Proteintech

IDH2 Polyclonal Antibody

Product Image Gallery

IDH2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 15932-1-AP (IDH2 antibody) at dilution of 1:400 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

IDH2 Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

IDH2 Antibody in Immunoprecipitation (IP) — IP result of anti-IDH2 (IP:15932-1-AP, 3ug; Detection:15932-1-AP 1:1000) with mouse brain tissue lysate 6000ug.

Product Details

Applications

Tested Dilution

Publications

Immunofluorescence (IF)

Assay-dependent

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:800

Immunoprecipitation (IP)

0.5-4.0 µg

Western Blot (WB)

1:500-1:2000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

IDH2 Fusion Protein Ag8779

Conjugate

Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Target Information

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NADas the electron acceptor and the other NADP. Five isocitrate dehydrogenases have been reported: three NAD-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ICD-M; IDH; IDP; isocitrate dehydrogenase 2 (NADP+), mitochondrial; Isocitrate dehydrogenase [NADP], mitochondrial; NADP(+)-specific ICDH; NADP+-specific ICDH; NADP+-specific isocitrate dehydrogenase; Oxalosuccinate decarboxylase

Gene Aliases: D2HGA2; E430004F23; ICD-M; IDH; Idh-2; IDH2; IDHM; IDP; IDPM; mNADP-IDH

UniProt ID: (Human) P48735, (Mouse) P54071, (Rat) P56574

Entrez Gene ID: (Human) 3418, (Mouse) 269951, (Rat) 361596

Function(s)

carbohydrate metabolic process glyoxylate cycle tricarboxylic acid cycle isocitrate metabolic process 2-oxoglutarate metabolic process oxidation-reduction process

Process(es)

magnesium ion binding isocitrate dehydrogenase (NADP+) activity NAD binding oxidoreductase activity oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor metal ion binding