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IGHMBP2 Antibody (PA5-116653) in WB
Western blot analysis of IGHMBP2 in extracts of various cell lines. Samples were incubated with IGHMBP2 Polyclonal antibody (Product # PA5-116653) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-116653
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 540-720 of human IGHMBP2
Conjugate
Unconjugated
Form
Liquid
Concentration
2.72 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
RRID
AB_2901284
Product Specific Information
Positive Samples: HepG2, A-549, Mouse brain, Mouse testis, Rat brain
Target Information
5' to 3' helicase that unwinds RNA and DNA duplices in an ATP-dependent reaction. Acts as a transcription regulator. Required for the transcriptional activation of the flounder liver-type antifreeze protein gene. Exhibits strong binding specificity to the enhancer element B of the flounder antifreeze protein gene intron. Binds to the insulin II gene RIPE3B enhancer region. May be involved in translation. DNA-binding protein specific to 5'-phosphorylated single-stranded guanine-rich sequence related to the immunoglobulin mu chain switch region. Preferentially binds to the 5'-GGGCT-3' motif. Interacts with tRNA-Tyr. Stimulates the transcription of the human neurotropic virus JCV.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AEP; antifreeze enhancer-binding protein; Antifreeze enhancer-binding protein ortholog; ATP-dependent helicase IGHMBP2; cardiac transcription factor 1; DNA-binding protein SMUBP-2; GF-1; Glial factor 1; Immunoglobulin mu-binding protein 2; immunoglobulin S mu binding protein 2; neuromuscular degeneration; p110 subunit; zinc finger, AN1-type domain 7
Gene Aliases: AEP; CATF1; CMT2S; HCSA; HMN6; IGHMBP2; nmd; RIPE3b1; sma; SMARD1; Smbp-2; SMBP2; SMUBP2; ZFAND7
UniProt ID: (Human) P38935, (Rat) Q9EQN5
Entrez Gene ID: (Human) 3508, (Mouse) 20589, (Rat) 29532
Molecular Function:
DNA metabolism protein
DNA replication
DNA repair
DNA recombination
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
translation
DNA duplex unwinding
protein homooligomerization
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
spinal cord motor neuron differentiation
metabolic process
tRNA binding
DNA binding
DNA helicase activity
single-stranded DNA binding
RNA binding
protein binding
ATP binding
DNA-dependent ATPase activity
transcription factor binding
RNA-dependent ATPase activity
zinc ion binding
ATP-dependent 5'-3' RNA helicase activity
ribosome binding
ATP-dependent 5'-3' DNA helicase activity
nucleotide binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
nucleic acid binding
helicase activity
hydrolase activity
metal ion binding
protein self-association
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