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IBA1 Polyclonal Antibody

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IBA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of IBA1 in Human Placenta using a IBA1 monoclonal antibody (Product #PA5-18039) at 5 µg/mL. The Human Placenta tissue section was paraffin embeded and detected using steamed antigen retrieval with citrate buffer pH 6, AP-staining.

IBA1 Antibody in Western Blot (WB) — Western blot analysis of AIF1/IBA1 in Human Frontal Cortex (A) and Rat Brain (B) lysate (35µg protein in RIPA buffer). Samples were probed with the AIF1/IBA1 antibody (Product # PA5-18039, 0.3µg/mL) for 1 hour. Western blot was detected by chemiluminescence.

IBA1 Antibody in Immunocytochemistry (ICC/IF) — Figure 6. A , B , Microglia density in the inner ( A ) and outer ( B ) dentate, as measured by the mean (+-SEM) percentage area covered by Iba1 + stain throughout the whole hippocampus and in the dorsal and ventral subregions. C , D , Phagocytic activity in the inner ( C ) and outer ( D ) dentate, as measured by the mean (+-SEM) percentage area covered by CD68 + stain throughout the whole hippocampus and in the dorsal and ventral subregions. E , F , Relative degree of microglial phagocytic activity in the inner ( E ) and outer ( F ) dentate, as measured by the mean (+-SEM) percentage of Iba1 + area also positive for CD68 throughout the whole hippocampus and in the dorsal and ventral subregions. G , H , Absolute microglial phagocytic activity in the inner ( G ) and outer ( H ) dentate, as measured by the mean (+-SEM) percentage area positive for colocalized Iba1 and CD68 throughout the whole hippocampus, and in the dorsal and ventral subregions. N = 10/group. * p < 0.05, ** p < 0.01, *** p < 0.001. I , Representative images of Iba1 + (green) and CD68 + (red) cells in the dorsal dentate gyrus. DAPI (blue) counterstaining was used to visualize nuclei, determine position relative to bregma, and create ROIs around the dentate gyrus. J , Representative image of inner and outer ROIs of the dentate gyrus. See Figure 6-1 for estimation statistics performed for microglia-related measures.

IBA1 Antibody in Immunohistochemistry (IHC) — Figure 5 Microglia and astrocyte changes in the cortex and hippocampus of control (n = 5), KD (n = 5), mTBI (n = 5), and mTBI + KD (n = 5) mice. KD reduces the mTBI-induced elevation in active astrocyte expression in the DGH only and not in activated microglia expression at 30 days post-injury. Graphs present quantification of the total surface labeled with ( A ) GFAP in the DGH ( B ) GFAP in the temporal cortex ( C ) Iba-1 in the DGH ( D ) Iba-1 in the temporal cortex. In addition, ( E , F ) Astrocyte morphology in the DG Hilus region displayed approximately twice the ramification as the control samples. ( G ) Representative images of immunohistochemical staining in the DGH and the temporal cortex are presented. GFAP positive cells are shown in green, Iba-1 positive cells are shown in yellow, and NeuN+ cells in red. The scale bars are 20-25 mum.

Product Details

PA5-18039

Applications	Tested Dilution	Publications
Western Blot (WB)	0.2-0.5 µg/mL	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 7 publications 7 publications
Immunohistochemistry (Paraffin) (IHC (P))	4 µg/mL	View 1 publication 1 publication
Immunohistochemistry (Frozen) (IHC (F))	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	-	View 3 publications 3 publications
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse, Pig, Rat
Host/Isotype	Goat / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide sequence (TGPPAKKAISELP) corresponding to the C-terminus amino acids of AIF1
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	TBS, pH 7.3, with 0.5% BSA
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_10982846

Product Specific Information

This antibody is predicted to react with human and porcine based on sequence homology.

Target Information

AIF1 is induced by cytokines and interferon. It is thought to be involved in the negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Ketogenic Diet as a potential treatment for traumatic brain injury in mice.

Scientific reports

Har-Even M,Rubovitch V,Ratliff WA,Richmond-Hacham B,Citron BA,Pick CG

PA5-18039 was used in Immunohistochemistry to support accumulating evidence that KD may be an effective approach to increase the brain's resistance to damage and suggest a potential new therapeutic strategy for treating TBI.

