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ID1 Polyclonal Antibody

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Antibody Testing Data (3)

ID1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 3

ID1 Antibody (18475-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed PC-3 cells using 18475-1-AP (ID1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

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Product Details

18475-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:500

View 4 publications 4 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ID1 Fusion Protein Ag13359 (1-155 aa encoded by BC000613)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.41 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MKVASGSTA TAAAGPSCAL KAGKTASGAG EVVRCLSEQS VAISRCAGGA GARLPALLDE QQVNVLLYDM NGCYSRLKEL VPTLPQNRKV SKVEILQHVI DYIRDLQLEL NSESEVGTPG GRGLPVRAPL STLNGEISAL TAEAACVPAD DRILCR (1-155 aa encoded by B C000613)

Target Information

ID1 is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. It has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene.The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Palovarotene Can Attenuate Heterotopic Ossification Induced by Tendon Stem Cells by Downregulating the Synergistic Effects of Smad and NF-κB Signaling Pathway following Stimulation of the Inflammatory Microenvironment.

Stem cells international

Huang J,Lin J,Li C,Tang B,Xiao H

Sat Jul 16 00:00:00 EDT 2022

Stem cells international

Huang J,Lin J,Li C,Tang B,Xiao H

Sat Jul 16 00:00:00 EDT 2022

Stem cells international

Huang J,Lin J,Li C,Tang B,Xiao H

Sat Jul 16 00:00:00 EDT 2022

Functions of retinal astrocytes and Müller cells in mammalian myopia.

BMC ophthalmology

Zhang X,Yu X,Wen Y,Jin L,Zhang L,Zhu H,Zhang D,Xie C,Guo D,Tong J,Shen Y

Thu Nov 24 00:00:00 EST 2022

Ursodesoxycholic acid alleviates liver fibrosis via proregeneration by activation of the ID1-WNT2/HGF signaling pathway.

Clinical and translational medicine

Dong X,Luo Y,Lu S,Ma H,Zhang W,Zhu Y,Sun G,Sun X

Mon Feb 01 00:00:00 EST 2021

Pantoprazole ameliorates liver fibrosis and suppresses hepatic stellate cell activation in bile duct ligation rats by promoting YAP degradation.

Acta pharmacologica Sinica

Lu ZN,Niu WX,Zhang N,Ge MX,Bao YY,Ren Y,Guo XL,He HW

Mon Nov 01 00:00:00 EDT 2021

Endothelial progenitor cells as critical mediators of environmental air pollution-induced cardiovascular toxicity.

American journal of physiology. Heart and circulatory physiology

Singh P,O'Toole TE,Conklin DJ,Hill BG,Haberzettl P

Thu Apr 01 00:00:00 EDT 2021

The miR-182/Myadm axis regulates hypoxia-induced pulmonary hypertension by balancing the BMP- and TGF-β-signalling pathways in an SMC/EC-crosstalk-associated manner.

Basic research in cardiology

Bai Y,Wang J,Chen Y,Lv T,Wang X,Liu C,Xue H,He K,Sun L

Tue Sep 21 00:00:00 EDT 2021

IL-1β Promotes Stemness of Tumor Cells by Activating Smad/ID1 Signaling Pathway.

International journal of medical sciences

Lu L,Wang P,Zou Y,Zha Z,Huang H,Guan M,Wu Y,Liu G

Mon Mar 08 00:00:00 EST 2021

Exosomal miR-125a-5p derived from silica-exposed macrophages induces fibroblast transdifferentiation.

Ecotoxicology and environmental safety

Wang D,Hao C,Zhang L,Zhang J,Liu S,Li Y,Qu Y,Zhao Y,Huang R,Wei J,Yao W

Wed Apr 01 00:00:00 EDT 2020

Defining multistep cell fate decision pathways during pancreatic development at single-cell resolution.

The EMBO journal

Yu XX,Qiu WL,Yang L,Zhang Y,He MY,Li LC,Xu CR

Mon Apr 15 00:00:00 EDT 2019

Expansion and maintenance of primary corneal epithelial stem/progenitor cells by inhibition of TGFβ receptor I-mediated signaling.

Experimental eye research

Hu L,Pu Q,Zhang Y,Ma Q,Li G,Li X

Wed May 01 00:00:00 EDT 2019

Id1/NR2B Receptor Pathway Regulates Rat Cochlear Sensory Epithelial Cell Survival after Radiation.

Audiology & neuro-otology

Chen J,Zhou X,Zou T,Wang B,Yu Y,Lin F,Chen K,Lai Y,Sun K

Mon Oct 14 00:00:00 EDT 2019

Id1/NR2B Receptor Pathway Regulates Rat Cochlear Sensory Epithelial Cell Survival after Radiation.

Audiology & neuro-otology

Chen J,Zhou X,Zou T,Wang B,Yu Y,Lin F,Chen K,Lai Y,Sun K

Mon Oct 14 00:00:00 EDT 2019

High extracellular pressure promotes gastric cancer cell adhesion, invasion, migration and suppresses gastric cancer cell differentiation.

Oncology reports

Su C,Zhang B,Liu W,Zheng H,Sun L,Tong J,Wang T,Jiang X,Liang H,Xue L,Zhang Q

Mon Aug 01 00:00:00 EDT 2016

High extracellular pressure promotes gastric cancer cell adhesion, invasion, migration and suppresses gastric cancer cell differentiation.

