Proteintech

IGF2BP2 Polyclonal Antibody

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IGF2BP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using (Product # 11601-1-AP) (IGF2BP2 antibody) at dilution of 1:1,000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IGF2BP2 Antibody in Western Blot (WB) — Human kidney tissue were subjected to SDS PAGE followed by western blot with 11601-1-AP (IGF2BP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IGF2BP2 Antibody in Immunoprecipitation (IP) — IP result of anti-IGF2BP2 (IP:11601-1-AP, 3ug; Detection:11601-1-AP 1:500) with HEK-293 cells lysate 1000ug.

Product Details

11601-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 56 publications 56 publications

Immunohistochemistry (IHC)

View 18 publications 18 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 7 publications 7 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 8 publications 8 publications

RNA Immunoprecipitation (RIP)

View 29 publications 29 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

IGF2BP2 Fusion Protein Ag2142 (1-341 aa encoded by BC021290)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

This antibody raise against part of N-terminus of human IGF2BP2.

Immunogen sequence: MNKLYIGNL SPAVTADDLR QLFGDRKLPL AGQVLLKSGY AFVDYPDQNW AIRAIETLSG KVELHGKIME VDYSVSKKLR SRKIQIRNIP PHLQWEVLDG LLAQYGTVEN VEQVNTDTET AVVNVTYATR EEAKIAMEKL SGHQFENYSF KISYIPDEEV SSPSPPQRAQ RGDHSSREQG HAPGGTSQAR QIDFPLRILV PTQFVGAIIG KEGLTIKNIT KQTQSRVDIH RKENSGAAEK PVTIHATPEG TSEACRMILE IMQKEADETK LAEEIPLKIL AHNGLVGRLI GKEGRNLKKI EHETGTKITI SSLQDLSIYN PERTITVKGT VEACASAEIE IM (1-341 aa encoded by BC021290)

Target Information

IGF2BP2 is a member of the IGF-II mRNA-binding protein (IMP) family. It recruits target transcripts to cytoplasmic protein-RNA complexes. The protein contains several four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Autoantibodies against IGF2BP2 are detected in sera from some patients with hepatocellular carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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m⁶A Modification of Long Non-Coding RNA HNF1A-AS1 Facilitates Cell Cycle Progression in Colorectal Cancer via IGF2BP2-Mediated CCND1 mRNA Stabilization.

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METTL14-mediated epitranscriptome modification of MN1 mRNA promote tumorigenicity and all-trans-retinoic acid resistance in osteosarcoma.

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Mon Aug 01 00:00:00 EDT 2022

CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m⁶A-modified CREB1 mRNA.

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N⁶-methyladenosine regulates maternal RNA maintenance in oocytes and timely RNA decay during mouse maternal-to-zygotic transition.

Nature cell biology

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Wed Jun 01 00:00:00 EDT 2022

m6A methylated EphA2 and VEGFA through IGF2BP2/3 regulation promotes vasculogenic mimicry in colorectal cancer via PI3K/AKT and ERK1/2 signaling.

Cell death & disease

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Sat May 21 00:00:00 EDT 2022

CircEIF3H-IGF2BP2-HuR scaffold complex promotes TNBC progression via stabilizing HSPD1/RBM8A/G3BP1 mRNA.

Cell death discovery

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Sat May 14 00:00:00 EDT 2022

Tumor Suppressor microRNA-138 Suppresses Low-Grade Glioma Development and Metastasis via Regulating IGF2BP2.

OncoTargets and therapy

Yang Y,Liu X,Cheng L,Li L,Wei Z,Wang Z,Han G,Wan X,Wang Z,Zhang J,Chen C

Wed Apr 13 00:00:00 EDT 2022

METTL3-mediated m⁶A modification of TIMP2 mRNA promotes podocyte injury in diabetic nephropathy.

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Wed Apr 06 00:00:00 EDT 2022

Four m6A RNA Methylation Gene Signatures and Their Prognostic Values in Lung Adenocarcinoma.

Technology in cancer research & treatment

Wang Y,Zhao X,Li J,Wang X,Hu W,Zhang X

Tue Apr 05 00:00:00 EDT 2022

AS3MT facilitates NLRP3 inflammasome activation by m⁶A modification during arsenic-induced hepatic insulin resistance.

Cell biology and toxicology

Qiu T,Wu C,Yao X,Han Q,Wang N,Yuan W,Zhang J,Shi Y,Jiang L,Liu X,Yang G,Sun X

Mon Feb 28 00:00:00 EST 2022

IGF2BP2 Induces U251 Glioblastoma Cell Chemoresistance by Inhibiting FOXO1-Mediated PID1 Expression Through Stabilizing lncRNA DANCR.

