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Invitrogen
Hopx Polyclonal Antibody
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FIGURE: 1 / 4
Hopx Antibody (PA5-90538) in WB
Western blot analysis of Hopx in extracts of various cell lines. Samples were incubated with Hopx Polyclonal antibody (Product # PA5-90538) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Product Details
PA5-90538
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein (or fragment).
Conjugate
Form
Liquid
Concentration
2.61 mg/mL
Amount
261 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MSAETASGPT EDQVEILEYN FNKVDKHPDS TTLCLIAAEA GLSEEETQKW FKQRLAKWRR SEGLPSECRS VTD; Positive Samples: mouse brain, mouse lung, rat lung; Cellular Location: Nucleus
Target Information
HOPX is an atypical homeodomain protein which does not bind DNA and is required to modulate cardiac growth and development. HOPX acts via its interaction with SRF, thereby modulating the expression of SRF-dependent cardiac-specific genes and cardiac development. HOPX prevents SRF-dependent transcription either by inhibiting SRF binding to DNA or by recruiting histone deacetylase (HDAC) proteins that prevent transcription by SRF. HOPX overexpression causes cardiac hypertrophy and may act as a tumor suppressor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: GIIg15b; global ischemia induced protein GIIG15B; Global ischemia-induced gene 15B protein; global ischemia-induced protein 15B; homeobox domain; homeobox only domain; Homeobox-only protein; homeodomain only protein; Homeodomain-only protein; Lung cancer-associated Y protein; mOB1; not expressed in choriocarcinoma clone 1; Not expressed in choriocarcinoma protein 1; odd homeobox 1; Odd homeobox protein 1; unnamed protein product
Gene Aliases: 1110018K11Rik; 1200015P04Rik; 2300002F06Rik; AI848177; AW490897; CAMEO; GIIg15b; Hdop; HOD; HOP; HOPX; LAGY; NECC1; OB1; Obl; SMAP31; TOTO
UniProt ID: (Human) Q9BPY8, (Mouse) Q8R1H0, (Rat) Q78ZR5
Entrez Gene ID: (Human) 84525, (Mouse) 74318, (Rat) 171160
negative regulation of transcription from RNA polymerase II promoter
trophectodermal cell differentiation
transcription, DNA-templated
regulation of transcription, DNA-templated
multicellular organism development
heart development
regulation of heart contraction
histone deacetylation
regulation of protein binding
positive regulation of skeletal muscle tissue regeneration
negative regulation of cell differentiation
lung alveolus development
positive regulation of striated muscle cell differentiation
bundle of His development
regulation of transcription from RNA polymerase II promoter
cell differentiation
chaperone-mediated protein complex assembly
positive regulation of gap junction assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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