Proteintech

IFITM3 Polyclonal Antibody

Product Image Gallery

IFITM3 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 11714-1-AP (IFITM3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

IFITM3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 11714-1-AP (IFITM3 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

IFITM3 Antibody in Western Blot (WB) — Mouse colon tissue were subjected to SDS PAGE followed by western blot with 11714-1-AP ( IFITM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IFITM3 Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 0.2ug IFITM3 antibody (11714-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

IFITM3 Antibody in Immunoprecipitation (IP) — IP result of anti-IFITM3 (IP:11714-1-AP, 3ug; Detection:11714-1-AP 1:1500) with HeLa cells lysate 2400ug.

Product Details

11714-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 37 publications 37 publications
Immunohistochemistry (IHC)	-	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:500	View 9 publications 9 publications
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	View 4 publications 4 publications
Immunoprecipitation (IP)	0.5-4.0 µg	View 2 publications 2 publications
Miscellaneous PubMed (Misc)	-	View 4 publications 4 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Goat, Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	IFITM3 Fusion Protein Ag2285 (1-133 aa encoded by BC006794) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.39 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

11714-1-AP is a rabbit polyclonal antibody raised against the full-length of human IFITM3.

Immunogen sequence: MNHTVQTFF SPVNSGQPPN YEMLKEEHEV AVLGAPHNPA PPTSTVIHIR SETSVPDHVV WSLFNTLFMN PCCLGFIAFA YSVKSRDRKM VGDVTGAQAY ASTAKCLNIW ALILGILMTI LLIVIPVLIF QAYG (1-133 aa encoded by BC006794)

Target Information

IFITM3 (interferon induced transmembrane protein 3), also known as 1-8U or IP15, is a multi-pass membrane protein that belongs to the IFITM (interferon inducible transmembrane) family of proteins. IFITM proteins are induced by type I and type II interferons and contain multiple interferon (IFN)-stimulated response elements (ISREs) in their promoter regions. IFITM proteins play important roles in many cellular processes and their expression requires the presence of the chromatin remodeling SWI/SNF-like BAF complexes. Cellular processes involving IFITM proteins include cellular anti-proliferative activities and homotypic cell adhesion functions of interferons. In addition, IFITM genes are often upregulated in various cancer cells, suggesting a possible role in carcinogenesis. Localizing to the membrane, IFITM3 is a 133 amino acid protein that is induced by IFN-alpha and IFN-gamma. IFITM3 expression can be regulated by TEF-1, Brg-1 and Sp1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Host Restriction Factor Interferon-Inducible Transmembrane Protein 3 Inhibits Vaccinia Virus Infection.

Frontiers in immunology

Li C,Du S,Tian M,Wang Y,Bai J,Tan P,Liu W,Yin R,Wang M,Jiang Y,Li Y,Zhu N,Zhu Y,Li T,Wu S,Jin N,He F

Fri Mar 29 00:00:00 EDT 2019

IFITM3 directly engages and shuttles incoming virus particles to lysosomes.

Nature chemical biology

Spence JS,He R,Hoffmann HH,Das T,Thinon E,Rice CM,Peng T,Chandran K,Hang HC

Fri Mar 01 00:00:00 EST 2019

IFITM3 directly engages and shuttles incoming virus particles to lysosomes.

Nature chemical biology

Spence JS,He R,Hoffmann HH,Das T,Thinon E,Rice CM,Peng T,Chandran K,Hang HC

Fri Mar 01 00:00:00 EST 2019

Functional Mapping of Regions Involved in the Negative Imprinting of Virion Particle Infectivity and in Target Cell Protection by Interferon-Induced Transmembrane Protein 3 against HIV-1.

Journal of virology

Appourchaux R,Delpeuch M,Zhong L,Burlaud-Gaillard J,Tartour K,Savidis G,Brass A,Etienne L,Roingeard P,Cimarelli A

Tue Jan 15 00:00:00 EST 2019

Functional Mapping of Regions Involved in the Negative Imprinting of Virion Particle Infectivity and in Target Cell Protection by Interferon-Induced Transmembrane Protein 3 against HIV-1.

Journal of virology

Appourchaux R,Delpeuch M,Zhong L,Burlaud-Gaillard J,Tartour K,Savidis G,Brass A,Etienne L,Roingeard P,Cimarelli A

Tue Jan 15 00:00:00 EST 2019

The β₄ subunit of Ca_v1.2 channels is required for an optimal interferon response in cardiac muscle cells.