Frontiers in cell and developmental biology

Han J,Yu X,Wang S,Wang Y,Liu Q,Xu H,Wang X

Wed Feb 16 00:00:00 EST 2022

Methyltransferase-like 14 suppresses growth and metastasis of renal cell carcinoma by decreasing long noncoding RNA NEAT1.

Cancer science

Liu T,Wang H,Fu Z,Wang Z,Wang J,Gan X,Wang A,Wang L

Tue Feb 01 00:00:00 EST 2022

FTO suppresses glycolysis and growth of papillary thyroid cancer via decreasing stability of APOE mRNA in an N6-methyladenosine-dependent manner.

Journal of experimental & clinical cancer research : CR

Huang J,Sun W,Wang Z,Lv C,Zhang T,Zhang D,Dong W,Shao L,He L,Ji X,Zhang P,Zhang H

Fri Jan 28 00:00:00 EST 2022

Mettl3 promotes oxLDL-mediated inflammation through activating STAT1 signaling.

Journal of clinical laboratory analysis

Li Z,Xu Q,Huangfu N,Chen X,Zhu J

Sat Jan 01 00:00:00 EST 2022

MNSFβ regulates placental development by conjugating IGF2BP2 to enhance trophoblast cell invasiveness.

Cell proliferation

Yang Q,Ma Y,Liu Y,Shao X,Jia W,Yu X,Li YX,Yang L,Gu W,Wang H,Wang J,Wang YL

Wed Dec 01 00:00:00 EST 2021

MNSFβ regulates placental development by conjugating IGF2BP2 to enhance trophoblast cell invasiveness.

Cell proliferation

Yang Q,Ma Y,Liu Y,Shao X,Jia W,Yu X,Li YX,Yang L,Gu W,Wang H,Wang J,Wang YL

Wed Dec 01 00:00:00 EST 2021

MNSFβ regulates placental development by conjugating IGF2BP2 to enhance trophoblast cell invasiveness.

Cell proliferation

Yang Q,Ma Y,Liu Y,Shao X,Jia W,Yu X,Li YX,Yang L,Gu W,Wang H,Wang J,Wang YL

Wed Dec 01 00:00:00 EST 2021

An in vitro system to silence mitochondrial gene expression.

Cell

Cruz-Zaragoza LD,Dennerlein S,Linden A,Yousefi R,Lavdovskaia E,Aich A,Falk RR,Gomkale R,Schöndorf T,Bohnsack MT,Richter-Dennerlein R,Urlaub H,Rehling P

Thu Nov 11 00:00:00 EST 2021

N6-methyladenosine reader IMP2 stabilizes the ZFAS1/OLA1 axis and activates the Warburg effect: implication in colorectal cancer.

Journal of hematology & oncology

Lu S,Han L,Hu X,Sun T,Xu D,Li Y,Chen Q,Yao W,He M,Wang Z,Wu H,Wei M

Sun Nov 07 00:00:00 EDT 2021

N6-methyladenosine reader IMP2 stabilizes the ZFAS1/OLA1 axis and activates the Warburg effect: implication in colorectal cancer.

Journal of hematology & oncology

Lu S,Han L,Hu X,Sun T,Xu D,Li Y,Chen Q,Yao W,He M,Wang Z,Wu H,Wei M

Sun Nov 07 00:00:00 EDT 2021

Loss of Hilnc prevents diet-induced hepatic steatosis through binding of IGF2BP2.

Nature metabolism

Jiang Y,Peng J,Song J,He J,Jiang M,Wang J,Ma L,Wang Y,Lin M,Wu H,Zhang Z,Gao D,Zhao Y

Mon Nov 01 00:00:00 EDT 2021

Loss of Hilnc prevents diet-induced hepatic steatosis through binding of IGF2BP2.

Nature metabolism

Jiang Y,Peng J,Song J,He J,Jiang M,Wang J,Ma L,Wang Y,Lin M,Wu H,Zhang Z,Gao D,Zhao Y

Mon Nov 01 00:00:00 EDT 2021

Human m⁶A-mRNA and lncRNA epitranscriptomic microarray reveal function of RNA methylation in hemoglobin H-constant spring disease.

Scientific reports

Ruan H,Yang F,Deng L,Yang D,Zhang X,Li X,Pang L

Thu Oct 14 00:00:00 EDT 2021

lncRNA HCG11 suppresses human osteosarcoma growth through upregulating p27 Kip1.

Aging

Gu J,Dai B,Shi X,He Z,Xu Y,Meng X,Zhu J

Mon Sep 13 00:00:00 EDT 2021

lncRNA HCG11 suppresses human osteosarcoma growth through upregulating p27 Kip1.

