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Product Details
PHC1615
| Product Specifications | |
|---|---|
Species |
Human |
Expression System |
E. coli |
Amino acid sequence |
Human alpha-Defensin 1 recombinant protein contains 30 amino acids |
Molecular weight |
3.4 kDa |
Class |
Recombinant |
Type |
Protein |
Purity |
≥98% by SDS-PAGE and HPLC |
Endotoxin concentration |
<0.1 ng/µg |
Activity |
ED50 = 1 - 10 ng/mL; determined by the dose-dependent chemotaxis of immature dendritic cells. |
Conjugate |
Unconjugated |
Form |
Lyophilized |
Purification |
sequential chromatography |
Contains |
no preservative |
Storage conditions |
-20°C |
Product Specific Information
Carrier-Free
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute NP-1 in sterile, distilled water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C.
Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage. Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Target Information
Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Defensin, alpha 1; defensin, alpha 1, myeloid-related sequence; defensin, alpha 2; HNP-1; HNP-2; HP 1-56; HP-1; HP-2; myeloid-related sequence; Neutrophil defensin 1; Neutrophil defensin 2
Gene Aliases: DEF1; DEFA1; DEFA1B; DEFA2; HNP-1; HP-1; HP1; MRS
UniProt ID: (Human) P59665
Entrez Gene ID: (Human) 1667
Molecular Function:
antimicrobial response protein
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