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Invitrogen
IGF1R (CD221) Polyclonal Antibody
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FIGURE: 1 / 3
IGF1R (CD221) Antibody (PA5-79445) in IHC (P)
Immunohistochemistry analysis of IGF1R (CD221) on paraffin-embedded mouse small intestine tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with IGF1R (CD221) polyclonal antibody (Product# PA5-79445) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 ... View More
Product Details
PA5-79445
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived mouse IGF1 Receptor recombinant protein (Position: E31-K257).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: mouse liver tissue. IHC: mouse small intestine tissue, mouse kidney tissue.
Target Information
IGF1R, also known as Insulin-like Growth Factor 1 (IGF-1) Receptor, is a receptor tyrosine kinase activated by IGF-1 and IGF-2. IGFR has been implicated in a variety of cancers. The protein is a tetramer consisting of two alpha, and two beta subunits. The alpha-subunits binds to insulin growth factor 1 with higher affinity than to insulin growth factor 2. Binding results in a conformational change, followed by autophosphorylation of IGF-1R at positions 1134, 1135, 1136. The activated IGF-1R in turn, causes phosphorylation of substrate proteins, followed by sequential activation of RAS, RAF, and mitogen-activated protein kinase isoforms ERK, p38, and JNK, leading to the transcription of genes that drive proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD221; CD221 antigen; IGF 1 receptor; IGF 1R; IGF-I receptor; Insulin like growth factor 1 receptor precursor; Insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; line 186
Gene Aliases: A330103N21Rik; CD221; D930020L01; hyft; IGF-1R; Igf1r; IGFIRC; Igfr1; JTK13
UniProt ID: (Rat) P24062, (Mouse) Q60751
Entrez Gene ID: (Rat) 25718, (Mouse) 16001
G-protein alpha-subunit binding
protein tyrosine kinase activity
insulin-like growth factor-activated receptor activity
insulin receptor binding
insulin-like growth factor binding
ATP binding
insulin-like growth factor I binding
identical protein binding
phosphatidylinositol 3-kinase binding
insulin binding
insulin receptor substrate binding
nucleotide binding
protein kinase activity
transmembrane receptor protein tyrosine kinase activity
protein binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
transmembrane signal receptor
protein phosphorylation
immune response
axonogenesis
brain development
epidermis development
organ morphogenesis
negative regulation of muscle cell apoptotic process
establishment of cell polarity
male sex determination
positive regulation of cell migration
mammary gland development
exocrine pancreas development
neuron projection development
positive regulation of cytokinesis
response to vitamin E
peptidyl-tyrosine autophosphorylation
negative regulation of apoptotic process
negative regulation of MAPK cascade
positive regulation of MAPK cascade
negative regulation of sequence-specific DNA binding transcription factor activity
positive regulation of DNA replication
positive regulation of mitotic nuclear division
regulation of JNK cascade
protein autophosphorylation
insulin-like growth factor receptor signaling pathway
phosphatidylinositol-mediated signaling
positive regulation of DNA metabolic process
protein tetramerization
protein heterooligomerization
inactivation of MAPKK activity
positive regulation of protein kinase B signaling
negative regulation of protein kinase B signaling
prostate gland epithelium morphogenesis
positive regulation of steroid hormone biosynthetic process
transmembrane receptor protein tyrosine kinase signaling pathway
phosphorylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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