Invitrogen

IDH1 Polyclonal Antibody

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This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (23) IDH1 antibodies

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IDH1 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry was performed on human testis tissue using a IDH1 polyclonal antibody (Product # PA5-70042).

IDH1 Antibody in Western Blot (WB) — Western blot was performed using Anti-IDH1 Polyclonal Antibody(Product # PA5-70042) and a 46 kDa band corresponding to IDH1 was observed across cell lines and tissues tested . Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), A-431 (Lane 2), C2C12 (Lane 3), HEK-293 (Lane 4), RAW 264.7 (Lane 5), HeLa (Lane 6), U-2 OS (Lane 7), Mouse Stomach (Lane 8), Mouse Ovary (Lane 9), Mouse Brain (Lane 10), Mouse Pancreas (Lane 11) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

IDH1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Hep G2 cells were transfected with IDH1 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-IDH1 Polyclonal Antibody (Product # PA5-70042). {KD}

Product Details

PA5-70042

Applications	Tested Dilution	Publications
Western Blot (WB)	1.0 µg/mL	-
Immunohistochemistry (IHC)	Assay-dependent	-

Product Specifications
Species Reactivity	Human, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	synthetic peptide directed towards the C terminal region of human IDH1 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 2% sucrose
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2689500

Product Specific Information

This target displays homology in the following species: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%

Target Information

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cytosolic NADP-isocitrate dehydrogenase; epididymis luminal protein 216; epididymis secretory protein Li 26; IDH; IDP; IDPc; isocitrate dehydrogenase 1 (NADP+); isocitrate dehydrogenase 1 (NADP+), soluble; Isocitrate dehydrogenase 1, soluble; Isocitrate dehydrogenase [NADP] cytoplasmic; NADP(+)-specific ICDH; NADP+-specific ICDH; NADP-dependent isocitrate dehydrogenase, cytosolic; NADP-dependent isocitrate dehydrogenase, peroxisomal; OTTHUMP00000206464; OTTHUMP00000206465; OTTHUMP00000206466; Oxalosuccinate decarboxylase

Gene Aliases: HEL-216; HEL-S-26; IDCD; IDH; IDH1; IDP; IDPC; PICD

UniProt ID: (Human) O75874, (Rat) P41562

Entrez Gene ID: (Human) 3417, (Rat) 24479

Molecular Function: dehydrogenase