Western blot analysis of Calf Thymus Histone lysate using PA5-19475, Histone H4 primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19475 staining of HeLa Histone Lysate at a dilution of 1 ug/ml (lane 2). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.
|Tested species reactivity||Bovine, Human, Yeast|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant full length protein of Saccharomyces cerevisiae Histone H4 expressed in E. coli|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-19475 targets Histone H4 in Western blot and flow cytometry applications and shows reactivity with Bovine, Human, and Yeast samples.
The PA5-19475 immunogen is recombinant full length protein of Saccharomyces cerevisiae Histone H4 expressed in E. coli.
PA5-19475 detects Histone H4 which has a predicted molecular weight of approximately 14 kDa.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.