Proteintech

IDH2 Polyclonal Antibody

IDH2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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IDH2 Antibody (15932-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 15932-1-AP (IDH2 antibody) at dilution of 1:400 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

IDH2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

15932-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 33 publications 33 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:800

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

IDH2 Fusion Protein Ag8779 (104-452 aa encoded by BC009244)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: TQKYSVAVK CATITPDEAR VEEFKLKKMW KSPNGTIRNI LGGTVFREPI ICKNIPRLVP GWTKPITIGR HAHGDQYKAT DFVADRAGTF KMVFTPKDGS GVKEWEVYNF PAGGVGMGMY NTDESISGFA HSCFQYAIQK KWPLYMSTKN TILKAYDGRF KDIFQEIFDK HYKTDFDKNK IWYEHRLIDD MVAQVLKSSG GFVWACKNYD GDVQSDILAQ GFGSLGLMTS VLVCPDGKTI EAEAAHGTVT RHYREHQKGR PTSTNPIASI FAWTRGLEHR GKLDGNQDLI RFAQMLEKVC VETVESGAMT KDLAGCIHGL SNVKLNEHFL NTTDFLDTIK SNLDRALGRQ (104-452 aa encoded by BC009244)

Target Information

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NADas the electron acceptor and the other NADP. Five isocitrate dehydrogenases have been reported: three NAD-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Astaxanthin promotes mitochondrial biogenesis and antioxidant capacity in chronic high-intensity interval training.

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Non-oxidative pentose phosphate pathway controls regulatory T cell function by integrating metabolism and epigenetics.

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Fan TW,Daneshmandi S,Cassel TA,Uddin MB,Sledziona J,Thompson PT,Lin P,Higashi RM,Lane AN

Tue Nov 01 00:00:00 UTC 2022

Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.

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Zhang S,Huang W,Ren L,Ju X,Gong M,Rao J,Sun L,Li P,Ding Q,Wang J,Zhang QC

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Sun May 01 00:00:00 UTC 2022

MUC1 triggers lineage plasticity of Her2 positive mammary tumors.

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Sun May 01 00:00:00 UTC 2022

MUC1 triggers lineage plasticity of Her2 positive mammary tumors.

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Pang Z,Dong X,Deng H,Wang C,Liao X,Liao C,Liao Y,Tian W,Cheng J,Chen G,Yi H,Huang L

Sun May 01 00:00:00 UTC 2022

MUC1 triggers lineage plasticity of Her2 positive mammary tumors.

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Sun May 01 00:00:00 UTC 2022

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Tue Aug 30 00:00:00 UTC 2022

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Sodium cantharidate induces Apoptosis in breast cancer cells by regulating energy metabolism via the protein phosphatase 5-p53 axis.

Toxicology and applied pharmacology

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Loss of the mitochondrial phosphate carrier SLC25A3 induces remodeling of the cardiac mitochondrial protein acylome.

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Wed Sep 01 00:00:00 UTC 2021

Loss of the mitochondrial phosphate carrier SLC25A3 induces remodeling of the cardiac mitochondrial protein acylome.

American journal of physiology. Cell physiology

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Wed Sep 01 00:00:00 UTC 2021

Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.

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STAT3-dependent analysis reveals PDK4 as independent predictor of recurrence in prostate cancer.

Molecular systems biology

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Isocitrate dehydrogenase 2 contributes to radiation resistance of oesophageal squamous cell carcinoma via regulating mitochondrial function and ROS/pAKT signalling.

British journal of cancer

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Isocitrate dehydrogenase 2 contributes to radiation resistance of oesophageal squamous cell carcinoma via regulating mitochondrial function and ROS/pAKT signalling.

British journal of cancer

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Two human patient mitochondrial pyruvate carrier mutations reveal distinct molecular mechanisms of dysfunction.

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Journal of molecular medicine (Berlin, Germany)

Sun Y,Tian Z,Liu N,Zhang L,Gao Z,Sun X,Yu M,Wu J,Yang F,Zhao Y,Ren H,Chen H,Zhao D,Wang Y,Dong S,Xu C,Lu F,Zhang W

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Exogenous H₂S switches cardiac energy substrate metabolism by regulating SIRT3 expression in db/db mice.

Journal of molecular medicine (Berlin, Germany)

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Wu L,Cao KX,Ni ZH,Li WD,Chen ZP,Cheng HB,Liu X

Fri Aug 10 00:00:00 UTC 2018

Effects of Dahuang zhechong pill on doxorubicin-resistant SMMC-7721 xenografts in mice.

