Proteintech

IHH Polyclonal Antibody

View all (9) IHH antibodies

Questions & Answers

Tech Support

Questions & Answers

Tech Support

Product Image Gallery

IHH Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human stomach cancer tissue slide using 13388-1-AP (IHH Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

IHH Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human stomach cancer tissue slide using 13388-1-AP (IHH Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

IHH Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney tissue slide using 13388-1-AP ( IHH Antibody) at dilution of 1:200 (under 40x lens).

IHH Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney tissue slide using 13388-1-AP ( IHH Antibody) at dilution of 1:200 (under 10x lens).

IHH Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human stomach cancer using 13388-1-AP (IHH antibody) at dilution of 1:50 (under 40x lens).

IHH Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human stomach cancer using 13388-1-AP (IHH antibody) at dilution of 1:50 (under 10x lens).

IHH Antibody in Western Blot (WB) — Human kidney tissue were subjected to SDS PAGE followed by western blot with 13388-1-AP (IHH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

IHH Antibody in Western Blot (WB) — Human heart tissue were subjected to SDS PAGE followed by western blot with 13388-1-AP (IHH antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Product Details

13388-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

-

Immunocytochemistry (ICC/IF)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

IHH Fusion Protein Ag4046 (103-411 aa encoded by BC034757)

Conjugate

Unconjugated

Form

Liquid

Concentration

0.24 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: TQRCKDRLN SLAISVMNQW PGVKLRVTEG WDEDGHHSEE SLHYEGRAVD ITTSDRDRNK YGLLARLAVE AGFDWVYYES KAHVHCSVKS EHSAAAKTGG CFPAGAQVRL ESGARVALSA VRPGDRVLAM GEDGSPTFSD VLIFLDREPH RLRAFQVIET QDPPRRLALT PAHLLFTADN HTEPAARFRA TFASHVQPGQ YVLVAGVPGL QPARVAAVST HVALGAYAPL TKHGTLVVED VVASCFAAVA DHHLAQLAFW PLRLFHSLAW GSWTPGEGVH WYPQLLYRLG RLLLEEGSFH PLGMSGAGS (103-411 aa encoded by BC034757)

Target Information

Ihh encodes a member of the hedgehog family of secreted signaling molecules. Hedgehog proteins are essential regulators of a variety of developmental processes including growth, patterning and morphogenesis. The encoded protein specifically plays a role in bone growth an differentiation. Mutations in this gene are the cause of brachydactyly type A1 which is characterized by shortening or malformation of the phalanges. Mutations in this gene are also the cause of acrocapitofemoral dysplasia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Primary cilia support cartilage regeneration after injury.

International journal of oral science

Tao D,Zhang L,Ding Y,Tang N,Xu X,Li G,Niu P,Yue R,Wang X,Shen Y,Sun Y

Fri Jun 02 00:00:00 EDT 2023

Hedgehog Signalling Contributes to Trauma-Induced Tendon Heterotopic Ossification and Regulates Osteogenesis through Antioxidant Pathway in Tendon-Derived Stem Cells.

Antioxidants (Basel, Switzerland)

Li G,Deng Y,Li K,Liu Y,Wang L,Wu Z,Chen C,Zhang K,Yu B

Wed Nov 16 00:00:00 EST 2022

DDB1 promotes the proliferation and hypertrophy of chondrocytes during mouse skeleton development.

Developmental biology

Zhao L,Wang X,Pomlok K,Liao H,Yang G,Yang X,Chen YG

Tue Sep 15 00:00:00 EDT 2020

Adamts17 is involved in skeletogenesis through modulation of BMP-Smad1/5/8 pathway.

Cellular and molecular life sciences : CMLS

Oichi T,Taniguchi Y,Soma K,Oshima Y,Yano F,Mori Y,Chijimatsu R,Kim-Kaneyama JR,Tanaka S,Saito T

Sun Dec 01 00:00:00 EST 2019

Inhibition of Ihh Reverses Temporomandibular Joint Osteoarthritis via a PTH1R Signaling Dependent Mechanism.

International journal of molecular sciences

Yang H,Zhang M,Liu Q,Zhang H,Zhang J,Lu L,Xie M,Chen D,Wang M

Sat Aug 03 00:00:00 EDT 2019

Inhibition of Ihh Reverses Temporomandibular Joint Osteoarthritis via a PTH1R Signaling Dependent Mechanism.

