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Product Details
PHC7084
| Product Specifications | |
|---|---|
Species |
Human |
Expression System |
E. coli |
Amino acid sequence |
Human Midkine recombinant protein contains 123 amino acids |
Molecular weight |
13.4 kDa |
Class |
Recombinant |
Type |
Protein |
Purity |
>98% by SDS-PAGE |
Endotoxin concentration |
<0.1 ng/µg |
Activity |
ED50 = 0.1 - 10 ng/mL; determined by the dose-dependent chemotaxis of human neutrophils. |
Conjugate |
Unconjugated |
Form |
Lyophilized |
Purification |
sequential chromatography |
Storage buffer |
PBS |
Contains |
no preservative |
Storage conditions |
-20°C |
Product Specific Information
Carrier-Free
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This lyophilized protein should be reconstituted in sterile, distilled water to a concentration of 0.1-1 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C.
Target Information
Midkine is a retinoic acid-responsive, heparin-binding growth factor expressed in various cell types during embryogenesis. It promotes angiogenesis, cell growth, and cell migration. Midkine is also expressed in several carcinomas, suggesting that it may play a role in tumorigenesis, perhaps through its effects on angiogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Amphiregulin-associated protein; ARAP; FLJ27379; Midgestation and kidney protein; Midkine; MK; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein; retinoic acid inducible factor
Gene Aliases: ARAP; MDK; MK; MK1; NEGF2
UniProt ID: (Human) P21741
Entrez Gene ID: (Human) 4192
Molecular Function:
growth factor
behavioral fear response
signal transduction
Notch signaling pathway
nervous system development
short-term memory
response to wounding
cell migration
dentate gyrus development
cerebellar granular layer development
cerebral cortex development
cell differentiation
adrenal gland development
defecation
response to drug
negative regulation of neuron apoptotic process
positive regulation of transcription, DNA-templated
regulation of behavior
response to glucocorticoid
positive regulation of cell division
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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