Journal of ethnopharmacology

Wu L,Cao KX,Ni ZH,Li WD,Chen ZP,Cheng HB,Liu X

Fri Aug 10 00:00:00 UTC 2018

Loss of IDH2 Accelerates Age-related Hearing Loss in Male Mice.

Scientific reports

White K,Kim MJ,Han C,Park HJ,Ding D,Boyd K,Walker L,Linser P,Meneses Z,Slade C,Hirst J,Santostefano K,Terada N,Miyakawa T,Tanokura M,Salvi R,Someya S

Thu Mar 22 00:00:00 UTC 2018

Loss of IDH2 Accelerates Age-related Hearing Loss in Male Mice.

Scientific reports

White K,Kim MJ,Han C,Park HJ,Ding D,Boyd K,Walker L,Linser P,Meneses Z,Slade C,Hirst J,Santostefano K,Terada N,Miyakawa T,Tanokura M,Salvi R,Someya S

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Zhong X,Tian S,Zhang X,Diao X,Dong F,Yang J,Li Z,Sun L,Wang L,He X,Wu G,Hu X,Wang L,Song L,Zhang H,Pan X,Li A,Gao P

Mon May 01 00:00:00 UTC 2017

CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.

EMBO reports

Zhong X,Tian S,Zhang X,Diao X,Dong F,Yang J,Li Z,Sun L,Wang L,He X,Wu G,Hu X,Wang L,Song L,Zhang H,Pan X,Li A,Gao P

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Antioxidants & redox signaling

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Loss of NAD-Dependent Protein Deacetylase Sirtuin-2 Alters Mitochondrial Protein Acetylation and Dysregulates Mitophagy.

Antioxidants & redox signaling

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Sat May 20 00:00:00 UTC 2017

The clinical significance of isocitrate dehydrogenase 2 in esophageal squamous cell carcinoma.

American journal of cancer research

Chen X,Xu W,Wang C,Liu F,Guan S,Sun Y,Wang X,An D,Wen Z,Chen P,Cheng Y

Wed Sep 30 00:00:00 UTC 2020

The clinical significance of isocitrate dehydrogenase 2 in esophageal squamous cell carcinoma.

American journal of cancer research

Chen X,Xu W,Wang C,Liu F,Guan S,Sun Y,Wang X,An D,Wen Z,Chen P,Cheng Y

Wed Sep 30 00:00:00 UTC 2020

The clinical significance of isocitrate dehydrogenase 2 in esophageal squamous cell carcinoma.

American journal of cancer research

Chen X,Xu W,Wang C,Liu F,Guan S,Sun Y,Wang X,An D,Wen Z,Chen P,Cheng Y

Wed Sep 30 00:00:00 UTC 2020

The clinical significance of isocitrate dehydrogenase 2 in esophageal squamous cell carcinoma.

American journal of cancer research

Chen X,Xu W,Wang C,Liu F,Guan S,Sun Y,Wang X,An D,Wen Z,Chen P,Cheng Y

Wed Sep 30 00:00:00 UTC 2020

Curcumol inhibits the proliferation of gastric adenocarcinoma MGC-803 cells via downregulation of IDH1.

Oncology reports

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Fri Dec 01 00:00:00 UTC 2017

Curcumol inhibits the proliferation of gastric adenocarcinoma MGC-803 cells via downregulation of IDH1.

Oncology reports

Zang S,Tang Q,Dong F,Liu H,Li L,Guo F,Pan X,Lin H,Zeng W,Cai Z,Zhong Q,Zang N,Zang L

Fri Dec 01 00:00:00 UTC 2017

SIRT3-Mediated Dimerization of IDH2 Directs Cancer Cell Metabolism and Tumor Growth.

Cancer research

Zou X,Zhu Y,Park SH,Liu G,O'Brien J,Jiang H,Gius D

Tue Aug 01 00:00:00 UTC 2017

Redox homeostasis protects mitochondria through accelerating ROS conversion to enhance hypoxia resistance in cancer cells.

Scientific reports

Li P,Zhang D,Shen L,Dong K,Wu M,Ou Z,Shi D

Wed Mar 09 00:00:00 UTC 2016

Redox homeostasis protects mitochondria through accelerating ROS conversion to enhance hypoxia resistance in cancer cells.

