Invitrogen

HTR4 Polyclonal Antibody

Product Image Gallery

HTR4 Antibody in Western Blot (WB) — Western blot analysis of HTR4 in Lane A: SW480 Whole Cell Lysate (30 µg), Lane B: HeLa Whole Cell Lysate (30 µg). Samples were probed using a HTR4 Polyclonal Antibody (Product # PA5-80698) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), HRP Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:43 kDa. Observed band size:44 kDa.

HTR4 Antibody in Immunoprecipitation (IP) — HTR4 was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate, 4 µL anti-HTR4 rabbit polyclonal antibody and 60 µg of Immunomagnetic beads Protein A/G. Primary antibody: HTR4 Polyclonal Antibody (Product # PA5-80698), at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Immunoprecipitation was performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 43 kDa.

Product Details

Tested Applications

Dilution

Immunoprecipitation (IP)

5-10 µL/mg of lysate

Western Blot (WB)

1:500-1:2000

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to the center region of the Human HTR4/5-HT4

Conjugate

Unconjugated

Form

Liquid

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS

Contains

0.03% Proclin 300

Storage conditions

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

Product Specific Information

This antibody has specificity for Human HTR4/5-HT4.

Target Information

This gene is a member of the family of serotonin receptors, which are G protein coupled receptors that stimulate cAMP production in response to serotonin (5-hydroxytryptamine). The gene product is a glycosylated transmembrane protein that functions in both the peripheral and central nervous system to modulate the release of various neurotransmitters. Multiple transcript variants encoding proteins with distinct C-terminal sequences have been described, but the full-length nature of some transcript variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 5-HT-4; 5-HT4; 5-HT4 receptor; 5-HT4R; 5-hydroxytryptamine (serotonin) receptor 4, G protein-coupled; 5-hydroxytryptamine 4 receptor; 5-hydroxytryptamine receptor 4; 5HT4 receptor; Cardiac 5-HT4 receptor; HTR4; Serotonin 4 receptor; Serotonin 5-HT-4 receptor; Serotonin 5-HT-4a receptor; Serotonin receptor 4

Gene Aliases: 5-HT4; 5-HT4R; HTR4

UniProt ID: (Human) Q13639

Entrez Gene ID: (Human) 3360

Molecular Function: G-protein coupled receptor receptor