Oncology reports

Su C,Zhang B,Liu W,Zheng H,Sun L,Tong J,Wang T,Jiang X,Liang H,Xue L,Zhang Q

Mon Aug 01 00:00:00 EDT 2016

Bone morphogenetic protein 4, inhibitor of differentiation 1, and epidermal growth factor receptor regulate the survival of cochlear sensory epithelial cells.

Journal of neuroscience research

Chen J,Sun W,Zheng Y,Xiong H,Cai Y

Mon Apr 01 00:00:00 EDT 2013

Bone morphogenetic protein 4, inhibitor of differentiation 1, and epidermal growth factor receptor regulate the survival of cochlear sensory epithelial cells.

Journal of neuroscience research

Chen J,Sun W,Zheng Y,Xiong H,Cai Y

Mon Apr 01 00:00:00 EDT 2013

Bone morphogenetic protein 4, inhibitor of differentiation 1, and epidermal growth factor receptor regulate the survival of cochlear sensory epithelial cells.

Journal of neuroscience research

Chen J,Sun W,Zheng Y,Xiong H,Cai Y

Mon Apr 01 00:00:00 EDT 2013

Bone morphogenetic protein 4, inhibitor of differentiation 1, and epidermal growth factor receptor regulate the survival of cochlear sensory epithelial cells.

Journal of neuroscience research

Chen J,Sun W,Zheng Y,Xiong H,Cai Y

Mon Apr 01 00:00:00 EDT 2013

BMP-6 inhibits cell proliferation by targeting microRNA-192 in breast cancer.

Biochimica et biophysica acta

Hu F,Meng X,Tong Q,Liang L,Xiang R,Zhu T,Yang S

Sun Dec 01 00:00:00 EST 2013

BMP-6 inhibits cell proliferation by targeting microRNA-192 in breast cancer.

Biochimica et biophysica acta

Hu F,Meng X,Tong Q,Liang L,Xiang R,Zhu T,Yang S

Sun Dec 01 00:00:00 EST 2013

Exposure to ambient air fine particulate matter prevents VEGF-induced mobilization of endothelial progenitor cells from the bone marrow.

Environmental health perspectives

Haberzettl P,Lee J,Duggineni D,McCracken J,Bolanowski D,O'Toole TE,Bhatnagar A,Conklin DJ

Fri Jun 01 00:00:00 EDT 2012

Exposure to ambient air fine particulate matter prevents VEGF-induced mobilization of endothelial progenitor cells from the bone marrow.

Environmental health perspectives

Haberzettl P,Lee J,Duggineni D,McCracken J,Bolanowski D,O'Toole TE,Bhatnagar A,Conklin DJ

Fri Jun 01 00:00:00 EDT 2012

Exposure to ambient air fine particulate matter prevents VEGF-induced mobilization of endothelial progenitor cells from the bone marrow.

Environmental health perspectives

Haberzettl P,Lee J,Duggineni D,McCracken J,Bolanowski D,O'Toole TE,Bhatnagar A,Conklin DJ

Fri Jun 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: bHLHb24; Class B basic helix-loop-helix protein 24; dJ857M17.1.2; dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein); DNA-binding protein inhibitor ID-1; Inhibitor of differentiation 1; Inhibitor of DNA binding 1; Inhibitor of DNA binding 1 helix-loop-helix protein (splice varaiation); inhibitor of DNA binding 1, dominant negative helix-loop-helix protein; Inhibitor of DNA binding 1, helix-loop-helix protein (splice variation)

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Gene Aliases: AI323524; BHLHB24; D2Wsu140e; ID; Id-1; ID1; ID125A; Idb1

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UniProt ID: (Human) P41134, (Rat) P41135

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Entrez Gene ID: (Human) 3397, (Rat) 25261, (Mouse) 15901

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Function(s)

transcription factor activity, sequence-specific DNA binding protein binding protein C-terminus binding transcription factor binding protein homodimerization activity protein self-association protein N-terminus binding proteasome binding protein dimerization activity DNA-binding transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter angiogenesis endothelial cell morphogenesis transcription, DNA-templated transforming growth factor beta receptor signaling pathway brain development heart development circadian rhythm negative regulation of transcription by transcription factor localization positive regulation of gene expression neuron differentiation BMP signaling pathway protein destabilization positive regulation of actin filament bundle assembly collagen metabolic process cell-abiotic substrate adhesion negative regulation of apoptotic process regulation of MAPK cascade negative regulation of sequence-specific DNA binding transcription factor activity blood vessel endothelial cell migration negative regulation of endothelial cell differentiation negative regulation of osteoblast differentiation regulation of angiogenesis negative regulation of transcription, DNA-templated response to antibiotic blood vessel morphogenesis positive regulation of epithelial cell proliferation negative regulation of dendrite morphogenesis lung morphogenesis lung vasculature development cellular response to epidermal growth factor stimulus negative regulation of protein homodimerization activity cellular response to peptide negative regulation of RNA polymerase II regulatory region sequence-specific DNA binding cellular response to dopamine cellular response to nerve growth factor stimulus response to organic cyclic compound negative regulation of protein binding negative regulation of DNA binding cellular response to transforming growth factor beta stimulus regulation of vasculature development regulation of transcription, DNA-templated multicellular organism development rhythmic process

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ID1 Polyclonal Antibody

ID1 Antibody (18475-1-AP) in ICC/IF

Product Details

18475-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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