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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • IBA1 Antibodies

          Invitrogen

          IBA1 Polyclonal Antibody

          9 Published Figures
          19 References
          View all (54) IBA1 antibodies

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          Datasheet
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          Datasheet
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          Cite IBA1 Polyclonal Antibody

          • Antibody Testing Data (3)
          • Published Figures (9)
          IBA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          IBA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 12

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          IBA1 Antibody (PA5-18039) in IHC (P)

          Immunohistochemistry (PFA fixed) analysis of IBA1 using IBA1 Polyclonal Antibody (Product # PA5-18039) (6 µg/mL) in staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          IBA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          IBA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          IBA1 Antibody in Western Blot (WB)
          IBA1 Antibody in Immunocytochemistry (ICC/IF)
          IBA1 Antibody in Immunohistochemistry (IHC)
          IBA1 Antibody in Immunohistochemistry (IHC)
          IBA1 Antibody in Immunohistochemistry (IHC)
          IBA1 Antibody in Immunohistochemistry (IHC)
          IBA1 Antibody in Immunohistochemistry (IHC)
          IBA1 Antibody in Immunohistochemistry (IHC)
          IBA1 Antibody in Immunohistochemistry (IHC)
          IBA1 Antibody in Western Blot (WB)

          Product Details

          PA5-18039

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.3-1 µg/mL
          View 1 publication 1 publication

          Immunohistochemistry (IHC)

          -
          View 12 publications 12 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          6-8 µg/mL
          View 1 publication 1 publication

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 2 publications 2 publications

          Immunocytochemistry (ICC/IF)

          -
          View 2 publications 2 publications

          Miscellaneous PubMed (Misc)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human, Mouse, Pig, Rat

          Host/Isotype

          Goat / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide sequence (TGPPAKKAISELP) corresponding to the C-terminus amino acids of AIF1 (aa 135-147).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Ammonium sulfate precipitation

          Storage buffer

          TBS, pH 7.3, with 0.5% BSA

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10982846

          Product Specific Information

          This antibody is predicted to react with human and porcine based on sequence homology.

          This antibody is tested in Peptide ELISA: antibody detection limit dilution 64,000.

          Target Information

          Ionized calcium-binding adapter molecule 1 (IBA1), also known by its gene name AIF1, is a protein expressed predominantly by microglia in the brain and spinal cord. This protein belongs to the EF-hand calcium-binding protein family and plays a crucial role in microglial activation and migration in response to brain injury or neuroinflammation. IBA1's function is integral to microglial motility and phagocytic activity, facilitating the cellular response to pathogenic stimuli and promoting tissue homeostasis and repair in the central nervous system. IBA1 serves as a reliable marker for activated microglia in various neurological disorders, including Alzheimer's disease, Parkinson's disease, and multiple sclerosis, where increased expression correlates with disease progression and severity. The protein's structural features enable it to bind calcium ions, inducing conformational changes that activate signaling pathways essential for microglial function. Its expression is highly regulated by inflammatory cytokines, underpinning its role in neuroimmune responses. Due to its specific expression in microglia during pathological conditions, IBA1 is widely used in research as a marker to study microglial status and activity, and it remains a focal point for understanding microglial involvement in neurodegenerative diseases.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: AIF-1; Allograft inflammatory factor 1; balloon angioplasty responsive transcript; balloon angioplasty-responsive transcript 1; DADB-70P7.8; DASS-82G15.2; Em:AF129756.17; Iba; interferon gamma responsive transcript; ionized calcium binding adapter molecule 1; Ionized calcium-binding adapter molecule 1; Microglia response factor; MRF-1; protein BART-1; Protein G1; testis specific

          View more View less

          Gene Aliases: AI607846; AIF-1; AIF1; BART-1; Bart1; D17H6S50E; G1; IBA1; IRT-1; IRT1; mrf-1; Mrf1

          View more View less

          UniProt ID: (Human) P55008, (Mouse) O70200, (Rat) P55009

          View more View less

          Entrez Gene ID: (Human) 199, (Mouse) 11629, (Rat) 29427

          View more View less

          Function(s)
          molecular_function calcium ion binding actin filament binding actin binding protein binding metal ion binding calmodulin-related
          Process(es)
          microglial cell activation positive regulation of protein phosphorylation phagocytosis, engulfment inflammatory response negative regulation of gene expression positive regulation of muscle hyperplasia Rac protein signal transduction actin filament polymerization cellular response to extracellular stimulus cellular response to hormone stimulus positive regulation of T cell proliferation negative regulation of apoptotic process positive regulation of nitric oxide biosynthetic process positive regulation of smooth muscle cell proliferation negative regulation of smooth muscle cell proliferation response to axon injury actin filament bundle assembly response to glucocorticoid response to electrical stimulus cellular response to morphine cellular response to interferon-gamma cellular response to hydroperoxide negative regulation of smooth muscle cell chemotaxis positive regulation of smooth muscle cell chemotaxis positive regulation of monocyte chemotaxis ruffle assembly positive regulation of G1/S transition of mitotic cell cycle positive regulation of T cell migration positive regulation of cell migration response to cytokine macrophage activation
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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