Science signaling

Tammineni ER,Carrillo ED,Soto-Acosta R,Angel-Ambrocio AH,García MC,Bautista-Carbajal P,Del Angel RM,Sánchez JA

Tue Dec 11 00:00:00 EST 2018

Cyclosporine H Overcomes Innate Immune Restrictions to Improve Lentiviral Transduction and Gene Editing In Human Hematopoietic Stem Cells.

Cell stem cell

Petrillo C,Thorne LG,Unali G,Schiroli G,Giordano AMS,Piras F,Cuccovillo I,Petit SJ,Ahsan F,Noursadeghi M,Clare S,Genovese P,Gentner B,Naldini L,Towers GJ,Kajaste-Rudnitski A

Thu Dec 06 00:00:00 EST 2018

Cyclosporine H Overcomes Innate Immune Restrictions to Improve Lentiviral Transduction and Gene Editing In Human Hematopoietic Stem Cells.

Cell stem cell

Petrillo C,Thorne LG,Unali G,Schiroli G,Giordano AMS,Piras F,Cuccovillo I,Petit SJ,Ahsan F,Noursadeghi M,Clare S,Genovese P,Gentner B,Naldini L,Towers GJ,Kajaste-Rudnitski A

Thu Dec 06 00:00:00 EST 2018

IFITM3 Restricts Human Metapneumovirus Infection.

The Journal of infectious diseases

McMichael TM,Zhang Y,Kenney AD,Zhang L,Zani A,Lu M,Chemudupati M,Li J,Yount JS

Fri Oct 05 00:00:00 EDT 2018

Role of MxB in Alpha Interferon-Mediated Inhibition of HIV-1 Infection.

Journal of virology

Xu B,Pan Q,Liang C

Sat Sep 01 00:00:00 EDT 2018

The Inhibition of HIV-1 Entry Imposed by Interferon Inducible Transmembrane Proteins Is Independent of Co-Receptor Usage.

Viruses

Yu J,Liu SL

Tue Aug 07 00:00:00 EDT 2018

The Inhibition of HIV-1 Entry Imposed by Interferon Inducible Transmembrane Proteins Is Independent of Co-Receptor Usage.

Viruses

Yu J,Liu SL

Tue Aug 07 00:00:00 EDT 2018

Late Endosomal/Lysosomal Cholesterol Accumulation Is a Host Cell-Protective Mechanism Inhibiting Endosomal Escape of Influenza A Virus.

mBio

Kühnl A,Musiol A,Heitzig N,Johnson DE,Ehrhardt C,Grewal T,Gerke V,Ludwig S,Rescher U

Tue Jul 24 00:00:00 EDT 2018

Selective Enrichment and Direct Analysis of Protein S-Palmitoylation Sites.

Journal of proteome research

Thinon E,Fernandez JP,Molina H,Hang HC

Fri May 04 00:00:00 EDT 2018

Argininosuccinate synthetase 1 contributes to gastric cancer invasion and progression by modulating autophagy.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Tsai CY,Chi HC,Chi LM,Yang HY,Tsai MM,Lee KF,Huang HW,Chou LF,Cheng AJ,Yang CW,Wang CS,Lin KH

Tue May 01 00:00:00 EDT 2018

A CRISPR screen for factors regulating SAMHD1 degradation identifies IFITMs as potent inhibitors of lentiviral particle delivery.

Retrovirology

Roesch F,OhAinle M,Emerman M

Tue Mar 20 00:00:00 EDT 2018

The palmitoyltransferase ZDHHC20 enhances interferon-induced transmembrane protein 3 (IFITM3) palmitoylation and antiviral activity.

The Journal of biological chemistry

McMichael TM,Zhang L,Chemudupati M,Hach JC,Kenney AD,Hang HC,Yount JS

Fri Dec 29 00:00:00 EST 2017

The palmitoyltransferase ZDHHC20 enhances interferon-induced transmembrane protein 3 (IFITM3) palmitoylation and antiviral activity.

The Journal of biological chemistry

McMichael TM,Zhang L,Chemudupati M,Hach JC,Kenney AD,Hang HC,Yount JS

Fri Dec 29 00:00:00 EST 2017

Reactive Oxygen Species Regulate Both Priming and Established Arthritis, but with Different Mechanisms.

Antioxidants & redox signaling

Sareila O,Hagert C,Kelkka T,Linja M,Xu B,Kihlberg J,Holmdahl R

Wed Dec 20 00:00:00 EST 2017

Histone demethylase LSD1 restricts influenza A virus infection by erasing IFITM3-K88 monomethylation.