Tue Dec 07 00:00:00 EST 2021

Ketogenic Diet as a potential treatment for traumatic brain injury in mice.

Scientific reports

Har-Even M,Rubovitch V,Ratliff WA,Richmond-Hacham B,Citron BA,Pick CG

Tue Dec 07 00:00:00 EST 2021

High-Fat Diet-Induced Obesity Causes Sex-Specific Deficits in Adult Hippocampal Neurogenesis in Mice.

eNeuro

Robison LS,Albert NM,Camargo LA,Anderson BM,Salinero AE,Riccio DA,Abi-Ghanem C,Gannon OJ,Zuloaga KL

Published figure using IBA1 polyclonal antibody (Product # PA5-18039) in Immunocytochemistry

Fri Jun 18 00:00:00 EDT 2021

Lytic Cell Death in Specific Microglial Subsets Is Required for Preventing Atypical Behavior in Mice.

eNeuro

Chuang HC,Nichols EK,Rauch I,Chang WC,Lin PM,Misra R,Kitaoka M,Vance RE,Saijo K

PA5-18039 was used in Immunocytochemistry to show that mice deficient in inflammasome regulators, including caspase-1 (Casp1), NLR family pyrin domain containing 3 (Nlrp3), IL-1 receptor (Il-1r), and gasdermin D (Gsdmd), exhibit behavior abnormalities characterized by hyperactivity and low anxiety levels.

Fri Jun 18 00:00:00 EDT 2021

Lytic Cell Death in Specific Microglial Subsets Is Required for Preventing Atypical Behavior in Mice.

eNeuro

Chuang HC,Nichols EK,Rauch I,Chang WC,Lin PM,Misra R,Kitaoka M,Vance RE,Saijo K

Fri Jun 18 00:00:00 EDT 2021

Microglial neuropilin-1 promotes oligodendrocyte expansion during development and remyelination by trans-activating platelet-derived growth factor receptor.

Nature communications

Sherafat A,Pfeiffer F,Reiss AM,Wood WM,Nishiyama A

PA5-18039 was used in Immunohistochemistry to determine the mechanism underlying the differential response of oligodendrocyte precursor cells in gray and white matter to platelet-derived growth factor AA, exploring the role of type 1 transmembrane molecule Nrp1.

Thu Apr 15 00:00:00 EDT 2021

Efficient control of Japanese encephalitis virus in the central nervous system of infected pigs occurs in the absence of a pronounced inflammatory immune response.

Journal of neuroinflammation

Redant V,Favoreel HW,Dallmeier K,Van Campe W,De Regge N

PA5-18039 was used in Immunohistochemistry (Paraffin) to show that the NLRP3 inflammasome, a key mediator of neurologic disease in mice, is not upregulated in pigs, further supporting its important role in JEV infections.

Fri Oct 23 00:00:00 EDT 2020

Efficient control of Japanese encephalitis virus in the central nervous system of infected pigs occurs in the absence of a pronounced inflammatory immune response.

Journal of neuroinflammation

Redant V,Favoreel HW,Dallmeier K,Van Campe W,De Regge N

Fri Oct 23 00:00:00 EDT 2020

Nicotinic Acetylcholine Receptor α9 and α10 Subunits Are Expressed in the Brain of Mice.

Frontiers in cellular neuroscience

Lykhmus O,Voytenko LP,Lips KS,Bergen I,Krasteva-Christ G,Vetter DE,Kummer W,Skok M

PA5-18039 was used in Immunohistochemistry to conclude that α9 and α10 nAChR subunits are expressed in distinct neurons of the mouse brain and in the brain mitochondria and are compensatory up-regulated in the absence of α7 subunits.

Wed Sep 30 00:00:00 EDT 2020

Nicotinic Acetylcholine Receptor α9 and α10 Subunits Are Expressed in the Brain of Mice.

Frontiers in cellular neuroscience

Lykhmus O,Voytenko LP,Lips KS,Bergen I,Krasteva-Christ G,Vetter DE,Kummer W,Skok M

Wed Sep 30 00:00:00 EDT 2020

Role of sex and high-fat diet in metabolic and hypothalamic disturbances in the 3xTg-AD mouse model of Alzheimer's disease.

