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Invitrogen
IFN gamma Monoclonal Antibody (R4-6A2), Functional Grade, eBioscience™
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FIGURE: 1 / 2
IFN gamma Antibody (16-7312-81) in Flow
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2024 -
2 FIGURE DMI regulated Th17 responses, but not Th1 or Treg responses, in vivo. Flow cytometry for (A) IL-17 (Th17), (B) IFN-gamma (Th1), and (C) Foxp3 (Treg) expression in CD4 + T cell from spleens of immunized mice. (D) The levels of IL-17, IFN-gamma, TGF-beta1, and IL-10 protein in sera of immunized mice at day 24 after EAU induction were determined via ELISA. (E) The relative expressions of Rorc, Il17a, ... View More
Product Details
16-7312-81
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
C. elegans,
Human,
Mouse
Host/Isotype
Rat
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
R4-6A2
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The R4-6A2 antibody reacts with mouse interferon-gamma (IFN-gamma ), a homodimeric 15-20 kDa cytokine secreted by Th1 cells. The R4-6A2 antibody is a neutralizing antibody. IFN-gamma is an ~20 kDa factor produced by activated T, B and NK cells and is an anti-viral and anti-parasitic cytokine. IFN-gamma in synergy with other cytokines, such as TNF-alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN-gamma. Glycosylation of IFN-gamma does not affect its biological activity.
Applications Reported:The R4-6A2 antibody has been reported for use in cytokine neutralization.
Applications Tested: The Functional Grade Purified R4-6A2 antibody has been tested by LAL assay to verify low endotoxin levels and has been tested in bioassay for neutralization of IFN gamma bioactivity.
The R4-6A2 antibody at 15 ng/mL has been found to inhibit by 50% the biological effects of 1 ng/mL mouse IFN gamma (ND50), in an EMCV assay of L929 cell protection. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level:Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation:Less than 10%, as determined by HPLC.
Target Information
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: gamma interferon; H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Interferon; Interferon gamma; Interferon y; Interferon γ; Interferonγ; M-IFN-g; R-IFN-g
Gene Aliases: Ifg; IFN-g; Ifng
UniProt ID: (Mouse) P01580
Entrez Gene ID: (Mouse) 15978
protein import into nucleus, translocation
negative regulation of transcription from RNA polymerase II promoter
neutrophil apoptotic process
regulation of the force of heart contraction
adaptive immune response
CD8-positive, alpha-beta T cell differentiation involved in immune response
regulation of transcription, DNA-templated
apoptotic process
inflammatory cell apoptotic process
immune response
humoral immune response
cell cycle arrest
positive regulation of cell proliferation
negative regulation of cell proliferation
response to virus
positive regulation of epithelial cell migration
antigen processing and presentation
neutrophil chemotaxis
negative regulation of epithelial cell differentiation
endoplasmic reticulum unfolded protein response
negative regulation of myelination
positive regulation of synaptic transmission, cholinergic
negative regulation of interleukin-17 production
positive regulation of interleukin-12 production
positive regulation of interleukin-23 production
positive regulation of tumor necrosis factor production
positive regulation of peptidyl-serine phosphorylation of STAT protein
positive regulation of smooth muscle cell apoptotic process
regulation of growth
positive regulation of T cell proliferation
positive regulation of tyrosine phosphorylation of Stat1 protein
defense response to bacterium
defense response to protozoan
negative regulation of growth of symbiont in host
negative regulation of growth of symbiont involved in interaction with host
positive regulation of chemokine biosynthetic process
positive regulation of interleukin-12 biosynthetic process
positive regulation of MHC class II biosynthetic process
positive regulation of interleukin-6 biosynthetic process
positive regulation of nitric oxide biosynthetic process
positive regulation of neuron differentiation
positive regulation of osteoclast differentiation
positive regulation of cell adhesion
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of isotype switching to IgG isotypes
negative regulation of smooth muscle cell proliferation
positive regulation of interleukin-1 beta secretion
regulation of immune response
regulation of insulin secretion
sensory perception of mechanical stimulus
positive regulation of membrane protein ectodomain proteolysis
defense response to virus
positive regulation of killing of cells of other organism
positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity
positive regulation of fructose 1,6-bisphosphate metabolic process
positive regulation of vitamin D biosynthetic process
positive regulation of calcidiol 1-monooxygenase activity
cellular response to lipopolysaccharide
cellular response to interleukin-18
extrinsic apoptotic signaling pathway
regulation of neuronal action potential
positive regulation of tumor necrosis factor (ligand) superfamily member 11 production
regulation of hepatocyte proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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