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Invitrogen
HSPE1 Polyclonal Antibody
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FIGURE: 1 / 16
HSPE1 Antibody (PA5-79415) in ICC/IF
Immunocytochemistry analysis of Cpn10 using anti-Cpn10 antibody (Product # PA5-79415) . Cpn10 was detected in a section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5μg/mL rabbit anti-Cpn10 antibody (Product # PA5-79415) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. ... View More
Product Details
PA5-79415
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Cpn10 (81-102aa VVLDDKDYFLFRDGDILGKYVD).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human A431 whole cell, human A549 whole cell, rat heart tissue, mouse heart tissue. IHC: Human Intestinal Cancer tissue, Mouse Intestine tissue, Rat Intestine tissue, human lung cancer tissue. ICC/IF: HepG2 cell, Hela cell, A549 Cell. Flow: HepG2 cell.
Target Information
The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multiprotein complexes, transportation of nascent polypeptide chains across cellular membranes and regulation of protein folding. Heat shock proteins (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins and the HSP 110 family. The low molecular weight family includes HSP 10, HSP 20, HSP 27 (Heme Oxygenase 1), HSP 32 and HSP 40. Chaperonins are ubiquitous, indispensable proteins that facilitate protein folding in an ATP-dependent manner, enhancing the yield of properly folded substrate protein under conditions where spontaneous folding does not occur. Chaperonins are typified by the E. coli heat-shock proteins GroEL and GroES (HSP 10). HSP 10 is a heptameric ring of identical 10 kDa subunits that binds to each end of GroEL to form a symmetric, functional heterodimer.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 10 kDa chaperonin; 10 kDa heat shock protein, mitochondrial; 10kDa Chaperonin; CH10; Chaperonin 10; Cpn 10; CPN10; early pregnancy factor; Early-pregnancy factor; EPF; Heat shock 10 kD protein 1 (chaperonin 10); heat shock 10 kDa protein 1 (chaperonin 10); heat shock 10kD protein 1 (chaperonin 10); heat shock 10kDa protein 1; Heat shock protein family E member 1; HSP; Hsp10; mitochondrial chaperonin 10
Gene Aliases: 10kDa; CPN10; EPF; GROES; HSP10; HSPE1; mt-cpn10
UniProt ID: (Human) P61604, (Mouse) Q64433
Entrez Gene ID: (Human) 3336, (Rat) 25462, (Mouse) 15528
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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