Invitrogen

IBA1 Polyclonal Antibody

9 Published Figures

19 References

View all (54) IBA1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Published Figures (9)

IBA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 12

IBA1 Antibody (PA5-18039) in IHC (P)

Immunohistochemistry (PFA fixed) analysis of IBA1 using IBA1 Polyclonal Antibody (Product # PA5-18039) (6 µg/mL) in staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

IBA1 Antibody in Immunocytochemistry (ICC/IF)

IBA1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

PA5-18039

Applications

Tested Dilution

Publications

Western Blot (WB)

0.3-1 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 12 publications 12 publications

Immunohistochemistry (Paraffin) (IHC (P))

6-8 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Pig, Rat

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (TGPPAKKAISELP) corresponding to the C-terminus amino acids of AIF1 (aa 135-147).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10982846

Product Specific Information

This antibody is predicted to react with human and porcine based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 64,000.

Target Information

Ionized calcium-binding adapter molecule 1 (IBA1), also known by its gene name AIF1, is a protein expressed predominantly by microglia in the brain and spinal cord. This protein belongs to the EF-hand calcium-binding protein family and plays a crucial role in microglial activation and migration in response to brain injury or neuroinflammation. IBA1's function is integral to microglial motility and phagocytic activity, facilitating the cellular response to pathogenic stimuli and promoting tissue homeostasis and repair in the central nervous system. IBA1 serves as a reliable marker for activated microglia in various neurological disorders, including Alzheimer's disease, Parkinson's disease, and multiple sclerosis, where increased expression correlates with disease progression and severity. The protein's structural features enable it to bind calcium ions, inducing conformational changes that activate signaling pathways essential for microglial function. Its expression is highly regulated by inflammatory cytokines, underpinning its role in neuroimmune responses. Due to its specific expression in microglia during pathological conditions, IBA1 is widely used in research as a marker to study microglial status and activity, and it remains a focal point for understanding microglial involvement in neurodegenerative diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Sex differences in the effects of high fat diet on underlying neuropathology in a mouse model of VCID.

Biology of sex differences

Abi-Ghanem C,Salinero AE,Kordit D,Mansour FM,Kelly RD,Venkataganesh H,Kyaw NR,Gannon OJ,Riccio D,Fredman G,Poitelon Y,Belin S,Kopec AM,Robison LS,Zuloaga KL

PA5-18039 was used in Immunohistochemistry to report on sex differences in the underlying neuropathology, specifically white matter changes and neuroinflammation in several areas of the brain.

Fri May 19 00:00:00 UTC 2023

An updated suite of viral vectors for in vivo calcium imaging using intracerebral and retro-orbital injections in male mice.

Nature communications

Grødem S,Nymoen I,Vatne GH,Rogge FS,Björnsdóttir V,Lensjø KK,Fyhn M

PA5-18039 was used in Immunohistochemistry (Paraffin), Western Blot to explore the use of systemically administered PHP.eB AAVs for brain-wide expression of GECIs and show that systemic administration is a promising strategy for imaging neural activity.

Sat Feb 04 00:00:00 UTC 2023

Effect of minocycline and its nano-formulation on central auditory system in blast-induced hearing loss rat model.

Journal of otology

Perumal V,Ravula AR,Shao N,Chandra N

PA5-18039 was used in Immunohistochemistry (Paraffin), Western Blot to demonstrate that in moderate blast induced hearing loss, minocycline and its nanoparticle formulation exhibited the optimal therapeutic effect on the recovery of the ABR impairment and a protective effect through central auditory system.

Sun Jan 01 00:00:00 UTC 2023

Brain atlas for glycoprotein hormone receptors at single-transcript level.

eLife

Ryu V,Gumerova A,Korkmaz F,Kang SS,Katsel P,Miyashita S,Kannangara H,Cullen L,Chan P,Kuo T,Padilla A,Sultana F,Wizman SA,Kramskiy N,Zaidi S,Kim SM,New MI,Rosen CJ,Goosens KA,Frolinger T,Haroutunian V,Ye K,Lizneva D,Davies TF,Yuen T,Zaidi M

PA5-18039 was used in Immunohistochemistry to create a comprehensive neuroanatomical atlas on the expression of TSHR, LHCGR, and FSHR.

Fri Sep 02 00:00:00 UTC 2022

FSH blockade improves cognition in mice with Alzheimer's disease.

Nature

Xiong J,Kang SS,Wang Z,Liu X,Kuo TC,Korkmaz F,Padilla A,Miyashita S,Chan P,Zhang Z,Katsel P,Burgess J,Gumerova A,Ievleva K,Sant D,Yu SP,Muradova V,Frolinger T,Lizneva D,Iqbal J,Goosens KA,Gera S,Rosen CJ,Haroutunian V,Ryu V,Yuen T,Zaidi M,Ye K

PA5-18039 was used in Immunohistochemistry-immunofluorescence to suggest a causal role for rising serum FSH levels in the exaggerated Alzheimer's disease pathophysiology during menopause, but also reveal an opportunity for treating Alzheimer's disease, obesity, osteoporosis and dyslipidaemia with a single FSH-blocking agent.