Aging

Gu J,Dai B,Shi X,He Z,Xu Y,Meng X,Zhu J

Mon Sep 13 00:00:00 EDT 2021

EGR2-mediated regulation of m⁶A reader IGF2BP proteins drive RCC tumorigenesis and metastasis via enhancing S1PR3 mRNA stabilization.

Cell death & disease

Ying Y,Ma X,Fang J,Chen S,Wang W,Li J,Xie H,Wu J,Xie B,Liu B,Wang X,Zheng X,Xie L

Thu Jul 29 00:00:00 EDT 2021

EGR2-mediated regulation of m⁶A reader IGF2BP proteins drive RCC tumorigenesis and metastasis via enhancing S1PR3 mRNA stabilization.

Cell death & disease

Ying Y,Ma X,Fang J,Chen S,Wang W,Li J,Xie H,Wu J,Xie B,Liu B,Wang X,Zheng X,Xie L

Thu Jul 29 00:00:00 EDT 2021

linc01305 promotes metastasis and proliferation of esophageal squamous cell carcinoma through interacting with IGF2BP2 and IGF2BP3 to stabilize HTR3A mRNA.

The international journal of biochemistry & cell biology

Huang GW,Chen QQ,Ma CC,Xie LH,Gu J

Thu Jul 01 00:00:00 EDT 2021

The m6A Reader IGF2BP2 Regulates Macrophage Phenotypic Activation and Inflammatory Diseases by Stabilizing TSC1 and PPARγ.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Wang X,Ji Y,Feng P,Liu R,Li G,Zheng J,Xue Y,Wei Y,Ji C,Chen D,Li J

Thu Jul 01 00:00:00 EDT 2021

N6-Methyladenosine Modification of LncRNA DUXAP9 Promotes Renal Cancer Cells Proliferation and Motility by Activating the PI3K/AKT Signaling Pathway.

Frontiers in oncology

Tan L,Tang Y,Li H,Li P,Ye Y,Cen J,Gui C,Luo J,Cao J,Wei J

Sat Jun 26 00:00:00 EDT 2021

m6A modification promotes miR-133a repression during cardiac development and hypertrophy via IGF2BP2.

Cell death discovery

Qian B,Wang P,Zhang D,Wu L

Sat Jun 26 00:00:00 EDT 2021

m6A modification promotes miR-133a repression during cardiac development and hypertrophy via IGF2BP2.

Cell death discovery

Qian B,Wang P,Zhang D,Wu L

Sat Jun 26 00:00:00 EDT 2021

m⁶ A modification of lncRNA PCAT6 promotes bone metastasis in prostate cancer through IGF2BP2-mediated IGF1R mRNA stabilization.

Clinical and translational medicine

Lang C,Yin C,Lin K,Li Y,Yang Q,Wu Z,Du H,Ren D,Dai Y,Peng X

Tue Jun 01 00:00:00 EDT 2021

IGF2BP2 knockdown inhibits LPS-induced pyroptosis in BEAS-2B cells by targeting caspase 4, a crucial molecule of the non-canonical pyroptosis pathway.

Experimental and therapeutic medicine

Wang J,Yuan X,Ding N

Tue Jun 01 00:00:00 EDT 2021

Corrigendum to article "Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation''.

Nucleic acids research

Chen R,Liu Y,Zhuang H,Yang B,Hei K,Xiao M,Hou C,Gao H,Zhang X,Jia C,Li L,Li Y,Zhang N

Mon Apr 19 00:00:00 EDT 2021

Oncogenic AURKA-enhanced N⁶-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.

Cell research

Peng F,Xu J,Cui B,Liang Q,Zeng S,He B,Zou H,Li M,Zhao H,Meng Y,Chen J,Liu B,Lv S,Chu P,An F,Wang Z,Huang J,Zhan Y,Liao Y,Lu J,Xu L,Zhang J,Sun Z,Li Z,Wang F,Lam EW,Liu Q

Mon Mar 01 00:00:00 EST 2021

Oncogenic AURKA-enhanced N⁶-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.

Cell research

Peng F,Xu J,Cui B,Liang Q,Zeng S,He B,Zou H,Li M,Zhao H,Meng Y,Chen J,Liu B,Lv S,Chu P,An F,Wang Z,Huang J,Zhan Y,Liao Y,Lu J,Xu L,Zhang J,Sun Z,Li Z,Wang F,Lam EW,Liu Q

Mon Mar 01 00:00:00 EST 2021

Oncogenic AURKA-enhanced N⁶-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.