PLoS pathogens

Shan J,Zhao B,Shan Z,Nie J,Deng R,Xiong R,Tsun A,Pan W,Zhao H,Chen L,Jin Y,Qian Z,Lui K,Liang R,Li D,Sun B,Lavillette D,Xu K,Li B

Fri Dec 01 00:00:00 EST 2017

IFITM3 requires an amphipathic helix for antiviral activity.

EMBO reports

Chesarino NM,Compton AA,McMichael TM,Kenney AD,Zhang L,Soewarna V,Davis M,Schwartz O,Yount JS

Sun Oct 01 00:00:00 EDT 2017

Nonhuman Primate IFITM Proteins Are Potent Inhibitors of HIV and SIV.

PloS one

Wilkins J,Zheng YM,Yu J,Liang C,Liu SL

Wed Jul 26 00:00:00 EDT 2017

Zika virus induces massive cytoplasmic vacuolization and paraptosis-like death in infected cells.

The EMBO journal

Monel B,Compton AA,Bruel T,Amraoui S,Burlaud-Gaillard J,Roy N,Guivel-Benhassine F,Porrot F,Génin P,Meertens L,Sinigaglia L,Jouvenet N,Weil R,Casartelli N,Demangel C,Simon-Lorière E,Moris A,Roingeard P,Amara A,Schwartz O

Wed Jun 14 00:00:00 EDT 2017

Zika virus induces massive cytoplasmic vacuolization and paraptosis-like death in infected cells.

The EMBO journal

Wed Jun 14 00:00:00 EDT 2017

Antiviral Role of IFITM Proteins in African Swine Fever Virus Infection.

PloS one

Muñoz-Moreno R,Cuesta-Geijo MÁ,Martínez-Romero C,Barrado-Gil L,Galindo I,García-Sastre A,Alonso C

Mon Mar 27 00:00:00 EDT 2017

Antiviral Role of IFITM Proteins in African Swine Fever Virus Infection.

PloS one

Muñoz-Moreno R,Cuesta-Geijo MÁ,Martínez-Romero C,Barrado-Gil L,Galindo I,García-Sastre A,Alonso C

Mon Mar 27 00:00:00 EDT 2017

Virion Background and Efficiency of Virion Incorporation Determine Susceptibility of Simian Immunodeficiency Virus Env-Driven Viral Entry to Inhibition by IFITM Proteins.

Journal of virology

Wrensch F,Hoffmann M,Gärtner S,Nehlmeier I,Winkler M,Pöhlmann S

Sun Jan 15 00:00:00 EST 2017

The Interferon-Stimulated Gene IFITM3 Restricts Infection and Pathogenesis of Arthritogenic and Encephalitic Alphaviruses.

Journal of virology

Poddar S,Hyde JL,Gorman MJ,Farzan M,Diamond MS

Sat Oct 01 00:00:00 EDT 2016

The Interferon-Stimulated Gene Ifitm3 Restricts West Nile Virus Infection and Pathogenesis.

Journal of virology

Gorman MJ,Poddar S,Farzan M,Diamond MS

Thu Sep 15 00:00:00 EDT 2016

Infection of goats with goatpox virus triggers host antiviral defense through activation of innate immune signaling.

Research in veterinary science

Zeng X,Wang S,Chi X,Chen SL,Huang S,Lin Q,Xie B,Chen JL

Mon Feb 01 00:00:00 EST 2016

IFITM Proteins Restrict HIV-1 Infection by Antagonizing the Envelope Glycoprotein.

Cell reports

Yu J,Li M,Wilkins J,Ding S,Swartz TH,Esposito AM,Zheng YM,Freed EO,Liang C,Chen BK,Liu SL

Tue Oct 06 00:00:00 EDT 2015

IFITM Proteins Restrict HIV-1 Infection by Antagonizing the Envelope Glycoprotein.

Cell reports

Yu J,Li M,Wilkins J,Ding S,Swartz TH,Esposito AM,Zheng YM,Freed EO,Liang C,Chen BK,Liu SL

Tue Oct 06 00:00:00 EDT 2015

Wif1 and Ifitm3 gene expression preferentially altered in the colon mucosa of benzo[a]pyrene pre-treated mice following exposure to dextran sulfate sodium.

Chemico-biological interactions

Koyama N,Hakura A,Toritsuka N,Sonoda J,Seki Y,Tohyama O,Asakura S,Nakano-Ito K,Hosokawa S

Mon Oct 05 00:00:00 EDT 2015

Expression profile and histological distribution of IFITM1 and IFITM3 during H9N2 avian influenza virus infection in BALB/c mice.

