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Invitrogen
IDH2 Polyclonal Antibody
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FIGURE: 1 / 6
IDH2 Antibody (PA5-79436) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of IDH2 in A549 cells using IDH2 Polyclonal Antibody (Product # PA5-79436). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. DyLight 488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets ... View More
Product Details
PA5-79436
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human IDH2 (413-447aa KDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDR).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Jurkat whole cell, human THP-1 whole cell, human MCF-7 whole cell, human HEL whole cell, rat heart tissue, rat liver tissue, mouse heart tissue, mouse liver tissue. IHC: human colon cancer tissue, human ovarican cancer tissue, mouse heart tissue, rat heart tissue. ICC/IF: A549 cell .
Target Information
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NADas the electron acceptor and the other NADP. Five isocitrate dehydrogenases have been reported: three NAD-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ICD-M; IDH; IDP; isocitrate dehydrogenase (NADP(+)) 2, mitochondrial; isocitrate dehydrogenase 2 (NADP+), mitochondrial; Isocitrate dehydrogenase [NADP], mitochondrial; NADP(+)-specific ICDH; NADP+-specific ICDH; NADP+-specific isocitrate dehydrogenase; Oxalosuccinate decarboxylase; unnamed protein product
Gene Aliases: D2HGA2; E430004F23; ICD-M; IDH; IDH-2; IDH2; IDHM; IDP; IDPM; mNADP-IDH
UniProt ID: (Human) P48735, (Mouse) P54071, (Rat) P56574
Entrez Gene ID: (Human) 3418, (Mouse) 269951, (Rat) 361596
carbohydrate metabolic process
glyoxylate cycle
tricarboxylic acid cycle
isocitrate metabolic process
2-oxoglutarate metabolic process
NADP metabolic process
NADP biosynthetic process
negative regulation of glial cell proliferation
negative regulation of glial cell migration
negative regulation of matrix metallopeptidase secretion
oxidation-reduction process
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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