Heat shock 70 kDa protein 9; heat shock 70kD protein 9B; heat shock 70kDa protein 9 (mortalin); heat shock 70kDa protein 9A; Heat shock protein; heat shock protein 9A; heat shock protein cognate 74; heat shock protein, 74 kDa, A; heat shock protein, A; HSP; MGC4500; Mortalin; mortalin, perinuclear; mortalin-2; mortalin2; MOT; mtHSP70; p66 MOT; p66-mortalin; PBP74; Peptide-binding protein 74; Stress-70 protein, mitochondrial

Gene Aliases: 74kDa; CRP40; CSA; EVPLS; GRP-75; GRP75; HEL-S-124m; Hsc74; Hsp74; Hsp74a; HSPA9; Hspa9a; HSPA9B; Mortalin; MOT; Mot-2; MOT2; mt-HSP70; Mthsp70; MTHSP75; PBP74; SAAN; SIDBA4

UniProt ID: (Human) P38646, (Mouse) P38647

Entrez Gene ID: (Human) 3313, (Pig) 100521183, (Rat) 291671, (Mouse) 15526

Function(s)

protein folding protein export from nucleus response to toxic substance cellular response to interleukin-1 erythrocyte differentiation negative regulation of apoptotic process negative regulation of erythrocyte differentiation

Process(es)

protein binding ATP binding fibroblast growth factor binding heat shock protein binding ubiquitin protein ligase binding poly(A) RNA binding unfolded protein binding enzyme binding nucleotide binding