Medical microbiology and immunology

Yu M,Qi W,Huang Z,Zhang K,Ye J,Liu R,Wang H,Ma Y,Liao M,Ning Z

Sat Aug 01 00:00:00 EDT 2015

E3 Ubiquitin Ligase NEDD4 Promotes Influenza Virus Infection by Decreasing Levels of the Antiviral Protein IFITM3.

PLoS pathogens

Chesarino NM,McMichael TM,Yount JS

Sat Aug 01 00:00:00 EDT 2015

Muscovy duck reovirus infection rapidly activates host innate immune signaling and induces an effective antiviral immune response involving critical interferons.

Veterinary microbiology

Chen Z,Luo G,Wang Q,Wang S,Chi X,Huang Y,Wei H,Wu B,Huang S,Chen JL

Wed Feb 25 00:00:00 EST 2015

Human respiratory syncytial virus infection is inhibited by IFN-induced transmembrane proteins.

The Journal of general virology

Zhang W,Zhang L,Zan Y,Du N,Yang Y,Tien P

Thu Jan 01 00:00:00 EST 2015

Human respiratory syncytial virus infection is inhibited by IFN-induced transmembrane proteins.

The Journal of general virology

Zhang W,Zhang L,Zan Y,Du N,Yang Y,Tien P

Thu Jan 01 00:00:00 EST 2015

IFITM3-containing exosome as a novel mediator for anti-viral response in dengue virus infection.

Cellular microbiology

Zhu X,He Z,Yuan J,Wen W,Huang X,Hu Y,Lin C,Pan J,Li R,Deng H,Liao S,Zhou R,Wu J,Li J,Li M

Thu Jan 01 00:00:00 EST 2015

Primate lentiviruses are differentially inhibited by interferon-induced transmembrane proteins.

Virology

Qian J,Le Duff Y,Wang Y,Pan Q,Ding S,Zheng YM,Liu SL,Liang C

Thu Jan 01 00:00:00 EST 2015

IFITM proteins incorporated into HIV-1 virions impair viral fusion and spread.

Cell host & microbe

Compton AA,Bruel T,Porrot F,Mallet A,Sachse M,Euvrard M,Liang C,Casartelli N,Schwartz O

Wed Dec 10 00:00:00 EST 2014

IFITM proteins incorporated into HIV-1 virions impair viral fusion and spread.

Cell host & microbe

Compton AA,Bruel T,Porrot F,Mallet A,Sachse M,Euvrard M,Liang C,Casartelli N,Schwartz O

Wed Dec 10 00:00:00 EST 2014

IFITM proteins are incorporated onto HIV-1 virion particles and negatively imprint their infectivity.

Retrovirology

Tartour K,Appourchaux R,Gaillard J,Nguyen XN,Durand S,Turpin J,Beaumont E,Roch E,Berger G,Mahieux R,Brand D,Roingeard P,Cimarelli A

Tue Nov 25 00:00:00 EST 2014

T cell memory. Skin-resident memory CD8⁺ T cells trigger a state of tissue-wide pathogen alert.

Science (New York, N.Y.)

Ariotti S,Hogenbirk MA,Dijkgraaf FE,Visser LL,Hoekstra ME,Song JY,Jacobs H,Haanen JB,Schumacher TN

Fri Oct 03 00:00:00 EDT 2014

Influenza A virus-induced degradation of eukaryotic translation initiation factor 4B contributes to viral replication by suppressing IFITM3 protein expression.

Journal of virology

Wang S,Chi X,Wei H,Chen Y,Chen Z,Huang S,Chen JL

Fri Aug 01 00:00:00 EDT 2014

Identification of an endocytic signal essential for the antiviral action of IFITM3.

Cellular microbiology

Jia R,Xu F,Qian J,Yao Y,Miao C,Zheng YM,Liu SL,Guo F,Geng Y,Qiao W,Liang C

Tue Jul 01 00:00:00 EDT 2014

Interferon induction of IFITM proteins promotes infection by human coronavirus OC43.

Proceedings of the National Academy of Sciences of the United States of America

Zhao X,Guo F,Liu F,Cuconati A,Chang J,Block TM,Guo JT

Tue May 06 00:00:00 EDT 2014

Negative regulation of interferon-induced transmembrane protein 3 by SET7-mediated lysine monomethylation.

The Journal of biological chemistry

Shan Z,Han Q,Nie J,Cao X,Chen Z,Yin S,Gao Y,Lin F,Zhou X,Xu K,Fan H,Qian Z,Sun B,Zhong J,Li B,Tsun A

Fri Dec 06 00:00:00 EST 2013

IFITM-2 and IFITM-3 but not IFITM-1 restrict Rift Valley fever virus.

