Immunofluorescent analysis of IGF2BP2 showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using an IGF2BP2 polyclonal antibody (Product # PA5-29869) diluted at 1:500. Blue: Hoechst 33343 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 260 of Human IGF2BP2|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-29869 targets IGF2BP2 in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-29869 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 260 of Human IGF2BP2.
This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains several four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
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