Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 3
IGF2 Antibody (500-P12-100UG) in WB
Western Blot: To detect Human IGF-II by Western Blot analysis IGF2 Polyclonal Antibody (Product # 500-P12-1MG) can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IGF-II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P12-100UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human IGF-II
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929321
Product Specific Information
AA Sequence of recombinant protein: AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IGF-II. Anti-Human IGF-II specific antibody was purified by affinity chromatography employing an immobilized Human IGF-II matrix.
Sandwich ELISA: To detect Human IGF-II by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IGF-II (500-P12Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IGF-II.
Western Blot: To detect Human IGF-II by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IGF-II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
The IGFs are mitogenic, polypeptide growth factors that stimulate the proliferation and survival of various cell types, including muscle, bone, and cartilage tissue in vitro. IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar to insulin by structure and function, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: FLJ22066; FLJ44734; H-IGF-2; IGF; IGF-II; Insulin like growth factor; insulin-like growth factor 2 (somatomedin A); Insulin-like growth factor II; insulin-like growth factor type 2; preptin; Somatomedin-A; T3M-11-derived growth factor
Gene Aliases: C11orf43; GRDF; IGF-II; IGF2; PP1446; PP9974
UniProt ID: (Human) P01344
Entrez Gene ID: (Human) 3481
skeletal system development
ossification
positive regulation of protein phosphorylation
platelet degranulation
glucose metabolic process
regulation of gene expression by genetic imprinting
regulation of transcription, DNA-templated
multicellular organism development
female pregnancy
memory
positive regulation of cell proliferation
insulin receptor signaling pathway
response to radiation
response to nutrient levels
response to estradiol
response to nicotine
insulin receptor signaling pathway via phosphatidylinositol 3-kinase
wound healing
positive regulation of activated T cell proliferation
response to drug
positive regulation of catalytic activity
positive regulation of MAPK cascade
cellular protein metabolic process
response to ethanol
positive regulation of glycogen biosynthetic process
positive regulation of mitotic nuclear division
negative regulation of natural killer cell mediated cytotoxicity
positive regulation of insulin receptor signaling pathway
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of cell division
positive regulation of protein kinase B signaling
cellular response to mechanical stimulus
positive regulation of protein serine/threonine kinase activity
positive regulation of receptor activity
positive regulation of glycogen (starch) synthase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support