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Bethyl Laboratories
Huntingtin Polyclonal Antibody
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FIGURE: 1 / 1
Huntingtin Antibody (A302-812A) in WB
Detection of human Huntingtin by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Huntingtin antibody (Product # A302-812A) used for WB at 0.04 µg/mL (A) and 0.4 µg/mL (B) and used for IP at 3 µg/mg lysate. Huntingtin was also immunoprecipitated by rabbit anti-Huntingtin antibody A302-813A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 secon... View More
Product Details
A302-812A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 1150 and 1200 of human Huntingtin.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Amount
20 µg
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Huntingtin is a disease gene linked to Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. HD is a mid-life onset autosomal dominant neurodegenerative disease that is characterized by psychiatric disorders, dementia, and involuntary movements (chorea), leading to death in 10-20 years.The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HD protein; Huntingtin; Huntington disease protein; unnamed protein product
Gene Aliases: HD; HTT; IT15; LOMARS
UniProt ID: (Human) P42858
Entrez Gene ID: (Human) 3064
establishment of mitotic spindle orientation
retrograde vesicle-mediated transport, Golgi to ER
apoptotic process
microtubule-based process
Golgi organization
central nervous system development
regulation of signal transduction
neurogenesis
positive regulation of inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity
protein destabilization
vocal learning
positive regulation of apoptotic process
negative regulation of apoptotic process
positive regulation of cilium assembly
vesicle transport along microtubule
synaptic vesicle transport
positive regulation of mitophagy
positive regulation of lipophagy
regulation of CAMKK-AMPK signaling cascade
positive regulation of CAMKK-AMPK signaling cascade
positive regulation of aggrephagy
negative regulation of extrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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