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Invitrogen
IGF1R (CD221) Polyclonal Antibody
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FIGURE: 1 / 4
IGF1R (CD221) Antibody (PA5-79444) in ICC/IF
Immunofluorescence analysis of Insulin-like growth factor 1 receptor was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with IGF1R (CD221) Polyclonal Antibody (Product # PA5-79444) at 5 µg/mL in 0.1% BSA, i... View More
Product Details
PA5-79444
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human IGF1 Receptor (1353-1367aa RKNERALPLPQSSTC).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
4mg trehalose, 0.9mg NaCl, 0.2mg Na2PO4
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: 293T Cell, A549 Cell, MCF-7 Cell. IHC: Rat Pancreas Tissue.
Target Information
IGF1R, also known as Insulin-like Growth Factor 1 (IGF-1) Receptor, is a receptor tyrosine kinase activated by IGF-1 and IGF-2. IGFR has been implicated in a variety of cancers. The protein is a tetramer consisting of two alpha, and two beta subunits. The alpha-subunits binds to insulin growth factor 1 with higher affinity than to insulin growth factor 2. Binding results in a conformational change, followed by autophosphorylation of IGF-1R at positions 1134, 1135, 1136. The activated IGF-1R in turn, causes phosphorylation of substrate proteins, followed by sequential activation of RAS, RAF, and mitogen-activated protein kinase isoforms ERK, p38, and JNK, leading to the transcription of genes that drive proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD221; CD221 antigen; IGF 1 receptor; IGF 1R; IGF-I receptor; Insulin like growth factor 1 receptor precursor; Insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; line 186
Gene Aliases: A330103N21Rik; CD221; D930020L01; hyft; IGF-1R; IGF1R; IGFIR; IGFIRC; IGFR; Igfr1; JTK13
UniProt ID: (Human) P08069, (Rat) P24062, (Mouse) Q60751
Entrez Gene ID: (Human) 3480, (Rat) 25718, (Mouse) 16001
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
insulin-activated receptor activity
insulin-like growth factor-activated receptor activity
insulin receptor binding
structural molecule activity
protein binding
insulin-like growth factor binding
ATP binding
kinase activity
transferase activity
insulin-like growth factor I binding
identical protein binding
phosphatidylinositol 3-kinase binding
insulin binding
insulin receptor substrate binding
protein transporter activity
G-protein alpha-subunit binding
transferase activity, transferring phosphorus-containing groups
transmembrane signal receptor
immune response
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
positive regulation of cell proliferation
insulin receptor signaling pathway
positive regulation of cell migration
peptidyl-tyrosine autophosphorylation
negative regulation of apoptotic process
positive regulation of protein complex disassembly
negative regulation of MAPK cascade
positive regulation of MAPK cascade
protein kinase B signaling
transcytosis
regulation of JNK cascade
protein autophosphorylation
insulin-like growth factor receptor signaling pathway
positive regulation of protein kinase B signaling
cellular response to glucose stimulus
dendritic spine maintenance
beta-amyloid clearance
positive regulation of cold-induced thermogenesis
cellular response to oxygen-containing compound
cellular response to beta-amyloid
protein phosphorylation
axonogenesis
brain development
epidermis development
organ morphogenesis
negative regulation of muscle cell apoptotic process
establishment of cell polarity
male sex determination
mammary gland development
exocrine pancreas development
neuron projection development
positive regulation of cytokinesis
response to vitamin E
negative regulation of sequence-specific DNA binding transcription factor activity
positive regulation of DNA replication
positive regulation of mitotic nuclear division
phosphatidylinositol-mediated signaling
positive regulation of DNA metabolic process
protein tetramerization
protein heterooligomerization
inactivation of MAPKK activity
negative regulation of protein kinase B signaling
prostate gland epithelium morphogenesis
positive regulation of steroid hormone biosynthetic process
phosphorylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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