Invitrogen

IFT88 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2 Published Figures

4 References

View all (9) IFT88 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

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IFT88 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of IFT88 in HepG2 cells using a IFT88 monoclonal antibody (Product # PA5-18467) at 10 µg/mL for1hr. The cells were paraformaldehyde fixed and permeabilized with 0.15% Triton. Primary incubation was followed by Alexa Fluor 488 secondary antibody (2 µg/mL) showing nuclear and Golgi/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL)followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

IFT88 Antibody in Western Blot (WB) — Western blot was performed using Anti-IFT88 Polyclonal Antibody (Product # PA5-18467) and band at 120 kDa corresponding to IFT88 was observed across the cell lines and tissues tested. Whole cell extracts (30ug lysate) of Hep G2 (Lane 1), COLO 205 (Lane 2), SK-OV-3 (Lane 3), NIH/3T3 (Lane 4), Jurkat (Lane 5), HEK-293 (Lane 6), MDCK (Lane 7), tissue extracts (30 µg lysate) of Mouse Testis (Lane 8) and Rat Testis (Lane 9) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/ml) and detected by Rabbit anti-Goat IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Uncharacterized bands at ~70 and ~90 kDa were observed in some of the samples tested.

IFT88 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of IFT88 in HepG2 cells using a polyclonal antibody (Product #PA5-18467). HepG2 cells (blue line) were paraformaldehyde fixed and permeabilized with 0.5% Triton. The primary antibody was incubated for one hour (10 µg/mL) followed by an Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by an Alexa Fluor 488 secondary antibody.

IFT88 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Hep G2 cells were transfected with IFT88 siRNA and loss of signal was observed in Western Blot using IFT88 Polyclonal Antibody (Product # PA5-18467). {KD}

Product Details

PA5-18467

Applications	Tested Dilution	Publications
Western Blot (WB)	1-3 µg/mL	View 2 publications 2 publications
Immunocytochemistry (ICC/IF)	10 µg/mL	View 1 publication 1 publication
Flow Cytometry (Flow)	10 µg/mL	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse
Published species	Dog, Human, Mouse
Host/Isotype	Goat / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide sequence (KKRIDEDDFADEE) corresponding to the C-terminus amino acids of IFT88
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	TBS, pH 7.3, with 0.5% BSA
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_10979641

Product Specific Information

This antibody is predicted to react with canine and rat based on sequence homology.

Target Information

This gene encodes a member of the tetratrico peptide repeat family. Mutations of a similar gene in mouse can cause polycystic kidney disease. Two transcript variants encoding distinct isoforms have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cilia proteins are biomarkers of altered flow in the vasculature.

JCI insight

Gupta A,Thirugnanam K,Thamilarasan M,Mohieldin AM,Zedan HT,Prabhudesai S,Griffin MR,Spearman AD,Pan A,Palecek SP,Yalcin HC,Nauli SM,Rarick KR,Zennadi R,Ramchandran R

PA5-18467 was used in Western Blot to suggest that cilia and ciliary proteins in circulation are detectable under various altered-flow conditions, which could serve as a surrogate biomarker of the damaged endothelium.

Tue Mar 22 00:00:00 EDT 2022

Cilia proteins are biomarkers of altered flow in the vasculature.

JCI insight

Gupta A,Thirugnanam K,Thamilarasan M,Mohieldin AM,Zedan HT,Prabhudesai S,Griffin MR,Spearman AD,Pan A,Palecek SP,Yalcin HC,Nauli SM,Rarick KR,Zennadi R,Ramchandran R

Tue Mar 22 00:00:00 EDT 2022

Ciliogenesis-coupled accumulation of IFT-B proteins in a novel cytoplasmic compartment.

Genes to cells : devoted to molecular & cellular mechanisms

Lamri L,Twan WK,Katoh TA,Botilde Y,Takaoka K,Ikawa Y,Nishimura H,Fukumoto A,Minegishi K,Mizuno K,Hamada H

PA5-18467 was used in Immunocytochemistry to examine the behaviors of the Cluap1 protein, a ciliary protein that belongs to the IFT-B complex and is required for ciliogenesis, in nonciliated and ciliated cells.

Fri Nov 01 00:00:00 EDT 2019

Dicam promotes proliferation and maturation of chondrocyte through Indian hedgehog signaling in primary cilia.

Osteoarthritis and cartilage

Han S,Park HR,Lee EJ,Jang JA,Han MS,Kim GW,Jeong JH,Choi JY,Beier F,Jung YK

PA5-18467 was used in Immunohistochemistry-immunofluorescence to show that, in chondrocytes, Dicam can modulate primary cilia-mediated Indian hedgehog signaling to promote elongation of long bone.

Sun Jul 01 00:00:00 EDT 2018

Ciliary membrane proteins traffic through the Golgi via a Rabep1/GGA1/Arl3-dependent mechanism.

Nature communications

Kim H,Xu H,Yao Q,Li W,Huang Q,Outeda P,Cebotaru V,Chiaravalli M,Boletta A,Piontek K,Germino GG,Weinman EJ,Watnick T,Qian F

PA5-18467 was used in western blot to elucidate how polycystin-1 and -2 traffic to cilia

Tue Nov 18 00:00:00 EST 2014

Bioinformatics

Protein Aliases: HGNC:20606; intraflagellar transport 88 homolog; Intraflagellar transport protein 88 homolog; MGC26259; polaris homolog; probe hTg737 (polycystic kidney disease, autosomal recessive); Recessive polycystic kidney disease protein Tg737; Recessive polycystic kidney disease protein Tg737 homolog; RP11-172H24.2; testicular tissue protein Li 93; tetratricopeptide repeat domain 10; Tetratricopeptide repeat protein 10; Tg737 protein; TgN(Imorpk)737Rpw; TPR repeat protein 10; transgene insert site 737, insertional mutation, polycystic kidney disease

Gene Aliases: AW552028; D13S1056E; DAF19; flexo; fxo; hTg737; IFT88; orpk; polaris; TG737; Tg737Rpw; TgN737Rpw; TTC10

UniProt ID: (Human) Q13099, (Mouse) Q61371

Entrez Gene ID: (Human) 8100, (Mouse) 21821

Function(s)

cilium morphogenesis regulation of cilium assembly regulation of autophagosome assembly eye development kidney development liver development epithelial cell morphogenesis Notch signaling pathway smoothened signaling pathway sperm axoneme assembly spermatid nucleus elongation determination of left/right symmetry nervous system development brain development heart development protein localization epidermis development regulation of smoothened signaling pathway organ morphogenesis anterior/posterior pattern specification dorsal/ventral pattern formation spinal cord dorsal/ventral patterning telencephalon development cell projection organization lung development pancreas development cytoplasmic microtubule organization response to fluid shear stress nonmotile primary cilium assembly epidermal stem cell homeostasis cilium assembly regulation of odontogenesis of dentin-containing tooth embryonic digit morphogenesis positive regulation of insulin-like growth factor receptor signaling pathway regulation of fat cell differentiation forebrain morphogenesis negative regulation of epithelial cell proliferation cardiac muscle cell differentiation palate development inner ear receptor stereocilium organization limb development regulation of feeding behavior cardiac septum morphogenesis lung vasculature development heart formation neural precursor cell proliferation regulation of protein processing cochlea development

Process(es)

protein binding kinesin binding

IFT88 Polyclonal Antibody

Product Details

PA5-18467

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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