Cell research

Peng F,Xu J,Cui B,Liang Q,Zeng S,He B,Zou H,Li M,Zhao H,Meng Y,Chen J,Liu B,Lv S,Chu P,An F,Wang Z,Huang J,Zhan Y,Liao Y,Lu J,Xu L,Zhang J,Sun Z,Li Z,Wang F,Lam EW,Liu Q

Mon Mar 01 00:00:00 EST 2021

LINC00460/DHX9/IGF2BP2 complex promotes colorectal cancer proliferation and metastasis by mediating HMGA1 mRNA stability depending on m6A modification.

Journal of experimental & clinical cancer research : CR

Hou P,Meng S,Li M,Lin T,Chu S,Li Z,Zheng J,Gu Y,Bai J

Mon Feb 01 00:00:00 EST 2021

LINC00460/DHX9/IGF2BP2 complex promotes colorectal cancer proliferation and metastasis by mediating HMGA1 mRNA stability depending on m6A modification.

Journal of experimental & clinical cancer research : CR

Hou P,Meng S,Li M,Lin T,Chu S,Li Z,Zheng J,Gu Y,Bai J

Mon Feb 01 00:00:00 EST 2021

Multiomics integrative analysis for gene signatures and prognostic values of m⁶A regulators in pancreatic adenocarcinoma: a retrospective study in The Cancer Genome Atlas project.

Aging

Gao W,Cheng L,He S,Li W,Zhou C,Zhou B,Liu J,Xu J,Yu X,Zhu H

Tue Oct 20 00:00:00 EDT 2020

Multiomics integrative analysis for gene signatures and prognostic values of m⁶A regulators in pancreatic adenocarcinoma: a retrospective study in The Cancer Genome Atlas project.

Aging

Gao W,Cheng L,He S,Li W,Zhou C,Zhou B,Liu J,Xu J,Yu X,Zhu H

Tue Oct 20 00:00:00 EDT 2020

Clinical Significance of an m6A Reader Gene, IGF2BP2, in Head and Neck Squamous Cell Carcinoma.

Frontiers in molecular biosciences

Deng X,Jiang Q,Liu Z,Chen W

Mon Sep 28 00:00:00 EDT 2020

Functional characterization of the long noncoding RNA MIR22HG as a tumour suppressor in cervical cancer by targeting IGF2BP2.

European review for medical and pharmacological sciences

Shu J,Wang D

Sat Aug 01 00:00:00 EDT 2020

IGF2BP2 regulates DANCR by serving as an N6-methyladenosine reader.

Cell death and differentiation

Hu X,Peng WX,Zhou H,Jiang J,Zhou X,Huang D,Mo YY,Yang L

Mon Jun 01 00:00:00 EDT 2020

Wilms' tumour 1-associating protein inhibits endothelial cell angiogenesis by m6A-dependent epigenetic silencing of desmoplakin in brain arteriovenous malformation.

Journal of cellular and molecular medicine

Wang LJ,Xue Y,Li H,Huo R,Yan Z,Wang J,Xu H,Wang J,Cao Y,Zhao JZ

Fri May 01 00:00:00 EDT 2020

Up-regulation of IGF2BP2 by multiple mechanisms in pancreatic cancer promotes cancer proliferation by activating the PI3K/Akt signaling pathway.

Journal of experimental & clinical cancer research : CR

Xu X,Yu Y,Zong K,Lv P,Gu Y

Wed Dec 18 00:00:00 EST 2019

N⁶-methyladenosine modification of circNSUN2 facilitates cytoplasmic export and stabilizes HMGA2 to promote colorectal liver metastasis.

Nature communications

Chen RX,Chen X,Xia LP,Zhang JX,Pan ZZ,Ma XD,Han K,Chen JW,Judde JG,Deas O,Wang F,Ma NF,Guan X,Yun JP,Wang FW,Xu RH

Wed Oct 16 00:00:00 EDT 2019

N⁶-methyladenosine modification of circNSUN2 facilitates cytoplasmic export and stabilizes HMGA2 to promote colorectal liver metastasis.

Nature communications

Chen RX,Chen X,Xia LP,Zhang JX,Pan ZZ,Ma XD,Han K,Chen JW,Judde JG,Deas O,Wang F,Ma NF,Guan X,Yun JP,Wang FW,Xu RH

Wed Oct 16 00:00:00 EDT 2019

N⁶-methyladenosine modification of circNSUN2 facilitates cytoplasmic export and stabilizes HMGA2 to promote colorectal liver metastasis.

