Invitrogen

IBA1 Polyclonal Antibody

1 Published Figure

2 References

View all (15) IBA1 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

IBA1 Antibody — Published figure using IBA1 polyclonal antibody (Product # PA5-18039)

IBA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of AIF1/IBA1 in paraffin embedded Mouse Brain. Steamed antigen retrieval was performed with Tris/EDTA buffer pH 9, followed by the application of the AIF1/IBA1 antibody (Product # PA5-18039) (2µg/mL) and HRP-staining.

IBA1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of IBA1 in Human Placenta using a IBA1 monoclonal antibody (Product #PA5-18039) at 5 µg/mL. The Human Placenta tissue section was paraffin embeded and detected using steamed antigen retrieval with citrate buffer pH 6, AP-staining.

IBA1 Antibody in Western Blot (WB) — Western blot analysis of AIF1/IBA1 in Human Frontal Cortex (A) and Rat Brain (B) lysate (35µg protein in RIPA buffer). Samples were probed with the AIF1/IBA1 antibody (Product # PA5-18039, 0.3µg/mL) for 1 hour. Western blot was detected by chemiluminescence.

Product Details

Tested Applications

Dilution

Immunohistochemistry (Paraffin) (IHC (P))

2-4 µg/mL

Western Blot (WB)

0.3-1 µg/mL

Published Applications

Miscellaneous PubMed (Misc)

See 1 publication below

Western Blot (WB)

See 1 publication below

Product Specifications

Species Reactivity

Mouse, Pig, Rat

Published species

Mouse

Host / Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (TGPPAKKAISELP) corresponding to the C-terminus amino acids of AIF1

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_10982846

Product Specific Information

This antibody is predicted to react with human and porcine based on sequence homology.

Target Information

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: AIF-1; AIF1; Allograft inflammatory factor 1; balloon angioplasty responsive transcript; balloon angioplasty-responsive transcript 1; DADB-70P7.8, Iba; Daintain; IBA1; interferon gamma responsive transcript; ionized calcium binding adapter molecule 1; ionized calcium-binding adapter molecule; Ionized calcium-binding adapter molecule 1; IRT-1; Microglia response factor; MRF-1; protein BART-1; Protein G1; testis specific

Gene Aliases: AI607846; AIF-1; AIF1; BART-1; Bart1; D17H6S50E; G1; IBA1; IRT-1; IRT1; mrf-1; Mrf1

UniProt ID: (Human) P55008, (Mouse) O70200, (Rat) P55009

Entrez Gene ID: (Human) 199, (Mouse) 11629, (Rat) 29427

Molecular Function: annexin calcium-binding protein calmodulin intracellular calcium-sensing protein