Invitrogen

Hepsin Polyclonal Antibody

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Hepsin Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hepsin in MCF-7 cells using a Hepsin polyclonal antibody (Product # PA5-97839) at a dilution of 1:133. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.

Hepsin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Hepsin in paraffin embedded human pancreatic tissue using a Hepsin polyclonal antibody (Product # PA5-97839) at a dilution of 1:400. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Hepsin Antibody in Western Blot (WB) — Western Blot analysis of Hepsin using a Hepsin Polyclonal antibody (Product # PA5-97839) at a concentration of 4.5 µg/mL. Positive WB detected in: MCF-7 whole cell lysate, PC-3 whole cell lysate, Rat kidney tissue, Mouse liver tissue. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 46 kDa.

Product Details

PA5-97839

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:5,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:200-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-
ELISA (ELISA)	Assay-Dependent	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant Human Serine protease hepsin protein (294-413AA) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	2.82 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 50% glycerol
Contains	0.03% Proclin 300
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2812453

Target Information

Hepsin is a cell surface serine protease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: hepsin (transmembrane protease, serine 1); Serine protease hepsin; Serine protease hepsin catalytic chain; Serine protease hepsin non-catalytic chain; testicular tissue protein Li 85; Transmembrane protease serine 1

Gene Aliases: Hlb320; HPN; TMPRSS1

UniProt ID: (Human) P05981, (Rat) Q05511, (Mouse) O35453

Entrez Gene ID: (Human) 3249, (Rat) 29135, (Mouse) 15451

Molecular Function: serine protease