Invitrogen

HTR4 Polyclonal Antibody

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HTR4 Antibody in Western Blot (WB) — Western Blot using HTR4 Polyclonal Antibody (Product # PA5-80698) at 1:500 dilution. Lane A: SW480 Whole Cell Lysate, Lane B: HeLa Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 44 kDa.

HTR4 Antibody in Immunoprecipitation (IP) — HTR4 Immunoprecipitation using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 µL with HTR4 Polyclonal Antibody (Product # PA5-80698) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: HTR4 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1,000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 43 kDa.

Product Details

PA5-80698

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunoprecipitation (IP)	5-10 µL/mg of lysate	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthetic peptide corresponding to the center region of the Human HTR4/5-HT4
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A, Antigen affinity chromatography
Storage buffer	PBS, pH 7
Contains	0.03% Proclin 300
Storage conditions	Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
RRID	AB_2787983

Product Specific Information

This antibody has specificity for Human HTR4/5-HT4.

Target Information

This gene is a member of the family of serotonin receptors, which are G protein coupled receptors that stimulate cAMP production in response to serotonin (5-hydroxytryptamine). The gene product is a glycosylated transmembrane protein that functions in both the peripheral and central nervous system to modulate the release of various neurotransmitters. Multiple transcript variants encoding proteins with distinct C-terminal sequences have been described, but the full-length nature of some transcript variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 5-HT-4; 5-HT4 receptor; 5-hydroxytryptamine (serotonin) receptor 4, G protein-coupled; 5-hydroxytryptamine receptor 4; 5HT4; 5HT4 receptor; cardiac 5-HT4 receptor; Serotonin receptor 4

Gene Aliases: 5-HT4; 5-HT4R; HTR4

UniProt ID: (Human) Q13639

Entrez Gene ID: (Human) 3360

Molecular Function: G-protein coupled receptor