Journal of neuroinflammation

Robison LS,Gannon OJ,Thomas MA,Salinero AE,Abi-Ghanem C,Poitelon Y,Belin S,Zuloaga KL

PA5-18039 was used in Immunocytochemistry to provide further evidence for the roles of hypothalamic and metabolic dysfunction in AD, which in the 3xTg-AD mouse model appears to be dependent on both sex and diet.

Tue Sep 29 00:00:00 EDT 2020

Involvement of homodomain interacting protein kinase 2-c-Jun N-terminal kinase/c-Jun cascade in the long-term synaptic toxicity and cognition impairment induced by neonatal Sevoflurane exposure.

Journal of neurochemistry

Liang L,Xie R,Lu R,Ma R,Wang X,Wang F,Liu B,Wu S,Wang Y,Zhang H

PA5-18039 was used in Immunohistochemistry to reveal a novel role of HIPK2-JNK/c-Jun signaling in the long-term synaptic toxicity and cognition impairment of neonatal Sevoflurane exposure.

Sat Aug 01 00:00:00 EDT 2020

The effect of rosmarinic acid on deformities occurring in brain tissue by craniectomy method. Histopathological evaluation of IBA-1 and GFAP expressions.

Acta cirurgica brasileira

Özevren H,Deveci E,Tuncer MC

PA5-18039 was used in Immunohistochemistry-immunofluorescence to investigate the role of Rosmarinic acid (RA) in the prevention of traumatic brain injury and the immunohistochemical analysis of IBA-1 and GFAP expressions.

Mon Jun 29 00:00:00 EDT 2020

The effect of rosmarinic acid on deformities occurring in brain tissue by craniectomy method. Histopathological evaluation of IBA-1 and GFAP expressions.

Acta cirurgica brasileira

Özevren H,Deveci E,Tuncer MC

Mon Jun 29 00:00:00 EDT 2020

A Single Primary Blast-Induced Traumatic Brain Injury in a Rodent Model Causes Cell-Type Dependent Increase in Nicotinamide Adenine Dinucleotide Phosphate Oxidase Isoforms in Vulnerable Brain Regions.

Journal of neurotrauma

Rama Rao KV,Iring S,Younger D,Kuriakose M,Skotak M,Alay E,Gupta RK,Chandra N

PA5-18039 was used in Immunofluorescence in immunohistochemistry on frozen tissues to examine the expression of NOX1 and NOX2, the corresponding superoxide production, and the extent of lipid peroxidation adducts of 4-hydroxynonenal to a range of proteins following traumatic brain injury.

Sat Sep 01 00:00:00 EDT 2018

Ablation of CCAAT/Enhancer-Binding Protein Delta (C/EBPD): Increased Plaque Burden in a Murine Alzheimer's Disease Model.

PloS one

Lutzenberger M,Burwinkel M,Riemer C,Bode V,Baier M

PA5-18039 was used in western blot to investigate the role of CCAAT/enhancer-binding protein delta in murine models of prion and Alzheimer's disease

Mon May 02 00:00:00 EDT 2016

Ablation of CCAAT/Enhancer-Binding Protein Delta (C/EBPD): Increased Plaque Burden in a Murine Alzheimer's Disease Model.

PloS one

Lutzenberger M,Burwinkel M,Riemer C,Bode V,Baier M

PA5-18039 was used in western blot to investigate the role of CCAAT/enhancer-binding protein delta in murine models of prion and Alzheimer's disease

Mon May 02 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: AIF-1; Allograft inflammatory factor 1; balloon angioplasty responsive transcript; balloon angioplasty-responsive transcript 1; DADB-70P7.8; DASS-82G15.2; Em:AF129756.17; Iba; interferon gamma responsive transcript; ionized calcium binding adapter molecule 1; Ionized calcium-binding adapter molecule 1; Microglia response factor; MRF-1; protein BART-1; Protein G1; testis specific

Gene Aliases: AI607846; AIF-1; AIF1; BART-1; Bart1; D17H6S50E; G1; IBA1; IRT-1; IRT1; mrf-1; Mrf1

UniProt ID: (Human) P55008, (Mouse) O70200, (Rat) P55009

Entrez Gene ID: (Human) 199, (Mouse) 11629, (Rat) 29427

Molecular Function: calmodulin-related

Function(s)

Process(es)

molecular_function calcium ion binding actin filament binding actin binding protein binding metal ion binding

IBA1 Polyclonal Antibody

Product Details

PA5-18039

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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