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Histone H2A.Z Polyclonal Antibody

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Histone H2A.Z Antibody in Western Blot (WB) — Histone H2A.Z antibody tested by Western blot. Detection of H2A.Z by Western blot. The analysis was performed using Histone H2A.Z pAb at a 1:5,000 dilution. Lane 1: 20 ng recombinant histone H2A.Z protein. Lane 2: 5 µg HeLa acid extract. Lane 3: 5 µg HeLa acid extract plus 1 µM immunizing peptide.

Histone H2A.Z Antibody in ChIP assay (ChIP) — Histone H2A.Z antibody tested by ChIP analysis. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 µl of Histone H2A.Z pAb or 10 µl of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

Histone H2A.Z Antibody in ChIP-sequencing (ChIP-Seq) — Histone H2A.Z antibody tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from the human H9 embryonic stem cell line (4.5 million cells) and 5 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 30 million sequence tags were mapped to identify H2A.Z occupancy. H2A.Z is found throughout the genome, is often enriched at promoters and depleted from transcribed genes as shown in the images.

Histone H2A.Z Antibody in Dot blot (DB) — Histone H2A.Z antibody tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H2A.Z antibody for histone H2A.Z. Recombinant proteins corresponding to the immunogen and related proteins were spotted onto PVDF and probed with the antibody at 1:4,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: recombinant Histone H2A. Lane 2: recombinant Histone H2A.Z. Lane 3: recombinant Histone H4.

Product Details

39113

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:5,000	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	-	View 2 publications 2 publications
ChIP assay (ChIP)	2-10 µL	View 1 publication 1 publication
Dot blot (DB)	Assay-dependent	-
ChIP-sequencing (ChIP-Seq)	5 µL	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 6 publications 6 publications

Product Specifications
Species Reactivity	Human
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	This Histone H2A.Z antibody was raised against a peptide derived from the C-terminus of human histone H2A.Z.
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage buffer	whole serum with 30% glycerol
Contains	0.035% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2615081

Target Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Oxoglutarate dehydrogenase and acetyl-CoA acyltransferase 2 selectively associate with H2A.Z-occupied promoters and are required for histone modifications.

Biochimica et biophysica acta. Gene regulatory mechanisms

Choi S,Pfleger J,Jeon YH,Yang Z,He M,Shin H,Sayed D,Astrof S,Abdellatif M

Research topic: Transcription

Tue Oct 01 00:00:00 EDT 2019

BAP1 complex promotes transcription by opposing PRC1-mediated H2A ubiquitylation.

Nature communications

Campagne A,Lee MK,Zielinski D,Michaud A,Le Corre S,Dingli F,Chen H,Shahidian LZ,Vassilev I,Servant N,Loew D,Pasmant E,Postel-Vinay S,Wassef M,Margueron R

Research topic: Transcription

Mon Jan 21 00:00:00 EST 2019

The Histone Chaperone FACT Coordinates H2A.X-Dependent Signaling and Repair of DNA Damage.

Molecular cell

Piquet S,Le Parc F,Bai SK,Chevallier O,Adam S,Polo SE

Research topic: DNA Damage

Thu Dec 06 00:00:00 EST 2018

Histone variants H2A.Z and H3.3 coordinately regulate PRC2-dependent H3K27me3 deposition and gene expression regulation in mES cells.

BMC biology

Wang Y,Long H,Yu J,Dong L,Wassef M,Zhuo B,Li X,Zhao J,Wang M,Liu C,Wen Z,Chang L,Chen P,Wang QF,Xu X,Margueron R,Li G

Research topic: Gene Regulation

Mon Sep 24 00:00:00 EDT 2018

PARP1-dependent recruitment of the FBXL10-RNF68-RNF2 ubiquitin ligase to sites of DNA damage controls H2A.Z loading.

eLife

Rona G,Roberti D,Yin Y,Pagan JK,Homer H,Sassani E,Zeke A,Busino L,Rothenberg E,Pagano M

Research topic: DNA Damage

Mon Jul 09 00:00:00 EDT 2018

Transcriptional regulation mediated by H2A.Z via ANP32e-dependent inhibition of protein phosphatase 2A.

Biochimica et biophysica acta. Gene regulatory mechanisms

Shin H,He M,Yang Z,Jeon YH,Pfleger J,Sayed D,Abdellatif M

Research topic: Cardiovascular

Tue May 01 00:00:00 EDT 2018

Expression of Non-acetylatable H2A.Z in Myoblast Cells Blocks Myoblast Differentiation through Disruption of MyoD Expression.

The Journal of biological chemistry

Law C,Cheung P

Research topic: Cell Differentiation

Fri May 22 00:00:00 EDT 2015

Expression of Non-acetylatable H2A.Z in Myoblast Cells Blocks Myoblast Differentiation through Disruption of MyoD Expression.

The Journal of biological chemistry

Law C,Cheung P

Research topic: Cell Differentiation

Fri May 22 00:00:00 EDT 2015

Expression of Non-acetylatable H2A.Z in Myoblast Cells Blocks Myoblast Differentiation through Disruption of MyoD Expression.

The Journal of biological chemistry

Law C,Cheung P

Research topic: Cell Differentiation

Fri May 22 00:00:00 EDT 2015

The histone variant composition of centromeres is controlled by the pericentric heterochromatin state during the cell cycle.

Journal of cell science

Boyarchuk E,Filipescu D,Vassias I,Cantaloube S,Almouzni G

Research topic: Cell Cycle, Centromeres

Fri Aug 01 00:00:00 EDT 2014

CLOCK:BMAL1 is a pioneer-like transcription factor.

Genes & development

Menet JS,Pescatore S,Rosbash M

Research topic: Circadian Clock

Wed Jan 01 00:00:00 EST 2014

Bioinformatics

Protein Aliases: 11ac; H2A histone family, member V; H2A histone family, member Z; H2A.F/Z; H2A.Z variant histone 2; H2A.ZK5; H2A.ZK5ac; H2A/z; H2AFZac; H2AZ histone; H2AZK11ac; H2AZK7ac; histone H2A.F/Z; Histone H2A.V; Histone H2A.Z; purine-rich binding element protein B

Gene Aliases: H2A.z; H2A.Z-1; H2A.Z-2; H2A/z; H2AFV; H2AFZ; H2AV; H2AZ; H2AZ1; H2AZ2

UniProt ID: (Human) Q71UI9, (Human) P0C0S5

Entrez Gene ID: (Human) 94239, (Human) 3015

Molecular Function: histone