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Invitrogen
HSPB8 Polyclonal Antibody
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FIGURE: 1 / 8
HSPB8 Antibody (PA5-79413) in ICC/IF
Immunocytochemistry analysis of HSPB8/Hsp22 using anti-HSPB8/Hsp22 antibody (Product # PA5-79413). HSPB8/Hsp22 was detected in a section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-HSPB8/Hsp22 antibody (Product # PA5-79413) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was coun... View More
Product Details
PA5-79413
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human HSPB8/Hsp22 recombinant protein (Position: M1-T196).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human MCF-7 whole cell, human U87 whole cell, rat H9C2 whole cell, rat heart tissue, mouse C2C12 whole cell, mouse skeletal muscle tissue, mouse heart tissue. IHC: mouse pancreas tissue, rat pancreas tissue, human lung cancer tissue, human mammary cancer tissue. ICC/IF: MCF7 cell. Flow: U2OS cell IP: MCF-7 whole cell.
Target Information
The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alpha-crystallin C chain; Alternatively spliced isoform of human HSPB8 protein; crystallin, alpha C; DHMN 2; E2-induced gene 1 protein; heat shock 22kDa protein 8; heat shock 27kDa protein 8; Heat shock protein; heat shock protein 8; Heat shock protein beta-8; Heat shock protein family B member 8; HMN 2; HSB8; HSP; HSPB 8; HSPB8; OTTHUMP00000239768; Protein kinase H11; Small stress protein-like protein HSP22; unnamed protein product
Gene Aliases: AU018630; AW413033; CMT2L; CRYAC; D5Ucla4; DHMN2; E2IG1; H11; H11K; HMN2; HMN2A; HMND2; HSP20-like; HSP22; HSPB8; HSPB8-N1; HSPB8-N2; MFM13; PP1629
UniProt ID: (Human) Q9UJY1, (Rat) Q9EPX0, (Mouse) Q9JK92
Entrez Gene ID: (Human) 26353, (Rat) 113906, (Mouse) 80888
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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