Immunofluorescent analysis of IFT80 showing staining in the centrosome of NT2D1 cells. NT2D1 cells were fixed in ice-cold MeOH for 5 min and stained using an IFT80 polyclonal antibody (Product # PA5-28970) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 114 and 362 of Human IFT80|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-28970 targets IFT80 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-28970 immunogen is recombinant fragment corresponding to a region within amino acids 114 and 362 of Human IFT80.
The IFT80 gene encodes a protein with 7 WD40 domains that is a component of the intraflagellar transport (IFT) complex B (Beales et al., 2007 [PubMed 17468754]). The IFT is essential for the development and maintenance of motile and sensory cilia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.