Invitrogen

HYAL1 Polyclonal Antibody

View all (12) HYAL1 antibodies

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Protocols

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HYAL1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of HYAL1 using anti-HYAL1 antibody(Product # PA5-79420).HYAL1 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 1 μg/mL rabbit anti-HYAL1 antibody(Product # PA5-79420)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

HYAL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of HYAL1 in a paraffin-embedded section of Human Intestinal Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution).The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/mL rabbit anti-HYAL1 antibody (Product # PA5-79420) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

HYAL1 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemical analysis of HYAL1 in a frozen section of Human Placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/mL rabbit anti-HYAL1 antibody (Product # PA5-79420) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

HYAL1 Antibody in Western Blot (WB) — Western blot analysis of HYAL1 in, Lane 1: human HepG2 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse kidney tissue lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. The membrane was blocked with 5% non-fat milk/TBS for 1. 5 hour at RT. The membrane was incubated with HYAL1 Polyclonal Antibody (Product # PA5-79420) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0. 1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5,000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit. A specific band was detected for HYAL1 at approximately 60 kDa. The expected band size for HYAL1 is at 48 kDa.

Product Details

PA5-79420

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.5 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

0.5-1 µg/mL

Immunocytochemistry (ICC/IF)

0.5-1 µg/mL

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human HYAL1 (32-47aa FTTVWNANTQWCLERH).

View immunogen

Conjugate

Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5mg BSA

Contains

0.05mg sodium azide, 0.05mg thimerosal

Storage conditions

-20°C

RRID

AB_2746536

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Target Information

This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Hyal-1; Hyaluronidase-1; Hyaluronoglucosaminidase-1; LuCa-1; Lung carcinoma protein 1; plasma hyaluronidase; tumor suppressor LUCA-1

Gene Aliases: HYAL-1; HYAL1; LUCA1; MPS9; NAT6

UniProt ID: (Human) Q12794

Entrez Gene ID: (Human) 3373

Molecular Function: glycosidase

Function(s)

response to reactive oxygen species carbohydrate metabolic process inflammatory response response to virus positive regulation of epithelial cell migration chondroitin sulfate catabolic process hyaluronan metabolic process hyaluronan biosynthetic process hyaluronan catabolic process positive regulation of cell growth negative regulation of cell growth cellular response to platelet-derived growth factor stimulus cellular response to fibroblast growth factor stimulus positive regulation of angiogenesis positive regulation of cell adhesion positive regulation of growth response to antibiotic positive regulation of epithelial cell proliferation cartilage development embryonic skeletal joint morphogenesis cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to pH cellular response to UV-B positive regulation of hyaluranon cable assembly

Process(es)

virus receptor activity hyalurononglucosaminidase activity transcription factor binding hyaluronan synthase activity

HYAL1 Polyclonal Antibody

Product Details

PA5-79420

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

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