Western blot analysis of Haptoglobin using A) 30 µg HeLa whole cell lysate (B) 30 µg HepG2 whole cell lysate and C) 30 µg A375 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Haptoglobin polyclonal antibody (Product # PA5-22255) at a dilution of 1:500.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 129 and 375 of (Uniprot ID#P00738)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-22255 targets Haptoglobin in WB applications and shows reactivity with Human samples.
The PA5-22255 immunogen is a recombinant fragment corresponding to a region within amino acids 129 and 375 of (Uniprot ID#P00738).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.