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Hexokinase 2 Polyclonal Antibody

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Hexokinase 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human skeletal muscle using 22029-1-AP (Hexokinase 2 antibody) at dilution of 1:50 (under 40x lens).

Hexokinase 2 Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22029-1-AP (Hexokinase 2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Hexokinase 2 Antibody in Immunoprecipitation (IP) — IP result of anti-Hexokinase 2 (IP:22029-1-AP, 3ug; Detection:22029-1-AP 1:1000) with HeLa cells lysate 2000ug.

Product Details

22029-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 33 publications 33 publications
Immunohistochemistry (IHC)	-	View 5 publications 5 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Immunoprecipitation (IP)	0.5-4.0 µg	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Pig, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Hexokinase 2 Fusion Protein Ag16895 (568-917 aa encoded by BC021116) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.34 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: HGTGDELFD HIVQCIADFL EYMGMKGVSL PLGFTFSFPC QQNSLDESIL LKWTKGFKAS GCEGEDVVTL LKEAIHRREE FDLDVVAVVN DTVGTMMTCG FEDPHCEVGL IVGTGSNACY MEEMRNVELV EGEEGRMCVN MEWGAFGDNG CLDDFRTEFD VAVDELSLNP GKQRFEKMIS GMYLGEIVRN ILIDFTKRGL LFRGRISERL KTRGIFETKF LSQIESDCLA LLQVRAILQH LGLESTCDDS IIVKEVCTVV ARRAAQLCGA GMAAVVDRIR ENRGLDALKV TVGVDGTLYK LHPHFAKVMH ETVKDLAPKC DVSFLQSEDG SGKGAALITA VACRIREAGQ R (568-917 aa encoded by BC021116)

Target Information

In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV. Hexokinase activity is involved in the first step in several metabolic pathways including phosphorylation of glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P. Hexokinase 2 is the predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DKFZp686M1669; Hexokinase; Hexokinase type II; Hexokinase-2; hexokinase-2 muscle; hexokinase-2, muscle; Hexokinase-B; HK II; Muscle form hexokinase; OTTHUMP00000202738

Gene Aliases: AI642394; HK2; HKII; HXK2

UniProt ID: (Human) P52789, (Mouse) O08528, (Rat) P27881

Entrez Gene ID: (Human) 3099, (Mouse) 15277, (Rat) 25059

Molecular Function: kinase

Function(s)

cellular glucose homeostasis response to ischemia glycolytic process lactation apoptotic mitochondrial changes glucose transport negative regulation of mitochondrial membrane permeability regulation of glucose import carbohydrate phosphorylation glucose 6-phosphate metabolic process canonical glycolysis establishment of protein localization to mitochondrion maintenance of protein location in mitochondrion positive regulation of mitophagy in response to mitochondrial depolarization negative regulation of reactive oxygen species metabolic process carbohydrate metabolic process glucose metabolic process metabolic process phosphorylation glucose catabolic process

Process(es)

glucokinase activity hexokinase activity protein binding ATP binding glucose binding fructokinase activity mannokinase activity nucleotide binding catalytic activity kinase activity transferase activity phosphotransferase activity, alcohol group as acceptor

Hexokinase 2 Polyclonal Antibody

Product Details

22029-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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