Proteintech

IGF1B Polyclonal Antibody

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Questions & Answers

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IGF1B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 20215-1-AP (IGF1B-Specific antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

IGF1B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 20215-1-AP (IGF1B-Specific antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

IGF1B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 20215-1-AP (IGF1B-Specific antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

IGF1B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 20215-1-AP (IGF1B-Specific antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

IGF1B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse liver tissue slide using 20215-1-AP (IGF1B-Specific antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

IGF1B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse liver tissue slide using 20215-1-AP (IGF1B-Specific antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

IGF1B Antibody in Western Blot (WB) — Human brain tissue were subjected to SDS PAGE followed by western blot with 20215-1-AP (IGF1B-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Product Details

Applications

Tested Dilution

Publications

Immunofluorescence (IF)

Assay-dependent

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

-

Western Blot (WB)

Assay-dependent

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

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Product Specifications

Species Reactivity

Human

Published species

Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated

Form

Liquid

Concentration

0.44 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

The antibody is specific to isoform IGF-1B.

Target Information

Insulin-like growth factor-1 (IGF1), is structurally and functionally related to insulin but has a much higher growth-promoting activity. A variety of cellular responses are induced by IGF1, including cell proliferation, differentiation, migration, and survival. IGF1 stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regards to enhancing glucose uptake. In circulation, IGFs are predominantly bound to binding proteins (IGFBPs) which prolong the half-life of the IGFs and play a role in delivering them to target tissues. Low levels of IGF1 have been linked to Alzheimer's disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: IGF-I; insulin-like growth factor 1 (somatomedin C); Insulin-like growth factor I; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin-C

Gene Aliases: IBP1; IGF-I; IGF1; IGFI; MGF

UniProt ID: (Human) P05019

Entrez Gene ID: (Human) 3479

activation of MAPK activity skeletal system development response to hypoxia cell activation platelet degranulation DNA replication regulation of translation movement of cell or subcellular component signal transduction Ras protein signal transduction muscle organ development female pregnancy aging response to nutrient memory cell proliferation positive regulation of cell proliferation response to heat glycolate metabolic process regulation of gene expression positive regulation of glycoprotein biosynthetic process positive regulation of cardiac muscle hypertrophy phosphatidylinositol 3-kinase signaling positive regulation of phosphatidylinositol 3-kinase signaling response to activity skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration muscle hypertrophy myotube cell development positive regulation of smooth muscle cell migration proteoglycan biosynthetic process positive regulation of cell migration response to caffeine activation of protein kinase B activity response to lipopolysaccharide cellular response to insulin stimulus positive regulation of protein import into nucleus, translocation negative regulation of smooth muscle cell apoptotic process response to nicotine bone mineralization involved in bone maturation regulation of multicellular organism growth positive regulation of activated T cell proliferation positive regulation of tyrosine phosphorylation of Stat5 protein negative regulation of apoptotic process positive regulation of DNA binding positive regulation of MAPK cascade protein kinase B signaling positive regulation of insulin-like growth factor receptor signaling pathway cellular protein metabolic process myoblast differentiation positive regulation of fat cell differentiation positive regulation of osteoblast differentiation positive regulation of glycogen biosynthetic process positive regulation of DNA replication positive regulation of glycolytic process positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import positive regulation of Ras protein signal transduction insulin-like growth factor receptor signaling pathway phosphatidylinositol-mediated signaling positive regulation of fibroblast proliferation positive regulation of smooth muscle cell proliferation positive regulation of epithelial cell proliferation positive regulation of protein secretion positive regulation of peptidyl-tyrosine phosphorylation protein stabilization detection of mechanical stimulus involved in sensory perception response to glucocorticoid myoblast proliferation regulation of calcium ion transport negative regulation of oocyte development positive regulation of cell growth involved in cardiac muscle cell development ERK1 and ERK2 cascade positive regulation of calcineurin-NFAT signaling cascade cellular response to electrical stimulus cellular response to estradiol stimulus negative regulation of release of cytochrome c from mitochondria negative regulation of neuron death positive regulation of trophectodermal cell proliferation negative regulation of cholangiocyte apoptotic process positive regulation of transcription regulatory region DNA binding negative regulation of extrinsic apoptotic signaling pathway

Process(es)

insulin receptor binding insulin-like growth factor receptor binding integrin binding hormone activity steroid binding protein binding growth factor activity