Western blot analysis of HeLa Whole Cell Lysate using PA5-19625, Hex primary antibody at a dilution of 1:250 (lane 1). PA5-19625 staining of HEK 293 Whole Cell Lysate at a dilution of 1:250 (lane 2). Blot treated with a secondary IR Dye680-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:10000.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human Hex.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay-Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:100|
|Immunohistochemistry (PFA fixed) (IHC (PFA))||1:100|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For Western Blot, this antibody has non-specific bands at 42 kDa.
This antibody is predicted to react with rat, human and pig based on sequence homology.
This gene encodes a member of the homeobox family of transcription factors, many of which are involved in developmental processes. Expression in specific hematopoietic lineages suggests that this protein may play a role in hematopoietic differentiation.
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