International journal of molecular sciences

Yang H,Zhang M,Liu Q,Zhang H,Zhang J,Lu L,Xie M,Chen D,Wang M

Sat Aug 03 00:00:00 EDT 2019

TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.

Nature communications

Zhu R,Gires O,Zhu L,Liu J,Li J,Yang H,Ju G,Huang J,Ge W,Chen Y,Lu Z,Wang H

Fri Jun 28 00:00:00 EDT 2019

mTORC1 activation downregulates FGFR3 and PTH/PTHrP receptor in articular chondrocytes to initiate osteoarthritis.

Osteoarthritis and cartilage

Zhang H,Wang H,Zeng C,Yan B,Ouyang J,Liu X,Sun Q,Zhao C,Fang H,Pan J,Xie D,Yang J,Zhang T,Bai X,Cai D

Thu Jun 01 00:00:00 EDT 2017

mTORC1 activation downregulates FGFR3 and PTH/PTHrP receptor in articular chondrocytes to initiate osteoarthritis.

Osteoarthritis and cartilage

Zhang H,Wang H,Zeng C,Yan B,Ouyang J,Liu X,Sun Q,Zhao C,Fang H,Pan J,Xie D,Yang J,Zhang T,Bai X,Cai D

Thu Jun 01 00:00:00 EDT 2017

Intra-articular Delivery of Antago-miR-483-5p Inhibits Osteoarthritis by Modulating Matrilin 3 and Tissue Inhibitor of Metalloproteinase 2.

Molecular therapy : the journal of the American Society of Gene Therapy

Wang H,Zhang H,Sun Q,Wang Y,Yang J,Yang J,Zhang T,Luo S,Wang L,Jiang Y,Zeng C,Cai D,Bai X

Wed Mar 01 00:00:00 EST 2017

Intra-articular Delivery of Antago-miR-483-5p Inhibits Osteoarthritis by Modulating Matrilin 3 and Tissue Inhibitor of Metalloproteinase 2.

Molecular therapy : the journal of the American Society of Gene Therapy

Wang H,Zhang H,Sun Q,Wang Y,Yang J,Yang J,Zhang T,Luo S,Wang L,Jiang Y,Zeng C,Cai D,Bai X

Wed Mar 01 00:00:00 EST 2017

Bioinformatics

Protein Aliases: HHG-2; IHH; Indian hedgehog homolog; Indian hedgehog protein; Indian hedgehog protein N-product

Gene Aliases: BDA1; HHG2; IHH

UniProt ID: (Human) Q14623, (Mouse) Q61724

Entrez Gene ID: (Human) 3549, (Mouse) 16147, (Rat) 84399

skeletal system development ossification patterning of blood vessels osteoblast differentiation in utero embryonic development cell fate specification morphogenesis of a branching structure vasculature development heart looping positive regulation of mesenchymal cell proliferation epithelial cell morphogenesis retinal pigment epithelium development proteoglycan metabolic process proteolysis cell communication smoothened signaling pathway cell-cell signaling multicellular organism development pattern specification process positive regulation of cell proliferation embryo development embryonic pattern specification negative regulation of signal transduction intein-mediated protein splicing cell differentiation vitelline membrane formation pancreas development positive regulation of chondrocyte differentiation positive regulation of collagen biosynthetic process negative regulation of T cell differentiation in thymus negative regulation of immature T cell proliferation in thymus positive regulation of T cell differentiation in thymus multicellular organism growth chondrocyte proliferation regulation of growth embryonic digit morphogenesis camera-type eye development negative regulation of apoptotic process bone resorption regulation of cell differentiation negative regulation of cell differentiation positive regulation of smoothened signaling pathway positive regulation of transcription from RNA polymerase II promoter positive regulation of alpha-beta T cell differentiation negative regulation of alpha-beta T cell differentiation negative regulation of eye pigmentation cell maturation embryonic digestive tract morphogenesis embryonic camera-type eye morphogenesis neuron development smooth muscle tissue development positive regulation of epithelial cell proliferation cartilage development camera-type eye photoreceptor cell fate commitment head morphogenesis somite development embryonic skeletal joint development epithelial cell-cell adhesion response to estradiol maternal process involved in female pregnancy

Process(es)

patched binding calcium ion binding peptidase activity protein binding hydrolase activity metal ion binding molecular_function