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HTATSF1 Polyclonal Antibody

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Antibody Testing Data (14)

HTATSF1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

HTATSF1 Antibody (20805-1-AP) in ICC/IF

Immunofluorescent analysis of Hela cells, using HTATSF1 antibody 20805-1-AP at 1:50 dilution and FITC- labeled FITC-Donkey anti-Rabbit IgG (green).

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HTATSF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 20805-1-AP (HTATSF1 antibody) at dilution of 1:100 (under 10x lens).

HTATSF1 Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 20805-1-AP (HTATSF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

HTATSF1 Antibody in Immunoprecipitation (IP) — IP result of anti-HTATSF1 (IP:20805-1-AP, 4ug; Detection:20805-1-AP 1:2000) with HepG2 cells lysate 2400ug.

Product Details

20805-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

HTATSF1 Fusion Protein Ag14744 (1-350 aa encoded by BC009896)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MSGTNLDGN DEFDEQLRMQ ELYGDGKDGD TQTDAGGEPD SLGQQPTDTP YEWDLDKKAW FPKITEDFIA TYQANYGFSN DGASSSTANV EDVHARTAEE PPQEKAPEPT DARKKGEKRK AESGWFHVEE DRNTNVYVSG LPPDITVDEF IQLMSKFGII MRDPQTEEFK VKLYKDNQGN LKGDGLCCYL KRESVELALK LLDEDEIRGY KLHVEVAKFQ LKGEYDASKK KKKCKDYKKK LSMQQKQLDW RPERRAGPSR MRHERVVIIK NMFHPMDFED DPLVLNEIRE DLRVECSKFG QIRKLLLFDR HPDGVASVSF RDPEEADYCI QTLDGRWFGG RQITAQAWDG T (1-350 aa encoded by BC009896)

Target Information

The protein encoded by this gene functions as a cofactor for the stimulation of transcriptional elongation by HIV-1 Tat, which binds to the HIV-1 promoter through Tat-TAR interaction. This protein may also serve as a dual-function factor to couple transcription and splicing and to facilitate their reciprocal activation. Alternatively spliced transcript variants have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A PARylation-phosphorylation cascade promotes TOPBP1 loading and RPA-RAD51 exchange in homologous recombination.

Molecular cell

Zhao J,Tian S,Guo Q,Bao K,Yu G,Wang X,Shen X,Zhang J,Chen J,Yang Y,Liu L,Li X,Hao J,Yang N,Liu Z,Ai D,Yang J,Zhu Y,Yao Z,Ma S,Zhang K,Shi L

Thu Jul 21 00:00:00 EDT 2022

Metabolic protein phosphoglycerate kinase 1 confers lung cancer migration by directly binding HIV Tat specific factor 1.

Cell death discovery

Chang YC,Chan MH,Li CH,Yang CJ,Tseng YW,Tsai HF,Chiou J,Hsiao M

Sat Jun 05 00:00:00 EDT 2021

Metabolic protein phosphoglycerate kinase 1 confers lung cancer migration by directly binding HIV Tat specific factor 1.

Cell death discovery

Chang YC,Chan MH,Li CH,Yang CJ,Tseng YW,Tsai HF,Chiou J,Hsiao M

Sat Jun 05 00:00:00 EDT 2021

Metabolic protein phosphoglycerate kinase 1 confers lung cancer migration by directly binding HIV Tat specific factor 1.

Cell death discovery

Chang YC,Chan MH,Li CH,Yang CJ,Tseng YW,Tsai HF,Chiou J,Hsiao M

Sat Jun 05 00:00:00 EDT 2021

Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.

Nucleic acids research

Lu X,Zhu X,Li Y,Liu M,Yu B,Wang Y,Rao M,Yang H,Zhou K,Wang Y,Chen Y,Chen M,Zhuang S,Chen LF,Liu R,Chen R

Fri Aug 19 00:00:00 EDT 2016

Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.

Nucleic acids research

Lu X,Zhu X,Li Y,Liu M,Yu B,Wang Y,Rao M,Yang H,Zhou K,Wang Y,Chen Y,Chen M,Zhuang S,Chen LF,Liu R,Chen R

Fri Aug 19 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: cofactor required for Tat activation of HIV-1 transcription; HIV TAT specific factor 1; HIV Tat-specific factor 1; HIV Tat-specific factor 1 homolog; RP1-196E23.2; Tat-SF1

Gene Aliases: 1600023H17Rik; 2600017A12Rik; 2700077B20Rik; dJ196E23.2; HTATSF1; RGD1563377; TAT-SF1; TATSF1

UniProt ID: (Human) O43719, (Mouse) Q8BGC0

Entrez Gene ID: (Human) 27336, (Rat) 317612, (Mouse) 72459

Molecular Function: RNA splicing factor RNA metabolism protein RNA processing factor nucleic acid metabolism protein