Proteintech

IDH2 Polyclonal Antibody

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IDH2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 15932-1-AP (IDH2 antibody) at dilution of 1:400 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

IDH2 Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

IDH2 Antibody in Immunoprecipitation (IP) — IP result of anti-IDH2 (IP:15932-1-AP, 3ug; Detection:15932-1-AP 1:1000) with mouse brain tissue lysate 6000ug.

Product Details

15932-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 12 publications 12 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:200-1:800	-
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	IDH2 Fusion Protein Ag8779 (104-452 aa encoded by BC009244) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.13 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: TQKYSVAVK CATITPDEAR VEEFKLKKMW KSPNGTIRNI LGGTVFREPI ICKNIPRLVP GWTKPITIGR HAHGDQYKAT DFVADRAGTF KMVFTPKDGS GVKEWEVYNF PAGGVGMGMY NTDESISGFA HSCFQYAIQK KWPLYMSTKN TILKAYDGRF KDIFQEIFDK HYKTDFDKNK IWYEHRLIDD MVAQVLKSSG GFVWACKNYD GDVQSDILAQ GFGSLGLMTS VLVCPDGKTI EAEAAHGTVT RHYREHQKGR PTSTNPIASI FAWTRGLEHR GKLDGNQDLI RFAQMLEKVC VETVESGAMT KDLAGCIHGL SNVKLNEHFL NTTDFLDTIK SNLDRALGRQ (104-452 aa encoded by BC009244)

Target Information

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NADas the electron acceptor and the other NADP. Five isocitrate dehydrogenases have been reported: three NAD-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The clinical significance of isocitrate dehydrogenase 2 in esophageal squamous cell carcinoma.

American journal of cancer research

Chen X,Xu W,Wang C,Liu F,Guan S,Sun Y,Wang X,An D,Wen Z,Chen P,Cheng Y

Wed Sep 30 00:00:00 EDT 2020

The clinical significance of isocitrate dehydrogenase 2 in esophageal squamous cell carcinoma.

American journal of cancer research

Chen X,Xu W,Wang C,Liu F,Guan S,Sun Y,Wang X,An D,Wen Z,Chen P,Cheng Y

Wed Sep 30 00:00:00 EDT 2020

Effects of Dahuang zhechong pill on doxorubicin-resistant SMMC-7721 xenografts in mice.

Journal of ethnopharmacology

Wu L,Cao KX,Ni ZH,Li WD,Chen ZP,Cheng HB,Liu X

Fri Aug 10 00:00:00 EDT 2018

Exogenous H₂S switches cardiac energy substrate metabolism by regulating SIRT3 expression in db/db mice.

Journal of molecular medicine (Berlin, Germany)

Sun Y,Tian Z,Liu N,Zhang L,Gao Z,Sun X,Yu M,Wu J,Yang F,Zhao Y,Ren H,Chen H,Zhao D,Wang Y,Dong S,Xu C,Lu F,Zhang W

Sun Apr 01 00:00:00 EDT 2018

Loss of IDH2 Accelerates Age-related Hearing Loss in Male Mice.

Scientific reports

White K,Kim MJ,Han C,Park HJ,Ding D,Boyd K,Walker L,Linser P,Meneses Z,Slade C,Hirst J,Santostefano K,Terada N,Miyakawa T,Tanokura M,Salvi R,Someya S

Thu Mar 22 00:00:00 EDT 2018

Curcumol inhibits the proliferation of gastric adenocarcinoma MGC-803 cells via downregulation of IDH1.

Oncology reports

Zang S,Tang Q,Dong F,Liu H,Li L,Guo F,Pan X,Lin H,Zeng W,Cai Z,Zhong Q,Zang N,Zang L

Fri Dec 01 00:00:00 EST 2017

Loss of NAD-Dependent Protein Deacetylase Sirtuin-2 Alters Mitochondrial Protein Acetylation and Dysregulates Mitophagy.

Antioxidants & redox signaling

Liu G,Park SH,Imbesi M,Nathan WJ,Zou X,Zhu Y,Jiang H,Parisiadou L,Gius D

Sat May 20 00:00:00 EDT 2017

CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.

EMBO reports

Zhong X,Tian S,Zhang X,Diao X,Dong F,Yang J,Li Z,Sun L,Wang L,He X,Wu G,Hu X,Wang L,Song L,Zhang H,Pan X,Li A,Gao P

Mon May 01 00:00:00 EDT 2017

On the Utility of Short Echo Time (TE) Single Voxel 1H-MRS in Non-Invasive Detection of 2-Hydroxyglutarate (2HG); Challenges and Potential Improvement Illustrated with Animal Models Using MRUI and LCModel.

PloS one

Heo H,Kim S,Lee HH,Cho HR,Xu WJ,Lee SH,Park CK,Park S,Choi SH,Kim H

Wed Jul 06 00:00:00 EDT 2016

Redox homeostasis protects mitochondria through accelerating ROS conversion to enhance hypoxia resistance in cancer cells.

Scientific reports

Li P,Zhang D,Shen L,Dong K,Wu M,Ou Z,Shi D

Wed Mar 09 00:00:00 EST 2016

MiR-181a regulates lipid metabolism via IDH1.

Scientific reports

Chu B,Wu T,Miao L,Mei Y,Wu M

Thu Mar 05 00:00:00 EST 2015

Metabolomic comparison between cells over-expressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism.

Journal of neurochemistry

Wen H,Cho HR,Yun T,Kim H,Park CK,Lee SH,Choi SH,Park S

Thu Jan 01 00:00:00 EST 2015

Metabolomic comparison between cells over-expressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism.

Journal of neurochemistry

Wen H,Cho HR,Yun T,Kim H,Park CK,Lee SH,Choi SH,Park S

Thu Jan 01 00:00:00 EST 2015

Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.

Nature communications

Ma X,Li C,Sun L,Huang D,Li T,He X,Wu G,Yang Z,Zhong X,Song L,Gao P,Zhang H

Fri Oct 10 00:00:00 EDT 2014

Short-term cigarette smoke exposure induces reversible changes in energy metabolism and cellular redox status independent of inflammatory responses in mouse lungs.

American journal of physiology. Lung cellular and molecular physiology

Agarwal AR,Zhao L,Sancheti H,Sundar IK,Rahman I,Cadenas E

Thu Nov 15 00:00:00 EST 2012

Bioinformatics

Protein Aliases: ICD-M; IDH; IDP; isocitrate dehydrogenase 2 (NADP+), mitochondrial; Isocitrate dehydrogenase [NADP], mitochondrial; NADP(+)-specific ICDH; NADP+-specific ICDH; NADP+-specific isocitrate dehydrogenase; Oxalosuccinate decarboxylase

Gene Aliases: D2HGA2; E430004F23; ICD-M; IDH; Idh-2; IDH2; IDHM; IDP; IDPM; mNADP-IDH

UniProt ID: (Human) P48735, (Mouse) P54071, (Rat) P56574

Entrez Gene ID: (Human) 3418, (Mouse) 269951, (Rat) 361596

Molecular Function: dehydrogenase