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Gibco

Human FGF-9 Recombinant Protein

View all Human FGF-9 Proteins
Datasheet
Tech Support
Datasheet
Tech Support

Product Details

PHG0194

Applications Tested Dilution Publications

Western Blot (WB)

 Assay-dependent -

Immunohistochemistry (IHC)

 Assay-dependent -

ELISA (ELISA)

 Assay-dependent -

Functional Assay (FN)

 Assay-dependent -

Control (Ctrl)

 Assay-dependent -
Product Specifications

Species

 Human

Expression System

 E. coli

Amino acid sequence

 Human FGF-9 recombinant protein contains 206 amino acids

Molecular weight

 23.4 kDa

Class

 Recombinant

Type

 Protein

Purity

 ≥95% by SDS-PAGE and HPLC

Endotoxin concentration

 <0.1 ng/µg

Activity

 ED50 ≤ 10 ng/mL; determined by the dose-dependent proliferation of Balb/c 3T3 cells.

Conjugate

 Unconjugated

Form

 Lyophilized

Purification

 sequential chromatography

Contains

 no preservative

Storage conditions

 -20°C

Product Specific Information

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This lyophilized protein should be reconstituted in sterile, distilled water to a concentration of 0.1-1 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA, and store in working aliquots at -20°C to -80°C.

Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage, preferably desiccated. Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.

Target Information

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FGF; FGF-9; Fibroblast growth factor; Fibroblast growth factor 9; fibroblast growth factor 9 (glia-activating factor); GAF; Glia-activating factor; HBGF-9; Heparin-binding growth factor 9; M-FGF-9

Gene Aliases: FGF-9; FGF9; GAF; HBFG-9; HBGF-9; SYNS3

UniProt ID: (Human) P31371

Entrez Gene ID: (Human) 2254

Molecular Function: growth factor intercellular signal molecule

Function(s)
negative regulation of transcription from RNA polymerase II promoter MAPK cascade angiogenesis osteoblast differentiation eye development positive regulation of mesenchymal cell proliferation chondrocyte differentiation protein import into nucleus signal transduction cell-cell signaling positive regulation of cell proliferation fibroblast growth factor receptor signaling pathway male gonad development positive regulation of gene expression regulation of phosphatidylinositol 3-kinase signaling peptidyl-tyrosine phosphorylation substantia nigra development negative regulation of Wnt signaling pathway male sex determination embryonic limb morphogenesis positive regulation of vascular endothelial growth factor receptor signaling pathway positive regulation of activin receptor signaling pathway phosphatidylinositol-3-phosphate biosynthetic process inner ear morphogenesis positive regulation of MAPK cascade positive regulation of GTPase activity positive regulation of smoothened signaling pathway phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling regulation of timing of cell differentiation embryonic digestive tract development embryonic skeletal system development positive regulation of epithelial cell proliferation positive regulation of cell division positive regulation of cardiac muscle cell proliferation lung-associated mesenchyme development positive regulation of canonical Wnt signaling pathway
Process(es)
protein tyrosine kinase activity Ras guanyl-nucleotide exchange factor activity fibroblast growth factor receptor binding growth factor activity heparin binding 1-phosphatidylinositol-3-kinase activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity
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