Scientific reports

Li P,Zhang D,Shen L,Dong K,Wu M,Ou Z,Shi D

Wed Mar 09 00:00:00 UTC 2016

On the Utility of Short Echo Time (TE) Single Voxel 1H-MRS in Non-Invasive Detection of 2-Hydroxyglutarate (2HG); Challenges and Potential Improvement Illustrated with Animal Models Using MRUI and LCModel.

PloS one

Heo H,Kim S,Lee HH,Cho HR,Xu WJ,Lee SH,Park CK,Park S,Choi SH,Kim H

Wed Jul 06 00:00:00 UTC 2016

PloS one

Heo H,Kim S,Lee HH,Cho HR,Xu WJ,Lee SH,Park CK,Park S,Choi SH,Kim H

Wed Jul 06 00:00:00 UTC 2016

Metabolomic comparison between cells over-expressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism.

Journal of neurochemistry

Wen H,Cho HR,Yun T,Kim H,Park CK,Lee SH,Choi SH,Park S

Thu Jan 01 00:00:00 UTC 2015

Metabolomic comparison between cells over-expressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism.

Journal of neurochemistry

Wen H,Cho HR,Yun T,Kim H,Park CK,Lee SH,Choi SH,Park S

Thu Jan 01 00:00:00 UTC 2015

Metabolomic comparison between cells over-expressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism.

Journal of neurochemistry

Wen H,Cho HR,Yun T,Kim H,Park CK,Lee SH,Choi SH,Park S

Thu Jan 01 00:00:00 UTC 2015

Metabolomic comparison between cells over-expressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism.

Journal of neurochemistry

Wen H,Cho HR,Yun T,Kim H,Park CK,Lee SH,Choi SH,Park S

Thu Jan 01 00:00:00 UTC 2015

MiR-181a regulates lipid metabolism via IDH1.

Scientific reports

Chu B,Wu T,Miao L,Mei Y,Wu M

Thu Mar 05 00:00:00 UTC 2015

MiR-181a regulates lipid metabolism via IDH1.

Scientific reports

Chu B,Wu T,Miao L,Mei Y,Wu M

Thu Mar 05 00:00:00 UTC 2015

Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.

Nature communications

Ma X,Li C,Sun L,Huang D,Li T,He X,Wu G,Yang Z,Zhong X,Song L,Gao P,Zhang H

Fri Oct 10 00:00:00 UTC 2014

Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.

Nature communications

Ma X,Li C,Sun L,Huang D,Li T,He X,Wu G,Yang Z,Zhong X,Song L,Gao P,Zhang H

Fri Oct 10 00:00:00 UTC 2014

Short-term cigarette smoke exposure induces reversible changes in energy metabolism and cellular redox status independent of inflammatory responses in mouse lungs.

American journal of physiology. Lung cellular and molecular physiology

Agarwal AR,Zhao L,Sancheti H,Sundar IK,Rahman I,Cadenas E

Thu Nov 15 00:00:00 UTC 2012

Short-term cigarette smoke exposure induces reversible changes in energy metabolism and cellular redox status independent of inflammatory responses in mouse lungs.

American journal of physiology. Lung cellular and molecular physiology

Agarwal AR,Zhao L,Sancheti H,Sundar IK,Rahman I,Cadenas E

Thu Nov 15 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: ICD-M; IDH; IDP; isocitrate dehydrogenase 2 (NADP+), mitochondrial; Isocitrate dehydrogenase [NADP], mitochondrial; NADP(+)-specific ICDH; NADP+-specific ICDH; NADP+-specific isocitrate dehydrogenase; Oxalosuccinate decarboxylase

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Gene Aliases: D2HGA2; E430004F23; ICD-M; IDH; Idh-2; IDH2; IDHM; IDP; IDPM; mNADP-IDH

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UniProt ID: (Human) P48735, (Mouse) Q9EQK1, (Rat) P56574

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Entrez Gene ID: (Human) 3418, (Mouse) 269951, (Rat) 361596

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Function(s)

magnesium ion binding isocitrate dehydrogenase (NADP+) activity NAD binding oxidoreductase activity oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor metal ion binding dehydrogenase

Process(es)

carbohydrate metabolic process glyoxylate cycle tricarboxylic acid cycle isocitrate metabolic process 2-oxoglutarate metabolic process oxidation-reduction process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IDH2 Polyclonal Antibody

IDH2 Antibody (15932-1-AP) in IHC (P)

Product Details

15932-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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