|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant human IGF-I.|
|Storage buffer||PBS, pH 7.2|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.5-2.0 ug/ml|
|Functional Assay (FN)||0.67-1.0 µg/ml|
|Western Blot (WB)||0.1-0.2ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Reconstitute with 0.1 ml of distilled water. For long term storage the addition of 0.09% sodium azide is recommended (unless the product will be used for functional studies). Prior to reconstitution, store at 4°C. After reconstitution, store undiluted at -20°C, avoiding freeze/thaw cycles.
This antibody may be used in indirect ELISA or as a capture reagent in sandwich ELISA with PA1-86912 as a detection reagent.
IGF-1 (Insulin-like Growth Factor I) is a 70 amino acid secreted mitogenic polypeptide and member of the insulin gene family, produced primarily by the liver. IGF-I exhibits both autocrine and paracrine properties and has a widespread effect on cells including those of the bone cartilage, muscle, nerves, skin, liver and lungs, playing a key role in cell proliferation and growth, inhibition of apoptosis and linked with tumour cell growth. IGF-I signals through binding to the type 1 insulin-like growth factor receptor (IGF-1R) and also to the insulin receptor (IR), resulting in the activation of the AKT signalling pathway. Production of IGF-I is stimulated by growth hormone (GH) and can be hindered by GH insensitivity, deficiency in GH receptors or a malfunction in the post GH receptor signalling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.