Invitrogen

ICAM-1 Polyclonal Antibody

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ICAM-1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of ICAM-1 (CD54) was performed in PC12 cells using ICAM-1 Polyclonal Antibody (Product # PA5-96365).

ICAM-1 Antibody in Western Blot (WB) — Western Blot analysis of ICAM-1 (CD54) in extracts of various cell lines using ICAM-1 Polyclonal Antibody (Product # PA5-96365) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Product Details

PA5-96365

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunocytochemistry (ICC/IF)	1:50 - 1:200	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 250-480 of human ICAM1. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.444 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2808167

Product Specific Information

Immunogen sequence: VHLALGDQRL NPTVTYGNDS FSAKASVSVT AEDEGTQRLT CAVILGNQSQ ETLQTVTIYS FPAPNVILTK PEVSEGTEVT VKCEAHPRAK VTLNGVPAQP LGPRAQLLLK ATPEDNGRSF SCSATLEVAG QLIHKNQTRE LRVLYGPRLD ERDCPGNWTW PENSQQTPMC QAWGNPLPEL KCLKDGTFPL PIGESVTVTR DLEGTYLCRA RSTQGEVTRK VTVNVLSPRY E; Positive Samples: Raji, Mouse lung, Rat lung; Cellular Location: Membrane, Single-pass type I membrane protein

Target Information

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

An in vitro model of antibody-mediated injury to glomerular endothelial cells: Upregulation of MHC class II and adhesion molecules.

Transplant immunology

Wilson NA,Dylewski J,Degner KR,O'Neill MA,Reese SR,Hidalgo LG,Blaine J,Panzer SE

PA5-96365 was used in Immunocytochemistry to study whether the MHC class II ligation on glomerular endothelial cells (GEnC) would result in upregulation of adhesion molecules and production of chemoattractants.

Sat Feb 01 00:00:00 EST 2020

Bioinformatics

Protein Aliases: CD54; cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; Ly 47; Major group rhinovirus receptor; MALA-2; MALA2; MyD10; sCD54; soluble CD54; Surface antigen of activated B cells

Gene Aliases: BB2; CD54; ICAM; Icam-1; ICAM1; Ly-47; MALA-2; P3.58

UniProt ID: (Human) P05362, (Mouse) P13597, (Rat) Q00238

Entrez Gene ID: (Human) 3383, (Mouse) 15894, (Rat) 25464

Function(s)