Tue Mar 01 00:00:00 UTC 2022

Lytic Cell Death in Specific Microglial Subsets Is Required for Preventing Atypical Behavior in Mice.

eNeuro

Chuang HC,Nichols EK,Rauch I,Chang WC,Lin PM,Misra R,Kitaoka M,Vance RE,Saijo K

PA5-18039 was used in Immunocytochemistry to show that mice deficient in inflammasome regulators, including caspase-1 (Casp1), NLR family pyrin domain containing 3 (Nlrp3), IL-1 receptor (Il-1r), and gasdermin D (Gsdmd), exhibit behavior abnormalities characterized by hyperactivity and low anxiety levels.

Fri Jun 18 00:00:00 UTC 2021

Lytic Cell Death in Specific Microglial Subsets Is Required for Preventing Atypical Behavior in Mice.

eNeuro

Chuang HC,Nichols EK,Rauch I,Chang WC,Lin PM,Misra R,Kitaoka M,Vance RE,Saijo K

Fri Jun 18 00:00:00 UTC 2021

Ketogenic Diet as a potential treatment for traumatic brain injury in mice.

Scientific reports

Har-Even M,Rubovitch V,Ratliff WA,Richmond-Hacham B,Citron BA,Pick CG

Published figure using IBA1 polyclonal antibody (Product # PA5-18039) in Immunohistochemistry

Tue Dec 07 00:00:00 UTC 2021

Microglial neuropilin-1 promotes oligodendrocyte expansion during development and remyelination by trans-activating platelet-derived growth factor receptor.

Nature communications

Sherafat A,Pfeiffer F,Reiss AM,Wood WM,Nishiyama A

PA5-18039 was used in Immunohistochemistry to determine the mechanism underlying the differential response of oligodendrocyte precursor cells in gray and white matter to platelet-derived growth factor AA, exploring the role of type 1 transmembrane molecule Nrp1.

Thu Apr 15 00:00:00 UTC 2021

Involvement of homodomain interacting protein kinase 2-c-Jun N-terminal kinase/c-Jun cascade in the long-term synaptic toxicity and cognition impairment induced by neonatal Sevoflurane exposure.

Journal of neurochemistry

Liang L,Xie R,Lu R,Ma R,Wang X,Wang F,Liu B,Wu S,Wang Y,Zhang H

PA5-18039 was used in Immunohistochemistry to reveal a novel role of HIPK2-JNK/c-Jun signaling in the long-term synaptic toxicity and cognition impairment of neonatal Sevoflurane exposure.

Sat Aug 01 00:00:00 UTC 2020

The effect of rosmarinic acid on deformities occurring in brain tissue by craniectomy method. Histopathological evaluation of IBA-1 and GFAP expressions.

Acta cirurgica brasileira

Özevren H,Deveci E,Tuncer MC

Published figure using IBA1 polyclonal antibody (Product # PA5-18039) in Immunohistochemistry

Mon Jun 29 00:00:00 UTC 2020

The effect of rosmarinic acid on deformities occurring in brain tissue by craniectomy method. Histopathological evaluation of IBA-1 and GFAP expressions.

Acta cirurgica brasileira

Özevren H,Deveci E,Tuncer MC

Published figure using IBA1 polyclonal antibody (Product # PA5-18039) in Immunohistochemistry

Mon Jun 29 00:00:00 UTC 2020

Efficient control of Japanese encephalitis virus in the central nervous system of infected pigs occurs in the absence of a pronounced inflammatory immune response.

Journal of neuroinflammation

Redant V,Favoreel HW,Dallmeier K,Van Campe W,De Regge N

Published figure using IBA1 polyclonal antibody (Product # PA5-18039) in Immunohistochemistry

Fri Oct 23 00:00:00 UTC 2020

Efficient control of Japanese encephalitis virus in the central nervous system of infected pigs occurs in the absence of a pronounced inflammatory immune response.

Journal of neuroinflammation

Redant V,Favoreel HW,Dallmeier K,Van Campe W,De Regge N

Published figure using IBA1 polyclonal antibody (Product # PA5-18039) in Immunohistochemistry

Fri Oct 23 00:00:00 UTC 2020

High-Fat Diet-Induced Obesity Causes Sex-Specific Deficits in Adult Hippocampal Neurogenesis in Mice.

eNeuro

Robison LS,Albert NM,Camargo LA,Anderson BM,Salinero AE,Riccio DA,Abi-Ghanem C,Gannon OJ,Zuloaga KL

Published figure using IBA1 polyclonal antibody (Product # PA5-18039) in Immunocytochemistry

Fri Jun 18 00:00:00 UTC 2021

Role of sex and high-fat diet in metabolic and hypothalamic disturbances in the 3xTg-AD mouse model of Alzheimer's disease.

