Proteintech

HTRA1 Polyclonal Antibody

HTRA1 Antibody in Immunocytochemistry (ICC/IF)

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HTRA1 Antibody (55011-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells using 55011-1-AP (HTRA1 antibody) at dilution of 1:25 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

HTRA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HTRA1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

55011-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

1:10-1:100

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.46 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

The 50 kDa HTRA1 was found at varying levels in the majority of samples tested, whereas the 42 kDa form of HTRA1 was identified in degenerated intervertebral disc protein samples. The antibody is specific to HTRA1.

Target Information

HtrA1, a 51KDa protein, is a member of highly conserved mammalian HtrA serine protease family. It is a human homolog of bacterial HtrA and consists of a secretory signal sequence, an IGFBP-binding domain and a Kazal-type serine-protease inhibitor domain at the N-terminus and a highly conserved trypsin-like serine/protease domain and a PDZ domain at the C-terminus. It plays a major role in the regulation of cell growth. HtrA1 inhibits signaling mediated by TGF-beta family members and regulates many physiological processes. HtrA1 is up-regulated in placenta during pregnancy and plays an important role in implantation and placental physiology. It regulates the availability of IGFs by cleaving IGF-binding proteins. It acts as a tumor suppressor and is down-regulated in ovarian cancers and melanomas. Its role has been implicated in osteoarthritis suggesting a therapeutic intervention. Northern blot analysis detected high expression in placenta.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibiting HIF-1 signaling alleviates HTRA1-induced RPE senescence in retinal degeneration.

Cell communication and signaling : CCS

Xu W,Liu X,Han W,Wu K,Zhao M,Mei T,Shang B,Wu J,Luo J,Lai Y,Yang B,Zhuo Y,Lu L,Liu Y,Tian XL,Zhao L

Wed Jun 14 00:00:00 UTC 2023

Inhibiting HIF-1 signaling alleviates HTRA1-induced RPE senescence in retinal degeneration.

Cell communication and signaling : CCS

Xu W,Liu X,Han W,Wu K,Zhao M,Mei T,Shang B,Wu J,Luo J,Lai Y,Yang B,Zhuo Y,Lu L,Liu Y,Tian XL,Zhao L

Wed Jun 14 00:00:00 UTC 2023

Inhibiting HIF-1 signaling alleviates HTRA1-induced RPE senescence in retinal degeneration.

Cell communication and signaling : CCS

Xu W,Liu X,Han W,Wu K,Zhao M,Mei T,Shang B,Wu J,Luo J,Lai Y,Yang B,Zhuo Y,Lu L,Liu Y,Tian XL,Zhao L

Wed Jun 14 00:00:00 UTC 2023

Inhibiting HIF-1 signaling alleviates HTRA1-induced RPE senescence in retinal degeneration.

Cell communication and signaling : CCS

Xu W,Liu X,Han W,Wu K,Zhao M,Mei T,Shang B,Wu J,Luo J,Lai Y,Yang B,Zhuo Y,Lu L,Liu Y,Tian XL,Zhao L

Wed Jun 14 00:00:00 UTC 2023

Brief exposure to directionally-specific pulsed electromagnetic fields stimulates extracellular vesicle release and is antagonized by streptomycin: A potential regenerative medicine and food industry paradigm.

Biomaterials

Wong CJK,Tai YK,Yap JLY,Fong CHH,Loo LSW,Kukumberg M,Fröhlich J,Zhang S,Li JZ,Wang JW,Rufaihah AJ,Franco-Obregón A

Mon Aug 01 00:00:00 UTC 2022

Long non-coding RNA lincRNA-erythroid prosurvival (EPS) alleviates cerebral ischemia/reperfusion injury by maintaining high-temperature requirement protein A1 (Htra1) stability through recruiting heterogeneous nuclear ribonucleoprotein L (HNRNPL).

Bioengineered

Guo H,Guo X,Jiang S

Sun May 01 00:00:00 UTC 2022

The "Matrisome" reveals the characterization of skin keloid microenvironment.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zhang S,Liu B,Wang W,Lv L,Gao D,Chai M,Li M,Wu Z,Zhu Y,Ma J,Leng L

Thu Apr 01 00:00:00 UTC 2021

The "Matrisome" reveals the characterization of skin keloid microenvironment.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zhang S,Liu B,Wang W,Lv L,Gao D,Chai M,Li M,Wu Z,Zhu Y,Ma J,Leng L

Thu Apr 01 00:00:00 UTC 2021

Murine osteoclasts secrete serine protease HtrA1 capable of degrading osteoprotegerin in the bone microenvironment.

Communications biology

Ochiai N,Nakachi Y,Yokoo T,Ichihara T,Eriksson T,Yonemoto Y,Kato T,Ogata H,Fujimoto N,Kobayashi Y,Udagawa N,Kaku S,Ueki T,Okazaki Y,Takahashi N,Suda T

Thu Apr 16 00:00:00 UTC 2020

High-Temperature Requirement A 1 Causes Photoreceptor Cell Death in Zebrafish Disease Models.

