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Histone H3.3K27M Polyclonal Antibody
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FIGURE: 1 / 2
Histone H3.3K27M Antibody (61803) in WB
Western blot analysis of Hantibody specificity for Histone H3.3K27M 0.1 µg per lane of Recombinant Human Histone H3.3 Cat. No. 31295 (Lanes 1 & 3) or Recombinant Human Histone H3.3K27M Cat. No. 31552 (Lanes 2 & 4) were probed with AbFlex® Histone H3.3 antibody (rAb), Cat. No. 91191 at 2 µg/mL (Lanes 1 & 2) or Histone H3.3K27M antibody (pAb) at 0.05 µg/mL.
Product Details
61803
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This antibody was generated using a peptide containing a methionine substitution at lysine 27 of human Histone H3.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Purification
Antigen affinity chromatography, Affinity chromatography
Storage buffer
PBS, pH 7.5, with 30% glycerol
Contains
0.035% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H3 histone; H3 histone family member 3A; H3 histone family member 3B; H3 histone, family 3A; H3 histone, family 3B (H3.3B); H3 K27; H3 K27M; H3.3 histone; H3/b; H3/d; H3/f; H3/j; H3a; H3K27 mutant; H3K27M; Histone 3; Histone H3; Histone H3 K27M; Histone H3.3; Histone H3K27M; unnamed protein product
Gene Aliases: BRYLIB1; BRYLIB2; H3-3A; H3-3B; H3.3A; H3.3B; H3F3; H3F3A; H3F3B; PP781
UniProt ID: (Human) P84243
Entrez Gene ID: (Human) 3020, (Human) 3021
oocyte maturation
osteoblast differentiation
nucleosome assembly
nucleus organization
spermatogenesis
spermatid development
single fertilization
embryo implantation
cell proliferation
male gonad development
positive regulation of cell growth
pericentric heterochromatin assembly
telomeric heterochromatin assembly
telomere organization
multicellular organism growth
muscle cell differentiation
oogenesis
regulation of centromere complex assembly
negative regulation of chromosome condensation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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