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Invitrogen
HSPE1 Polyclonal Antibody
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FIGURE: 1 / 6
HSPE1 Antibody (PA5-30428) in ICC/IF
HSPE1 Polyclonal Antibody detects Cpn10 protein at mitochondria by immunofluorescent analysis. Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 15 min. Green: Cpn10 protein stained by HSPE1 Polyclonal Antibody (Product # PA5-30428) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-30428
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Full length human Cpn10 Recombinant protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: mouse brain.
Predicted reactivity: Mouse (96%), Rat (98%), Zebrafish (82%), Japanese Medaka (86%), Xenopus laevis (88%), Pig (100%), Chicken (87%), Rhesus Monkey (99%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multiprotein complexes, transportation of nascent polypeptide chains across cellular membranes and regulation of protein folding. Heat shock proteins (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins and the HSP 110 family. The low molecular weight family includes HSP 10, HSP 20, HSP 27 (Heme Oxygenase 1), HSP 32 and HSP 40. Chaperonins are ubiquitous, indispensable proteins that facilitate protein folding in an ATP-dependent manner, enhancing the yield of properly folded substrate protein under conditions where spontaneous folding does not occur. Chaperonins are typified by the E. coli heat-shock proteins GroEL and GroES (HSP 10). HSP 10 is a heptameric ring of identical 10 kDa subunits that binds to each end of GroEL to form a symmetric, functional heterodimer.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 10 kDa chaperonin; 10 kDa heat shock protein, mitochondrial; 10kDa Chaperonin; CH10; Chaperonin 10; Cpn 10; CPN10; early pregnancy factor; Early-pregnancy factor; EPF; heat shock 10 kDa protein 1 (chaperonin 10); heat shock 10kD protein 1 (chaperonin 10); heat shock 10kDa protein 1; Heat shock protein family E member 1; HSP; Hsp10; mitochondrial chaperonin 10
Gene Aliases: 10kDa; CPN10; EPF; GROES; HSP10; HSPE1; mt-cpn10
UniProt ID: (Human) P61604, (Mouse) Q64433
Entrez Gene ID: (Human) 3336, (Mouse) 15528
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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