Proteintech

Histone H1.2 Polyclonal Antibody

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Histone H1.2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using HIST1H1C antibody 19649-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Histone H1.2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human ovary tumor using 19649-1-AP (HIST1H1C antibody) at dilution of 1:100 (under 10x lens).

Histone H1.2 Antibody in Western Blot (WB) — Mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19649-1-AP (HIST1H1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Histone H1.2 Antibody in Immunoprecipitation (IP) — IP result of anti-Histone H1.2 (IP:19649-1-AP, 3ug; Detection:19649-1-AP 1:500) with HeLa cells lysate 1600ug.

Product Details

19649-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 20 publications 20 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Histone H1.2 Fusion Protein Ag7503 (1-213 aa encoded by BC002649)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MSETAPAAP AAAPPAEKAP VKKKAAKKAG GTPRKASGPP VSELITKAVA ASKERSGVSL AALKKALAAA GYDVEKNNSR IKLGLKSLVS KGTLVQTKGT GASGSFKLNK KAASGEAKPK VKKAGGTKPK KPVGAAKKPK KAAGGATPKK SAKKTPKKAK KPAAATVTKK VAKSPKKAKV AKPKKAAKSA AKAVKPKAAK PKVVKPKKAA PKKK (1-213 aa encoded by BC002649)

Target Information

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Bioinformatics

Protein Aliases: H1 histone family, member 2; H1 VAR.1; H1c; histone 1, H1c; histone H1; Histone H1.2; Histone H1c; Histone H1d; Histone H1s-1; MGC3992

Gene Aliases: 0610008C09Rik; H1-2; H1.2; H1C; H1F2; H1s-1; H1var1; His1a; HIST1H1C

UniProt ID: (Human) P16403, (Mouse) P15864

Entrez Gene ID: (Human) 3006, (Mouse) 50708

Molecular Function: histone