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Invitrogen
Histone 1F0 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 9
Histone 1F0 Antibody (PA5-51466) in ICC/IF
Immunofluorescence analysis of Histone 1F0 was performed using 70% confluent log phase U2OS cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Histone 1F0 Rabbit Polyclonal Antibody (Product # PA5-51466) at 5 microgram/mL in 0.1% BSA and incubated overn... View More
Product Details
PA5-51466
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human Histone 1F0. Recombinant protein control fragment (Product #RP-91337).
Conjugate
Form
Liquid
Concentration
0.05 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2642450
Product Specific Information
Immunogen sequence: MTENSTDHPK YSDMIVAAIQ AEKNRAGSSR QSIQKYIKSH YKVGENADSQ IKLSIKRLVT TGVLKQTKGV GASGSFRLAK SDEPKKSVAF KKTKKEIKKV ATPKKASK
Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 39%.
Target Information
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H1 histone family, member 0; H1.0, H1(0), H1-0; Histone H1'; Histone H1(0); Histone H1.0
Gene Aliases: H1-0; H10; H1F0; H1FV
UniProt ID: (Human) P07305
Entrez Gene ID: (Human) 3005
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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