Invitrogen

Hepsin Polyclonal Antibody

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Hepsin Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of Hepsin in MCF-7 cells using a Hepsin polyclonal antibody (Product # PA5-97839) at a dilution of 1:133. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.

Hepsin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Hepsin in paraffin embedded human pancreatic tissue using a Hepsin polyclonal antibody (Product # PA5-97839) at a dilution of 1:400. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Hepsin Antibody in Western Blot (WB) — Western Blot analysis of Hepsin using a Hepsin Polyclonal antibody (Product # PA5-97839) at a concentration of 4.5 µg/mL. Positive WB detected in: MCF-7 whole cell lysate, PC-3 whole cell lysate, Rat kidney tissue, Mouse liver tissue. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 46 kDa.

Product Details

Tested Applications

Dilution

ELISA (ELISA)

Assay-Dependent

Immunocytochemistry (ICC)

1:50-1:200

Immunofluorescence (IF)

1:50-1:200

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Western Blot (WB)

1:500-1:5000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Human Serine protease hepsin protein (294-413AA)

Conjugate

Unconjugated

Form

Liquid

Concentration

2.82 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.03% Proclin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Target Information

This gene encodes a type II transmembrane serine protease. The encoded protein has an extracellular region that consists of two domains, a catalytic serine protease domain and a non-catalytic scavenger receptor cysteine-rich domain. This protein may be involved in diverse cellular functions including blood coagulation, maintenance of cell morphology and the growth and progression of certain cancers, particularly prostate cancer. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: hepsin (transmembrane protease, serine 1); Serine protease hepsin; Serine protease hepsin catalytic chain; Serine protease hepsin non-catalytic chain; testicular tissue protein Li 85; Transmembrane protease serine 1

Gene Aliases: Hlb320; HPN; TMPRSS1

UniProt ID: (Human) P05981, (Rat) Q05511, (Mouse) O35453

Entrez Gene ID: (Human) 3249, (Rat) 29135, (Mouse) 15451

Molecular Function: annexin calcium-binding protein calmodulin enzyme modulator hydrolase intracellular calcium-sensing protein peptide hormone protease protease inhibitor receptor serine protease signaling molecule