Invitrogen

Hepsin Polyclonal Antibody

Product Image Gallery

Hepsin Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of Hepsin in MCF-7 cells using a Hepsin polyclonal antibody (Product # PA5-97839) at a dilution of 1:133. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.

Hepsin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Hepsin in paraffin embedded human pancreatic tissue using a Hepsin polyclonal antibody (Product # PA5-97839) at a dilution of 1:400. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Hepsin Antibody in Western Blot (WB) — Western Blot analysis of Hepsin using a Hepsin Polyclonal antibody (Product # PA5-97839) at a concentration of 4.5 µg/mL. Positive WB detected in: MCF-7 whole cell lysate, PC-3 whole cell lysate, Rat kidney tissue, Mouse liver tissue. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 46 kDa.

Product Details

Applications

Tested Dilution

Publications

ELISA (ELISA)

Assay-Dependent

Immunocytochemistry (ICC)

1:50-1:200

Immunofluorescence (IF)

1:50-1:200

Immunohistochemistry (IHC)

1:200-1:500

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Western Blot (WB)

1:500-1:5000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Human Serine protease hepsin protein (294-413AA)

Conjugate

Unconjugated

Form

Liquid

Concentration

2.82 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.03% Proclin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2812453

Target Information

Hepsin is a cell surface serine protease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this antibody

Bioinformatics

Protein Aliases: hepsin (transmembrane protease, serine 1); Serine protease hepsin; Serine protease hepsin catalytic chain; Serine protease hepsin non-catalytic chain; testicular tissue protein Li 85; Transmembrane protease serine 1

Gene Aliases: Hlb320; HPN; TMPRSS1

UniProt ID: (Human) P05981, (Rat) Q05511, (Mouse) O35453

Entrez Gene ID: (Human) 3249, (Rat) 29135, (Mouse) 15451

Molecular Function: annexin calcium-binding protein calmodulin enzyme modulator hydrolase intracellular calcium-sensing protein peptide hormone protease protease inhibitor receptor serine protease signaling molecule

Function(s)

proteolysis regulation of cell shape positive regulation of gene expression negative regulation of epithelial to mesenchymal transition positive regulation of plasminogen activation positive regulation of cell growth basement membrane disassembly negative regulation of apoptotic process positive regulation by host of viral transcription hepatocyte growth factor receptor signaling pathway negative regulation of epithelial cell proliferation detection of mechanical stimulus involved in sensory perception of sound potassium ion transmembrane transport cochlea morphogenesis response to thyroid hormone pilomotor reflex positive regulation of hepatocyte proliferation positive regulation of thyroid hormone generation sensory perception of sound negative regulation of alkaline phosphatase activity cholesterol homeostasis

Process(es)

serine-type endopeptidase activity protein binding peptidase activity serine-type peptidase activity calcium-activated potassium channel activity serine-type exopeptidase activity hydrolase activity

Hepsin Polyclonal Antibody

Product Details

Target Information

References (0)

Bioinformatics

Material safety data sheets (MSDS)

Performance Guarantee

We're here to help

Brands

Shopping Tools

eSolutions

Support

Thermo Fisher Scientific

Thermo Fisher Scientific Inc. announces acquisition of QIAGEN N.V., a leading global provider of molecular diagnostics and sample preparation technologies. Learn more ›

Your items have has been added!