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IARS Antibody (A304-748A-T) in WB
Detection of human and mouse IARS by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-IARS antibody A304-748A-M (lot A304-748A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A304-748A-T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Between 1212 and 1262
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
IARS is a member of aminoacyl-tRNA synthetases which catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Two alternatively spliced variants have been isolated that represent alternate 5' UTRs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ20736; ileRS; IRS; isoleucine tRNA ligase 1, cytoplasmic; Isoleucine--tRNA ligase, cytoplasmic; Isoleucyl-tRNA synthetase; isoleucyl-tRNA synthetase, cytoplasmic
Gene Aliases: 2510016L12Rik; AI327140; AU044614; E430001P04Rik; IARS; IARS1; ILERS; ILRS; IRS; PRO0785
UniProt ID: (Human) P41252, (Mouse) Q8BU30
Entrez Gene ID: (Human) 3376, (Mouse) 105148
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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