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Bethyl Laboratories
IFIT3 Polyclonal Antibody
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FIGURE: 1 / 1
IFIT3 Antibody (A305-196A) in IP
Detection of human IFIT3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IFIT3 antibody (Product # A305-196A) (lot 1) used for IP at 6 µg per reaction. IFIT3 was also immunoprecipitated by rabbit anti-IFIT3 antibody A305-197A. For blotting immunoprecipitated IFIT3, A305-196A was used at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
Product Details
A305-196A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 325 to 375 of human Interferon-induced protein with tetratricopeptide repeats 3.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Chimpanzee
Target Information
IFN-induced antiviral protein which acts as an inhibitor of cellular as well as viral processes, cell migration, proliferation, signaling, and viral replication. Enhances MAVS-mediated host antiviral responses by serving as an adapter bridging TBK1 to MAVS which leads to the activation of TBK1 and phosphorylation of IRF3 and phosphorylated IRF3 translocates into nucleus to promote antiviral gene transcription. Exhibits an antiproliferative activity via the up-regulation of cell cycle negative regulators CDKN1A/p21 and CDKN1B/p27. Normally, CDKN1B/p27 turnover is regulated by COPS5, which binds CDKN1B/p27 in the nucleus and exports it to the cytoplasm for ubiquitin-dependent degradation. IFIT3 sequesters COPS5 in the cytoplasm, thereby increasing nuclear CDKN1B/p27 protein levels. Upregulates CDKN1A/p21 by downregulating MYC, a repressor of CDKN1A/p21. Can negatively regulate the apoptotic effects of IFIT2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CIG49; IFI-60K; IFIT-3; IFIT-4; Interferon-induced 60 kDa protein; Interferon-induced protein with tetratricopeptide repeats 3; Interferon-induced protein with tetratricopeptide repeats 4; ISG-60; P60; Retinoic acid-induced gene G protein; RP11-149I23.4
Gene Aliases: CIG-49; cig41; GARG-49; IFI60; IFIT3; IFIT4; IRG2; ISG60; P60; RIG-G
UniProt ID: (Human) O14879
Entrez Gene ID: (Human) 3437
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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