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Histone extracts of HeLa cells (15 µg) were analyzed by western blot using the anti-H3R17me2 crude serum (Cat. no. 49-1021), diluted 1:250 in TBS-Tween containing 5% skimmed milk. The location of the protein of interest is indicated with an arrow; the marker (in kDa) is shown on the right.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Raised against histone H3 containing the dimethylated arginine 17 (H3R17me2), using a KLH-conjugated synthetic peptide.|
|Storage buffer||whole serum|
|Contains||<0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||10-15 ug|
|ELISA (ELISA)||1:1,000 to 1:3,000|
|Western Blot (WB)||1:250|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post tranlationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
H3 histone family, member A; H3a; H3R17me2; HIST1H3A; Histone 1; histone 1, H3a; histone H3/a
H3/A; H3FA; HIST1H3A