Proteintech

IGF2BP3 Polyclonal Antibody

IGF2BP3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

IGF2BP3 Antibody (14642-1-AP) in ICC/IF

Immunofluorescent analysis of MCF-7 cells, using IGF2BP3 antibody 14642-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

IGF2BP3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

IGF2BP3 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14642-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 54 publications 54 publications

Immunohistochemistry (IHC)

View 15 publications 15 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 7 publications 7 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

RNA Immunoprecipitation (RIP)

View 14 publications 14 publications

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

IGF2BP3 Fusion Protein Ag6226 (240-579 aa encoded by BC065269)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.15 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Both isoforms (64 kDa and 22 kDa) of IGFBP3 can be recognized by this antibody. IGFBP3 is nuclear and cytoplasmic in staining.

Immunogen sequence: AEKSITILS TPEGTSAACK SILEIMHKEA QDIKFTEEIP LKILAHNNFV GRLIGKEGRN LKKIEQDTDT KITISPLQEL TLYNPERTIT VKGNVETCAK AEEEIMKKIR ESYENDIASM NLQAHLIPGL NLNALGLFPP TSGMPPPTSG PPSAMTPPYP QFEQSETETV HLFIPALSVG AIIGKQGQHI KQLSRFAGAS IKIAPAEAPD AKVRMVIITG PPEAQFKAQG RIYGKIKEEN FVSPKEEVKL EAHIRVPSFA AGRVIGKGGK TVNELQNLSS AEVVVPRDQT PDENDQVVVK ITGHFYACQV AQRKIQEILT QVKQHQQQKA LQSGPPQSRR K (240-579 aa encoded by BC065269)

Target Information

Insulin-like growth factor-II messenger RNA (mRNA)-binding protein-3 (IMP-3), also known as K homology domain-containing protein overexpressed in cancer (KOC) and L523S, is a member of the insulin-like growth factor-II mRNA-binding protein family and is expressed during embryogenesis and in some malignancies. IMP-3 is expressed in malignant melanoma but not in benign nevi, even when dysplastic features are present; IMP-3 is expressed in a significantly higher proportion of melanomas than Spitz nevi; and IMP-3 is expressed in metastatic melanomas significantly more than in thin melanomas. IMP-3 appears to be involved in the progression of malignant melanoma and may play an important role in the regulation of the biologic behavior of this tumor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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RNA N6-methyladenosine reader IGF2BP3 interacts with MYCN and facilitates neuroblastoma cell proliferation.

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