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Product Details
PHG9014
| Product Specifications | |
|---|---|
Species |
Human, Mouse, Rat |
Expression System |
Insect |
Amino acid sequence |
Human Activin A recombinant protein is a homodimer consisting of two disulfide-linked 116 amino acid subunits |
Molecular weight |
25 kDa |
Class |
Recombinant |
Type |
Protein |
Purity |
>95% by SDS-PAGE |
Endotoxin concentration |
<1 EU/mL |
Activity |
ED50 ≤ 2 ng/mL; determined by the dose-dependent inhibition of mouse MPC-11 cells. |
Conjugate |
Unconjugated |
Form |
Lyophilized |
Purification |
sequential chromatography |
Storage buffer |
PBS |
Contains |
no preservative |
Storage conditions |
-20°C |
Product Specific Information
Carrier-Free
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute lyophilized recombinant human Activin A in sterile PBS containing 0.1% human serum albumin. Further dilutions should be made in low endotoxin medium or a buffered solution containing a carrier protein such as heat inactivated FCS or tissue culture grade BSA.
Storage: Store this lyophilized preparation at < -20°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at < -20°C. Avoid repeated freeze/thaw cycles.
Target Information
Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I ( I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Activin A mutations are associated with fibrodysplasia ossificans progressive.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: activin A; Activin beta-A chain; EDF; erythroid differentiation factor; Erythroid differentiation protein; follicle-stimulating hormone-releasing protein; FSH-releasing protein; Inhibin beta A chain; inhibin, beta A (activin A, activin AB alpha polypeptide); Inhibin, beta-1
Gene Aliases: EDF; FRP; INHBA
UniProt ID: (Human) P08476, (Rat) P18331, (Mouse) Q04998
Entrez Gene ID: (Human) 3624, (Rat) 29200, (Mouse) 16323
Molecular Function:
growth factor
G1/S transition of mitotic cell cycle
ovarian follicle development
hair follicle development
hematopoietic progenitor cell differentiation
regulation of transcription from RNA polymerase II promoter
defense response
cell cycle arrest
cell surface receptor signaling pathway
cell-cell signaling
nervous system development
negative regulation of cell proliferation
male gonad development
positive regulation of gene expression
positive regulation of pathway-restricted SMAD protein phosphorylation
striatal medium spiny neuron differentiation
cell differentiation
erythrocyte differentiation
negative regulation of cell growth
positive regulation of cellular protein metabolic process
activin receptor signaling pathway
endodermal cell differentiation
growth
negative regulation of phosphorylation
odontogenesis
response to drug
hemoglobin biosynthetic process
progesterone secretion
regulation of MAPK cascade
negative regulation of interferon-gamma biosynthetic process
negative regulation of B cell differentiation
positive regulation of erythrocyte differentiation
negative regulation of macrophage differentiation
negative regulation of cell cycle
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of follicle-stimulating hormone secretion
positive regulation of follicle-stimulating hormone secretion
negative regulation of follicle-stimulating hormone secretion
mesodermal cell differentiation
cell development
palate development
positive regulation of ovulation
SMAD protein signal transduction
eyelid development in camera-type eye
cellular response to follicle-stimulating hormone stimulus
cellular response to cholesterol
GABAergic neuron differentiation
extrinsic apoptotic signaling pathway
positive regulation of extrinsic apoptotic signaling pathway in absence of ligand
mesoderm formation
regulation of apoptotic process
negative regulation of hair follicle development
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