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Invitrogen
IGF2R Polyclonal Antibody
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FIGURE: 1 / 8
IGF2R Antibody (PA3-850) in ICC/IF
Immunofluorescent analysis of CI-MPR (green) showing staining in the cytoplasm and nucleus of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CI-MPR polyclonal antibody (Product # PA3-850) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a Dy... View More
Product Details
PA3-850
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Non-human primate
Published species
Human,
Mouse,
Non-human primate
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
300 kDa cation-independent receptor purified from adult bovine liver tissue.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA3-850 detects cation-independent mannose-6-receptor(CI-MPR) in human, bovine, mouse and non-human primate cells.
PA3-850 has been successfully used in ICC/IF, Dot blot and Western blotting procedures. By Western blot, it detects a 300 kDa protein representing CI-MPR under non-reducing conditions.
The PA3-850 immunogen is a 300 kDa cation-independent receptor purified from adult Bovine liver tissue.
Target Information
Lysosomal enzymes containing one or two mannose 6-phosphate (man6P) moieties are moved about in the cell by two distinct but interconnected cycles by means of approximately 300 kDa cation independent mannose 6-phospate receptors (CI-MPR). The biosynthetic cycle refers to the MPR transport of newly synthesized lysosomal enzymes from the trans Golgi network to late endosomes or early lysosomes. The endocytic cycle transports extracellular lysosomal enzymes from the plasma membrane via clathrin-coated vesicles to early endosomes. The entire pool of MPRs cycles between these cellular compartments every 3 hours. The steady state distribution of MPR's is predominantly within late endosomes, fewer in the trans Golgi network and approximately 10 % at cell surface. In addition to its man6P binding activity, the MPR contains a separate binding site for the type II insulin-like growth factor and is capable of binding both man6P and IGF-II simultaneously. An approximately 240 kDa soluble, truncated form, representing the extracellular domain of the protein, has also been found circulating in serum and is capable of binding both ligands.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 300 kDa mannose 6-phosphate receptor; cation-independent mannose 6-phosphate receptor; cation-independent mannose 6-phosphate receptor protein; Cation-independent mannose-6-phosphate receptor; cation-independent MPR; CD222; CI Man-6-P receptor; IGF-II receptor; IGF-II/CI-MPR; Insulin-like growth factor 2 receptor; Insulin-like growth factor II receptor; insulin-like growth factor receptor type 2; M6P/IGF2 receptor; M6P/IGF2R; M6PR; mannose-6-phosphate receptor, cation independent; MPR 300
Gene Aliases: AI661837; CD222; CI-M6PR; CI-MPR; CIMPR; IGF2R; M6P; M6P-R; M6P/IGF2R; MPR 300; MPR1; MPR300; MPRI
UniProt ID: (Human) P11717, (Bovine) P08169, (Mouse) Q07113
Entrez Gene ID: (Human) 3482, (Bovine) 281849, (Mouse) 16004
glycoprotein binding
G-protein alpha-subunit binding
retinoic acid binding
receptor activity
G-protein coupled receptor activity
insulin-like growth factor-activated receptor activity
transporter activity
protein binding
mannose binding
enzyme binding
insulin-like growth factor II binding
identical protein binding
phosphoprotein binding
insulin-like growth factor binding
kringle domain binding
signaling receptor activity
membrane traffic protein
liver development
receptor-mediated endocytosis
signal transduction
G-protein coupled receptor signaling pathway
spermatogenesis
post-embryonic development
organ regeneration
response to retinoic acid
positive regulation of apoptotic process
insulin-like growth factor receptor signaling pathway
lysosomal transport
transport
regulation of apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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