Journal of neuroinflammation

Robison LS,Gannon OJ,Thomas MA,Salinero AE,Abi-Ghanem C,Poitelon Y,Belin S,Zuloaga KL

Published figure using IBA1 polyclonal antibody (Product # PA5-18039) in Immunohistochemistry (Frozen)

Tue Sep 29 00:00:00 UTC 2020

Role of sex and high-fat diet in metabolic and hypothalamic disturbances in the 3xTg-AD mouse model of Alzheimer's disease.

Journal of neuroinflammation

Robison LS,Gannon OJ,Thomas MA,Salinero AE,Abi-Ghanem C,Poitelon Y,Belin S,Zuloaga KL

Published figure using IBA1 polyclonal antibody (Product # PA5-18039) in Immunohistochemistry (Frozen)

Tue Sep 29 00:00:00 UTC 2020

A Single Primary Blast-Induced Traumatic Brain Injury in a Rodent Model Causes Cell-Type Dependent Increase in Nicotinamide Adenine Dinucleotide Phosphate Oxidase Isoforms in Vulnerable Brain Regions.

Journal of neurotrauma

Rama Rao KV,Iring S,Younger D,Kuriakose M,Skotak M,Alay E,Gupta RK,Chandra N

PA5-18039 was used in Immunofluorescence in immunohistochemistry on frozen tissues to examine the expression of NOX1 and NOX2, the corresponding superoxide production, and the extent of lipid peroxidation adducts of 4-hydroxynonenal to a range of proteins following traumatic brain injury.

Sat Sep 01 00:00:00 UTC 2018

Nicotinic Acetylcholine Receptor α9 and α10 Subunits Are Expressed in the Brain of Mice.

Frontiers in cellular neuroscience

Lykhmus O,Voytenko LP,Lips KS,Bergen I,Krasteva-Christ G,Vetter DE,Kummer W,Skok M

PA5-18039 was used in Immunohistochemistry to conclude that α9 and α10 nAChR subunits are expressed in distinct neurons of the mouse brain and in the brain mitochondria and are compensatory up-regulated in the absence of α7 subunits.

Wed Sep 30 00:00:00 UTC 2020

Nicotinic Acetylcholine Receptor α9 and α10 Subunits Are Expressed in the Brain of Mice.

Frontiers in cellular neuroscience

Lykhmus O,Voytenko LP,Lips KS,Bergen I,Krasteva-Christ G,Vetter DE,Kummer W,Skok M

Wed Sep 30 00:00:00 UTC 2020

Ablation of CCAAT/Enhancer-Binding Protein Delta (C/EBPD): Increased Plaque Burden in a Murine Alzheimer's Disease Model.

PloS one

Lutzenberger M,Burwinkel M,Riemer C,Bode V,Baier M

Published figure using IBA1 polyclonal antibody (Product # PA5-18039) in Western Blot

Mon May 02 00:00:00 UTC 2016

Ablation of CCAAT/Enhancer-Binding Protein Delta (C/EBPD): Increased Plaque Burden in a Murine Alzheimer's Disease Model.

PloS one

Lutzenberger M,Burwinkel M,Riemer C,Bode V,Baier M

Published figure using IBA1 polyclonal antibody (Product # PA5-18039) in Western Blot

Mon May 02 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: AIF-1; Allograft inflammatory factor 1; balloon angioplasty responsive transcript; balloon angioplasty-responsive transcript 1; DADB-70P7.8; DASS-82G15.2; Em:AF129756.17; Iba; interferon gamma responsive transcript; ionized calcium binding adapter molecule 1; Ionized calcium-binding adapter molecule 1; Microglia response factor; MRF-1; protein BART-1; Protein G1; testis specific

View more View less

Gene Aliases: AI607846; AIF-1; AIF1; BART-1; Bart1; D17H6S50E; G1; IBA1; IRT-1; IRT1; mrf-1; Mrf1

View more View less

UniProt ID: (Human) P55008, (Mouse) O70200, (Rat) P55009

View more View less

Entrez Gene ID: (Human) 199, (Mouse) 11629, (Rat) 29427

View more View less

Function(s)

molecular_function calcium ion binding actin filament binding actin binding protein binding metal ion binding calmodulin-related

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

IBA1 Polyclonal Antibody

IBA1 Antibody (PA5-18039) in IHC (P)

Product Details

PA5-18039

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!