The American journal of pathology

Oura Y,Nakamura M,Takigawa T,Fukushima Y,Wakabayashi T,Tsujikawa M,Nishida K

Sat Dec 01 00:00:00 UTC 2018

High-Temperature Requirement A 1 Causes Photoreceptor Cell Death in Zebrafish Disease Models.

The American journal of pathology

Oura Y,Nakamura M,Takigawa T,Fukushima Y,Wakabayashi T,Tsujikawa M,Nishida K

Sat Dec 01 00:00:00 UTC 2018

High-Temperature Requirement A 1 Causes Photoreceptor Cell Death in Zebrafish Disease Models.

The American journal of pathology

Oura Y,Nakamura M,Takigawa T,Fukushima Y,Wakabayashi T,Tsujikawa M,Nishida K

Sat Dec 01 00:00:00 UTC 2018

High-Temperature Requirement A 1 Causes Photoreceptor Cell Death in Zebrafish Disease Models.

The American journal of pathology

Oura Y,Nakamura M,Takigawa T,Fukushima Y,Wakabayashi T,Tsujikawa M,Nishida K

Sat Dec 01 00:00:00 UTC 2018

Emodin Alleviates Sodium Taurocholate-Induced Pancreatic Acinar Cell Injury via MicroRNA-30a-5p-Mediated Inhibition of High-Temperature Requirement A/Transforming Growth Factor Beta 1 Inflammatory Signaling.

Frontiers in immunology

Xiang H,Tao X,Xia S,Qu J,Song H,Liu J,Shang D

Wed Nov 20 00:00:00 UTC 2019

Frontiers in immunology

Xiang H,Tao X,Xia S,Qu J,Song H,Liu J,Shang D

Wed Nov 20 00:00:00 UTC 2019

Frontiers in immunology

Xiang H,Tao X,Xia S,Qu J,Song H,Liu J,Shang D

Wed Nov 20 00:00:00 UTC 2019

Frontiers in immunology

Xiang H,Tao X,Xia S,Qu J,Song H,Liu J,Shang D

Wed Nov 20 00:00:00 UTC 2019

Proteolytic Degradation and Inflammation Play Critical Roles in Polypoidal Choroidal Vasculopathy.

The American journal of pathology

Kumar S,Nakashizuka H,Jones A,Lambert A,Zhao X,Shen M,Parker M,Wang S,Berriochoa Z,Fnu A,VanBeuge S,Chévez-Barrios P,Tso M,Rainier J,Fu Y

Fri Dec 01 00:00:00 UTC 2017

Proteolytic Degradation and Inflammation Play Critical Roles in Polypoidal Choroidal Vasculopathy.

The American journal of pathology

Kumar S,Nakashizuka H,Jones A,Lambert A,Zhao X,Shen M,Parker M,Wang S,Berriochoa Z,Fnu A,VanBeuge S,Chévez-Barrios P,Tso M,Rainier J,Fu Y

Fri Dec 01 00:00:00 UTC 2017

A frameshift mutation in HTRA1 expands CARASIL syndrome and peripheral small arterial disease to the Chinese population.

Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology

Cai B,Zeng J,Lin Y,Lin Y,Lin W,Lin W,Li Z,Wang N

Sat Aug 01 00:00:00 UTC 2015

A frameshift mutation in HTRA1 expands CARASIL syndrome and peripheral small arterial disease to the Chinese population.

Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology

Cai B,Zeng J,Lin Y,Lin Y,Lin W,Lin W,Li Z,Wang N

Sat Aug 01 00:00:00 UTC 2015

A frameshift mutation in HTRA1 expands CARASIL syndrome and peripheral small arterial disease to the Chinese population.

Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology

Cai B,Zeng J,Lin Y,Lin Y,Lin W,Lin W,Li Z,Wang N

Sat Aug 01 00:00:00 UTC 2015

A frameshift mutation in HTRA1 expands CARASIL syndrome and peripheral small arterial disease to the Chinese population.

Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology

Cai B,Zeng J,Lin Y,Lin Y,Lin W,Lin W,Li Z,Wang N

Sat Aug 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: High-temperature requirement A serine peptidase 1; IGFBP5-protease; insulin-like growth factor binding protein 5 protease; L56; protease serine 11; protease, serine, 11 (IGF binding); Serine protease 11; Serine protease HTRA1

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Gene Aliases: AI429470; ARMD7; CADASIL2; CARASIL; HTRA; HTRA1; L56; ORF480; PRSS11; RSPP11

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UniProt ID: (Human) Q92743, (Rat) Q9QZK5, (Mouse) Q9R118

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Entrez Gene ID: (Human) 5654, (Rat) 65164, (Mouse) 56213

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Function(s)

serine-type endopeptidase activity insulin-like growth factor binding serine-type peptidase activity protein binding peptidase activity hydrolase activity growth factor binding serine protease

Process(es)

regulation of cell growth placenta development proteolysis extracellular matrix disassembly negative regulation of transforming growth factor beta receptor signaling pathway negative regulation of BMP signaling pathway positive regulation of epithelial cell proliferation negative regulation of defense response to virus chorionic trophoblast cell differentiation dentinogenesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HTRA1 Polyclonal Antibody

HTRA1 Antibody (55011-1-AP) in ICC/IF

Product Details

55011-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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