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Invitrogen
HuD Polyclonal Antibody
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FIGURE: 1 / 8
HuD Antibody (PA5-79199) in IHC (P)
Immunohistochemical analysis of ELAVL4 in paraffin-embedded section of human glioma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-ELAVL4 antibody (Product # PA5-79199) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Ra... View More
Product Details
PA5-79199
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human ELAVL4 (8-45aa MEPQVSNGPTSNTSNGPSSNNRNCPSPMQTGATTDDSK).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat brain tissue, mouse brain tissue, U87-MG whole cell. IHC: mouse brain tissue, rat brain tissue, human meningeoma tissue.
Target Information
HuD, otherwise known as ELAV-like protein 4 or PNEM, is a protein that in humans is encoded by the ELAVL4 gene. This human gene is located at 1p34 by in situ hybridization. The HuD/ELAVL4 protein is an RNA-binding protein. ELAVL4 has specificity for 3-prime uridylate-rich untranslated regions of growth factor mRNAs. HuD is expressed only in neurons and it binds to AU-rich element-containing mRNAs. As a result of this interaction the, half-life of the transcript is increased. Additionally, HuD is important in neurons during brain development and plasticity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D); ELAV like neuron-specific RNA binding protein 4; ELAV-like protein 4; Hu antigen D; HU-antigen D; HuD; paraneoplastic encephalomyelitis antigen; Paraneoplastic encephalomyelitis antigen HuD; RNA-binding protein HUD3; unnamed protein product
Gene Aliases: Elav; ELAVL4; HUD; PNEM; r-HuD; RGD1561943
UniProt ID: (Human) P26378, (Mouse) Q61701, (Rat) O09032
Entrez Gene ID: (Human) 1996, (Mouse) 15572, (Rat) 432358
RNA processing
mRNA processing
learning
locomotory behavior
RNA splicing
cerebral cortex neuron differentiation
dendrite morphogenesis
3'-UTR-mediated mRNA stabilization
positive regulation of 3'-UTR-mediated mRNA stabilization
neuron differentiation
regulation of mRNA stability
positive regulation of dendrite development
nervous system development
aging
associative learning
regeneration
response to endoplasmic reticulum stress
response to cocaine
cellular response to nerve growth factor stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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