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NeoBiotechnologies
IDH1-R132G (Isocitrate Dehydrogenase) Recombinant Rabbit Monoclonal Antibody (IDH1.R132G/13054R)
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FIGURE: 1 / 2
IDH1-R132G (Isocitrate Dehydrogenase) Antibody (3417-RBM13-P1) in IHC (P)
Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human prostate cancer stained with IDH1-R132G Recombinant Rabbit Monoclonal Antibody (IDH1. R132G/13054R). Inset: PBS instead of primary antibody; secondary only negative control.
Product Details
3417-RBM13-P1
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
CHO cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
IDH1.R132G/13054R
Immunogen
Recombinant fragment of human IDH1 protein with R132G (exact sequence is proprietary).
Conjugate
Form
Liquid
Concentration
200 µg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS with 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
The IDH1 gene, located on chromosome 2q34, encodes isocitrate dehydrogenase 1, an enzyme that plays a critical role in the citric acid cycle and cellular metabolism by catalyzing the oxidative decarboxylation of isocitrate to alpha-ketoglutarate. The R132G mutation in IDH1 involves the substitution of glycine for arginine at position 132, a hotspot for mutations in various cancers, most notably in gliomas and acute myeloid leukemia (AML). This mutation results in a neomorphic enzyme activity that reduces alpha-ketoglutarate to D-2-hydroxyglutarate, an oncometabolite that contributes to oncogenesis by altering cellular differentiation and epigenetic regulation through inhibition of alpha-ketoglutarate-dependent dioxygenases. The accumulation of D-2-hydroxyglutarate is a hallmark of IDH1-mutant cancers and serves as a diagnostic biomarker. Targeting mutant IDH1 with specific inhibitors has emerged as a therapeutic strategy, aiming to reverse the metabolic and epigenetic aberrations caused by this mutation and provide clinical benefit in cancers harboring this alteration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cytosolic NADP-isocitrate dehydrogenase; epididymis luminal protein 216; epididymis secretory protein Li 26; epididymis secretory sperm binding protein; homodimer; IDH; IDH1; IDH1 R132G; IDPc; isocitrate dehydrogenase (NADP(+)) 1, cytosolic; Isocitrate Dehydrogenase 1 (mutant R132G); isocitrate dehydrogenase 1 (NADP+), soluble; Isocitrate dehydrogenase [NADP] cytoplasmic; NADP(+)-specific ICDH; NADP-dependent isocitrate dehydrogenase, cytosolic; NADP-dependent isocitrate dehydrogenase, peroxisomal; Oxalosuccinate decarboxylase; PICD; soluble; NADP+-dependent; unnamed protein product
Gene Aliases: HEL-216; HEL-S-26; IDCD; IDH; IDH1; IDP; IDPC; PICD
UniProt ID: (Human) O75874
Entrez Gene ID: (Human) 3417
magnesium ion binding
isocitrate dehydrogenase (NADP+) activity
protein binding
oxidoreductase activity
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
identical protein binding
protein homodimerization activity
cadherin binding
metal ion binding
NADP binding
NAD binding
glyoxylate cycle
tricarboxylic acid cycle
isocitrate metabolic process
2-oxoglutarate metabolic process
NADP metabolic process
NADPH regeneration
glutathione metabolic process
response to oxidative stress
female gonad development
response to steroid hormone
regulation of phospholipid catabolic process
regulation of phospholipid biosynthetic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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