Nature communications

Chen RX,Chen X,Xia LP,Zhang JX,Pan ZZ,Ma XD,Han K,Chen JW,Judde JG,Deas O,Wang F,Ma NF,Guan X,Yun JP,Wang FW,Xu RH

Wed Oct 16 00:00:00 EDT 2019

Insulin-like growth factor 2 mRNA binding protein 2 promotes aerobic glycolysis and cell proliferation in pancreatic ductal adenocarcinoma via stabilizing GLUT1 mRNA.

Acta biochimica et biophysica Sinica

Huang S,Wu Z,Cheng Y,Wei W,Hao L

Wed Jul 10 00:00:00 EDT 2019

The HMGA2-IMP2 Pathway Promotes Granulosa Cell Proliferation in Polycystic Ovary Syndrome.

The Journal of clinical endocrinology and metabolism

Li M,Zhao H,Zhao SG,Wei DM,Zhao YR,Huang T,Muhammad T,Yan L,Gao F,Li L,Lu G,Chan WY,Leung PCK,Dunaif A,Liu HB,Chen ZJ

Mon Apr 01 00:00:00 EDT 2019

Airn Regulates Igf2bp2 Translation in Cardiomyocytes.

Circulation research

Hosen MR,Militello G,Weirick T,Ponomareva Y,Dassanayaka S,Moore JB,Döring C,Wysoczynski M,Jones SP,Dimmeler S,Uchida S

Fri May 11 00:00:00 EDT 2018

Airn Regulates Igf2bp2 Translation in Cardiomyocytes.

Circulation research

Hosen MR,Militello G,Weirick T,Ponomareva Y,Dassanayaka S,Moore JB,Döring C,Wysoczynski M,Jones SP,Dimmeler S,Uchida S

Fri May 11 00:00:00 EDT 2018

Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation.

Nucleic acids research

Chen R,Liu Y,Zhuang H,Yang B,Hei K,Xiao M,Hou C,Gao H,Zhang X,Jia C,Li L,Li Y,Zhang N

Fri Sep 29 00:00:00 EDT 2017

Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation.

Nucleic acids research

Chen R,Liu Y,Zhuang H,Yang B,Hei K,Xiao M,Hou C,Gao H,Zhang X,Jia C,Li L,Li Y,Zhang N

Fri Sep 29 00:00:00 EDT 2017

IMP2 axonal localization, RNA interactome, and function in the development of axon trajectories.

Development (Cambridge, England)

Preitner N,Quan J,Li X,Nielsen FC,Flanagan JG

Mon Aug 01 00:00:00 EDT 2016

IMP2 axonal localization, RNA interactome, and function in the development of axon trajectories.

Development (Cambridge, England)

Preitner N,Quan J,Li X,Nielsen FC,Flanagan JG

Mon Aug 01 00:00:00 EDT 2016

Mesenchymal Tumorigenesis Driven by TSC2 Haploinsufficiency Requires HMGA2 and Is Independent of mTOR Pathway Activation.

Cancer research

D'Armiento J,Shiomi T,Marks S,Geraghty P,Sankarasharma D,Chada K

Mon Feb 15 00:00:00 EST 2016

Mesenchymal Tumorigenesis Driven by TSC2 Haploinsufficiency Requires HMGA2 and Is Independent of mTOR Pathway Activation.

Cancer research

D'Armiento J,Shiomi T,Marks S,Geraghty P,Sankarasharma D,Chada K

Mon Feb 15 00:00:00 EST 2016

Role of IGF2BP3 in trophoblast cell invasion and migration.

Cell death & disease

Li W,Liu D,Chang W,Lu X,Wang YL,Wang H,Zhu C,Lin HY,Zhang Y,Zhou J,Wang H

Thu Jan 23 00:00:00 EST 2014

Role of IGF2BP3 in trophoblast cell invasion and migration.

Cell death & disease

Li W,Liu D,Chang W,Lu X,Wang YL,Wang H,Zhu C,Lin HY,Zhang Y,Zhou J,Wang H

Thu Jan 23 00:00:00 EST 2014

Bioinformatics

Protein Aliases: Hepatocellular carcinoma autoantigen p62; IGF-II mRNA-binding protein 2; IGF2 mRNA-binding protein 2; insulin-like growth factor 2 mRNA binding protein 2; Insulin-like growth factor 2 mRNA-binding protein 2; insulin-like growth factor 2, binding protein 2; VICKZ family member 2

Gene Aliases: C330012H03Rik; IGF2BP2; IMP-2; IMP2; Neilsen; RGD1305614; VICKZ2

UniProt ID: (Human) Q9Y6M1, (Mouse) Q5SF07

Entrez Gene ID: (Human) 10644, (Mouse) 319765, (Rat) 303824

Molecular Function: RNA metabolism protein