Journal of virology

Mudhasani R,Tran JP,Retterer C,Radoshitzky SR,Kota KP,Altamura LA,Smith JM,Packard BZ,Kuhn JH,Costantino J,Garrison AR,Schmaljohn CS,Huang IC,Farzan M,Bavari S

Thu Aug 01 00:00:00 EDT 2013

Knockdown of interferon-induced transmembrane protein 3 expression suppresses breast cancer cell growth and colony formation and affects the cell cycle.

Oncology reports

Yang M,Gao H,Chen P,Jia J,Wu S

Mon Jul 01 00:00:00 EDT 2013

Identification of host proteins involved in Japanese encephalitis virus infection by quantitative proteomics analysis.

Journal of proteome research

Zhang LK,Chai F,Li HY,Xiao G,Guo L

Fri Jun 07 00:00:00 EDT 2013

TMPRSS2 activates the human coronavirus 229E for cathepsin-independent host cell entry and is expressed in viral target cells in the respiratory epithelium.

Journal of virology

Bertram S,Dijkman R,Habjan M,Heurich A,Gierer S,Glowacka I,Welsch K,Winkler M,Schneider H,Hofmann-Winkler H,Thiel V,Pöhlmann S

Sat Jun 01 00:00:00 EDT 2013

IFITM proteins restrict viral membrane hemifusion.

PLoS pathogens

Li K,Markosyan RM,Zheng YM,Golfetto O,Bungart B,Li M,Ding S,He Y,Liang C,Lee JC,Gratton E,Cohen FS,Liu SL

Tue Jan 01 00:00:00 EST 2013

The N-terminal region of IFITM3 modulates its antiviral activity by regulating IFITM3 cellular localization.

Journal of virology

Jia R,Pan Q,Ding S,Rong L,Liu SL,Geng Y,Qiao W,Liang C

Sat Dec 01 00:00:00 EST 2012

The N-terminal region of IFITM3 modulates its antiviral activity by regulating IFITM3 cellular localization.

Journal of virology

Jia R,Pan Q,Ding S,Rong L,Liu SL,Geng Y,Qiao W,Liang C

Sat Dec 01 00:00:00 EST 2012

IFITM3 restricts the morbidity and mortality associated with influenza.

Nature

Everitt AR,Clare S,Pertel T,John SP,Wash RS,Smith SE,Chin CR,Feeley EM,Sims JS,Adams DJ,Wise HM,Kane L,Goulding D,Digard P,Anttila V,Baillie JK,Walsh TS,Hume DA,Palotie A,Xue Y,Colonna V,Tyler-Smith C,Dunning J,Gordon SB,Smyth RL,Openshaw PJ,Dougan G,Brass AL,Kellam P

Sun Mar 25 00:00:00 EDT 2012

The IFITM proteins inhibit HIV-1 infection.

Journal of virology

Lu J,Pan Q,Rong L,He W,Liu SL,Liang C

Tue Mar 01 00:00:00 EST 2011

Interferon-induced cell membrane proteins, IFITM3 and tetherin, inhibit vesicular stomatitis virus infection via distinct mechanisms.

Journal of virology

Weidner JM,Jiang D,Pan XB,Chang J,Block TM,Guo JT

Wed Dec 01 00:00:00 EST 2010

Interferon-induced cell membrane proteins, IFITM3 and tetherin, inhibit vesicular stomatitis virus infection via distinct mechanisms.

Journal of virology

Weidner JM,Jiang D,Pan XB,Chang J,Block TM,Guo JT

Wed Dec 01 00:00:00 EST 2010

Identification of five interferon-induced cellular proteins that inhibit west nile virus and dengue virus infections.

Journal of virology

Jiang D,Weidner JM,Qing M,Pan XB,Guo H,Xu C,Zhang X,Birk A,Chang J,Shi PY,Block TM,Guo JT

Sun Aug 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: Dispanin subfamily A member 2b; DSPA2b; Fragilis; Fragilis 3; Fragilis protein; ifitm-like protein 1; Interferon-induced transmembrane protein 3; Interferon-inducible protein 1-8U; Interferon-inducible protein 15; interferon-inducible protein variant 10; Mil-1; Mouse ifitm-like protein 1

Gene Aliases: 1-8U; 1110004C05Rik; Cd225; Cdw217; DSPA2b; Fgls; IFITM3; IP15; mil-1; rat8

UniProt ID: (Human) Q01628, (Rat) P26376, (Mouse) Q9CQW9

Entrez Gene ID: (Human) 10410, (Rat) 361673, (Mouse) 66141

Function(s)